Difference between revisions of "Team:HokkaidoU Japan/Composite Part"
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<br><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1714000">BBa_K1714000</a></br> | <br><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1714000">BBa_K1714000</a></br> | ||
| − | <p>Our favorite composite part is BBa_K1714000. This part has pBad/araC as a promoter, BBa_B0034 as an RBS, Ag43 thanatin as α-domain as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production, we thought 1mer version | + | <p>Our favorite composite part is BBa_K1714000. This part has pBad/araC as a promoter, BBa_B0034 as an RBS, Ag43 thanatin as α-domain as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production, we thought 1mer version is the our best composite part. |
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Revision as of 15:07, 18 September 2015
Composite Part
BBa_K1714000
Our favorite composite part is BBa_K1714000. This part has pBad/araC as a promoter, BBa_B0034 as an RBS, Ag43 thanatin as α-domain as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production, we thought 1mer version is the our best composite part.
