Difference between revisions of "Team:FAU Erlangen/Tour34"
Adrian FAU (Talk | contribs) |
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<h1>Set backs </h1> | <h1>Set backs </h1> | ||
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| + | <h4>Sequences</h4> | ||
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| + | <ul> | ||
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| + | <li> We planned to work with three different loci, yet forgot to synthesize a selectionmarker <b> within</b> | ||
| + | the region for the recombination. | ||
| + | |||
| + | <li>Due to misunderstanding the only working homologous insert for the can1 gene was not used. | ||
| + | </ul> | ||
<h4>rpd3 </h4> | <h4>rpd3 </h4> | ||
Revision as of 11:52, 18 September 2015
Set backs
Sequences
- We planned to work with three different loci, yet forgot to synthesize a selectionmarker within the region for the recombination.
- Due to misunderstanding the only working homologous insert for the can1 gene was not used.
rpd3
- the rpd3 clones sent for sequencing always contained mutations
Golden Gate
- we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production
- accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity
