Difference between revisions of "Team:Cork Ireland/Parts"
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Revision as of 11:10, 18 September 2015
55bp HPV Detector (K1698001)
Sequence: CTCTTCAcctgtgtaggtgttgaggtaggtcgtggGTACCAATAATAAAAGCTt cagccattaggtgtgggcattagtggCACTGC
Part may be activated by established protocol to become a detector plasmid for a 55bp Target sequence, found on the HPV L1 Capsid gene.
For protocol for construction of detector by digestion of plasmid see HERE. The Target is located in the HPV genome flanked by HaeIII restriction sites. Detector may work best if genomic sample first digested with this enzyme to create target fragment of the correct length. The part was cloned into a plasmid containing BBa_K584001 to allow examination of cells following successful transformation using a fluorescent microscope. Also, presence of GFP expressing part meant that detector colonies could be differentiated from control colonies. For more information see (STANDARDISATION OF RESULTS section)
The sequence of this part with K584001 biobrick part may be found on the Registry of Standsrd Parts.
For more information on how the detector plasmid worked see (RESULTS : DETECTORS)
Assessment of this detector parts selectivity may also be seen here (RESULTS : SELECTIVITY)
