Difference between revisions of "Team:ETH Zurich/Parts"

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<h2>Most important parts from the Registry </h2>
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<h2>Newly characterized parts the Registry </h2>
 
<p>Here we present the parts from the Registry that were essential for our system.</p>
 
<p>Here we present the parts from the Registry that were essential for our system.</p>
 
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<td>pLldR</td>
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<td>lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. <b>Check out our <a href="http://parts.igem.org/Part:BBa_K288000">characterization for pLldR</a>!</b></td>
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<td><a href="http://parts.igem.org/Part:BBa_K822000">BBa_K822000</a></td>
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</table>
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<h2>Used parts from the Registry</h2>
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<p>In this section we present other parts from the Registry that we used during our project.</p>
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<th><h4>Part Name</h4></th>
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<th><h4>Description</h4></th>
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<th><h4>Registry Number</h4></th>
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</tr>
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<td>INP-EYFP</td>
 
<td>INP-EYFP</td>
 
<td>Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP). <b><a href="http://parts.igem.org/Part:BBa_K523013">Check out our use of INP</b>!</b></td>
 
<td>Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP). <b><a href="http://parts.igem.org/Part:BBa_K523013">Check out our use of INP</b>!</b></td>
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<td>pLldR operon</td>
 
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<td>lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression</td>
 
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<td><a href="http://parts.igem.org/Part:BBa_K822000">BBa_K822000</a></td>
 
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</table>
 
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<h2>Other parts from the Registry parts </h2>
 
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<p>In this section we present other parts from the Registry that we used during our project.</p>
 
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<table>
 
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<tr>
 
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<th><h4>Part Name</h4></th>
 
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<th><h4>Description</h4></th>
 
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<th><h4>Registry Number</h4></th>
 
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Revision as of 11:05, 18 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Parts

For completing our project, we required of many parts. Some of these parts where used from the Registry, others were designed and cloned for this project. In the following section, you can see these parts.

Overview of our genetic design.

Newly characterized parts the Registry

Here we present the parts from the Registry that were essential for our system.

Part Name

Description

Registry Number
aiiA Autoinducer inactivation enzyme aiiA (enzyme that inactivates the acylhomoserine lactone (AHL) quorum-sensing signal). Check out our characterization for aiiA! BBa_C0160
pLldR lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. Check out our characterization for pLldR! BBa_K822000

Used parts from the Registry

In this section we present other parts from the Registry that we used during our project.

Part Name

Description

Registry Number
INP-EYFP Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP). Check out our use of INP! BBa_K523013
lacI + LVA lacI repressor from E. coli (+LVA) BBa_C0012
pLuxR Promoter activated by the LuxR protein complexed with the autoinducer homoserine lactone (HSL) BBa_R0062
cI cI repressor from E. coli phage lambda modified with an LVA tail for rapid degradation of the protein BBa_C0051
HylA HlyA-tag+Secretion system, allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli BBa_K1166002
InterLab 1 strong promoter Anderson promoter J23101 from the Anderson collection BBa_K823005 (BBa_J23101)
InterLab 2 medium-strong promoter Anderson promoter J23106 from the Anderson collection BBa_K823008 (BBa_J23106)
terminator Transcription terminator for the E.coli RNA polymerase BBa_B0012
double terminator Double terminator consisting of BBa_B0010 and BBa_B0012 BBa_B0015
very strong promoter Strong member of the family of promoters J23100 through J23119 BBa_J23100
gfp (InterLab) intermediate in screening plasmid construction containing GFP BBa_I13504
medium promoter Medium member of the family of promoters J23100 through J23119 BBa_J23118
LuxI autoinducer synthetase for acylhomoserine lactone (AHL), no LVA BBa_C0161
InterLab 3 weak promoter Anderson promoter J23117 from the Anderson collection BBa_K823013 (BBa_J23117)
promoter medium-weak Medium-weak member of the family of promoters J23100 through J23119 BBa_J23114
RBS RBS.3 (medium) derivative of BBa_0030 BBa_B0032
promoter plus GFP (InterLab control) J23151 inserted in the Promoter MeasKit BBa_I20270
terminator Artificial terminator, estimated %T~>90% BBa_B1006

We would like to thank our sponsors