Difference between revisions of "Team:Peking/Attributions"

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<h2> Attributions</h2>
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            <div class="navbar navbar-default navbar-top">
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                            <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
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                        </button>
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                        <!-- End Toggle Nav Link For Mobiles -->
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                        <a class="navbar-brand" href="https://2015.igem.org/Team:Peking">
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                            <img alt="Peking iGEM 2015" src="https://static.igem.org/mediawiki/2015/8/8b/Peking-header-logo-1.png" style="height:55px;margin-top:7px">
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                    </div>
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                    <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
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                        <!-- Start Navigation List -->
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Achievements</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Design">Project</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
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                                    </li>
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<li><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired<span style="text-transform:lowercase"> d</span>Cas9 Reporter</a>                                   
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</li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Isothermal">Iso-<span style="text-transform:lowercase">t</span>hermal Amplification</a></li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Device">Hardware</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Speculation">Speculation</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Modeling">Modeling</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling">Array Design</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling/Analysis">Analysis algorithm</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Practices">Practices</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/Practices">Overview</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Background">TB Facts</a>
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                                    </li>
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                                    <li><a  href="https://2015.igem.org/Team:Peking/Practices/Consultation">Consultation and Interview</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Engagement">Public Engagement</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Outreach">Ethics and Economics Issues</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a  class="active" href="https://2015.igem.org/Team:Peking/Team">Lab</a>
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                                <ul>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Team">Team</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Notebook">Notebook</a>
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                                    </li>
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                                    <li><a class="active" href="https://2015.igem.org/Team:Peking/Attributions">Attributions</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Safety">Safety</a>
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                                    </li>
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                                </ul>
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                            </li>
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                        </ul>
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            <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0">Safety</h2>
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            <p style="margin-top:0px;font-size:14px">Better to be safe than sorry.</p>
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            <ul class="breadcrumbs">
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              <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
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              <li>Lab</li>
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            </ul>
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          </div>
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        </div>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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              <h4 style="font-size:18px">Safety<span class="head-line"></span></h4>
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                <ul>
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                <li>
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                  <a href="#Overview">Overview</a>
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                </li>
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                <li>
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                  <a href="#Lab">Safe Lab Work</a>
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                </li>
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                  <li>
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                  <a href="#Shipment">Safe Shipment</a>
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                </li>
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              </ul>
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            </div>
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              <h4 style="font-size:18px">Literature Investigation and Economical Assessment <span class="head-line"></span></h4>
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                <ul>
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                <li>
 +
                  <a href="#">Overview</a>
 +
                </li>
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                <li>
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                  <a href="#xxxx">Safe Lab Work</a>
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                </li>
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                  <li>
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                  <a href="#xxxx">Safe Shipment</a>
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                </li>
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              </ul>
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                <h3 class="classic-title" id="Overview" style="margin-top:50px;"><span>Member’s Attributions</span></h3>
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<div>               
 +
<p><b>ZHANG Yihao</b> is the team leader of 2015 Peking iGEM team. He participated in designing of the project, took charge of troubleshooting, and arranged the experimental schedule, making sure that the whole lab function smoothly and head in the right direction.<br/>
 +
<b>WEI Weijia</b> committed to ameliorate the detection system, that's to say, to enhance the sensitivity as well as to reduce the difficulty of detection. He was also in responsible for gathering plasmid information and seeking appropriate substrates.<br/>
 +
<b>LI Hang</b> was responsible for designing and manufacturing our devices, which are for detection of analogue electrical signal and optical signal.<br/>
 +
<b>CHENG Sida</b> was in charge of Human Practice and also works in the wet lab to construct and prepare plasmids for variable gRNA in vitro transcription and fusion protein construction.<br/>
 +
<b>LI Jiaofeng</b> worked as the financial manager of the team. She was also responsible for parts construction and submission.<br/>
 +
<b>LI Xiang</b> was in charge of constructing another kind of M.tuberculosis sensing system using Molecule Beacon. He was also engaged in the protein purification of dCas9 fusion protein.<br/>
 +
<b>LING Pingping</b> was in charge of testing the luminescence intensity of split firefly luciferase fused with CRISPR system. She also processed the whole experimental data.<br/>
 +
<b>LIU Dongming</b> helped to accomplish the plasmid construction of the detecting target and was responsible for human practice.<br/>
 +
<b>LIU Wenchao</b> was in charge of construction of CRISPR gRNA as well as RNA scaffold at the beginning. Lately she was working on protein purification of dCas9 fusion protein.<br/>
 +
<b>WANG Beining</b> undertook the task of cloning for the dCas9-luciferase part. She was also responsible for the testing of CRISPR-Luciferase system.<br/>
 +
<b>WANG Yu</b> participated in plasmid construction initially, protein purification, and wiki designing and building. She was also the designer of our PowerPoint.<br/>
 +
<b>XU Luze</b> was in charge of Modelling. His main work was programmed to search for the suitable guide sequences. Also, he was the lab manager for the normal operation of our lab.<br/>
 +
<b>YE Yixuan</b> was responsible for human practice and part of the construction of the mathematical model.<br/>
 +
<b>Zhang Xiaohan</b> was in charge of Conference of China iGEMer’s Committee and also helped in human practice and poster designing.
 +
</div>
 +
<h3 class="classic-title" id="Overview" style="margin-top:50px;"><span>Acknowledgement</span></h3>
 +
<p><b>Peking iGEM 2015 team highly acknowledges the following institutes:</b></p>
 +
<ul>
 +
<li>School of Life Sciences, Peking University</li><li>
 +
Office of Educational Administration, Peking University </li><li>
 +
Center for Quantitative Biology, Peking University </li><li>
 +
School of Physics, Peking University </li><li>
 +
School of Basic Medical Sciences, Peking University </li><li>
 +
Institute of Microbiology, Chinese Academy of Sciences </li><li>
 +
Beijing research institute for tuberculosis control</li>
 +
</ul>
 +
<p><b>We thank the following professors from Peking University for supporting our project with equipment, chemicals and computer programs:</b></p> 
 +
<ul>
 +
<li>Prof. OUYANG Qi</li><li>
 +
Prof. LOU Chubo</li><li>
 +
Prof. LAI Luhua</li><li>
 +
Prof. HE Xinqiang </li><li>
 +
Prof. XU Chongren</li>
 +
</ul>
  
  
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<div class="highlightBox">
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<p><big><b>Lab support</b></big></p>
 +
<ul>
 +
<li>
 +
We thank Dr. ZHAO Huiwei for providing us with the plasmids of dCas9 and linker(GGGGS*2) for dCas9 and luciferase connecting;</li><li>
 +
We thank Professor LAI Luhua for providing us with the pET21a plasmid;</li><li>
 +
We thank Dr. HOU Junran for providing us with the plasmid carrying FRB and FKBP protein;</li><li>
 +
We thank ZHANG Haoqian for providing us with the pSB1A3 plasmid with BsaI sites removement;</li><li>
 +
We thank Graduate LIU Ruicun from USTC for giving us the plasmid WJ024X containing DHFR;</li><li>
 +
We thank Dr. ZHAO Xuejin for giving us the plasmid containing LacZ and gRNA Generator plasmid, providing their lab for protein purification and providing technical supports and equipment supports;</li><li>
 +
We thank Dr. LIU Cuihua for providing us the isolated genomic of M.tuberculosis.</li><li>
 +
We highly appreciate the generous material assistance of Professor LOU Chunbo.</li>
 +
</ul>
 +
<p><big><b>Wiki support</b></big></p>
 +
<p>Our instructor, ZHANG Haoqian, gave us many ideas about the structure of wiki and the polishment of language.</p>
 +
<p><big><b>Presentation coaching</b></big></p>
 +
<ul>
 +
<li>
 +
Professor OUYANG Qi and Professor LOU Chunbo coached us for the presentation.</li><li>
 +
Professor LAI Luhua, Professor LI Fangting and Professor RAO Yi participated in our presentation rehearsal and made suggestions for revisions.</li>
 +
</ul>
 +
<p><big><b>Human practices support</b></big></p>
 +
<ul>
 +
<li>Doctor LI Bo from Beijing research institute for tuberculosis control was an invaluable resource for informing us on all aspects of TB facts.</li><li>
 +
Director DING Beichuan of Central Laboratory in Beijing research institute for tuberculosis control showed us around the advanced TB detecting laboratory and gave advice for our detecting system.</li><li>
 +
Director WANG Suming from Beijing research institute for tuberculosis control accepted our interview and gave advice for our project.</li><li>
 +
Doctor XIE Guangkuan from the Institute of Medical Humanities of Peking University accepted our interview and advised on the ethics discussion. </li><li>
 +
Director XIA Kebiao from Infectious Disease Department in Zhenghe County Hospital and Director LV Tingrong from Zhenghe Epidemic Prevention Station provided us with the epidemic situation of TB in grass-roots units.</li><li>
 +
Zhenghe No.1 Middle School of Fujian Province provided us a chance for advertising the basic knowledge of TB control and synthetic biology to the high school students.</li><li>
 +
ZHAO Mingyue, a freshman of Heilongjiang University, gave us much help in questionnaire survey, handing out propagandistic handbooks and maintaining the lab operation.</li>
 +
</ul>
 +
<p><big><b>Fundraising help and advice</b></big></p>
 +
<ul>
 +
<li>
 +
BluePHA® and Synbio Tech® supported the CCiC (.Conference of China iGEMer Committee) as sponsors.</li><li>
 +
Our project is funded by School of Life Sciences, Peking University.</li>
 +
</ul>
 +
<p><big><b>Last but not least</b></big></p>
 +
<ul>
 +
<li>
 +
We would like to thank all the people who came to our progress presentations and gave useful feedback and input.</li><li>
 +
We would like to thank all the 34 iGEM teams for participanting the CCiC and the volunteers for providing indispensable help. They really gave us lots of inspiration and suggestions.</li>
 +
</ul>
  
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<h4> Can we base our project on a previous one? </h4>
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                  </div>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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</div>
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<h4> Why is this page needed? </h4>
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                </div>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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            </div>
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<p>
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          </div>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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                            <ul  style="font-size:12px;color:#b4b4b4">
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                                <li><span>Email:</span> <a href="Mailto:pekingigem@gmail.com&amp;Subject=Hello&amp;Body=Hi, Peking iGEM!"style="color:#b4b4b4">pekingigem@gmail.com</li>
 +
                                <li><span>Website:</span> <a href="2015.igem.org/Team:Peking" style="color:#b4b4b4"> 2015.igem.org/Team:Peking</a></li>
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                                <li><span>Address:</span> Peking University, <br>No.5 Yiheyuan Road Haidian District, <br>Beijing, P.R.China 100871</li>
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<h5> What should this page have?</h5>
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                            <p class="text-center">&copy; 2015 Peking iGEM -  All Rights Reserved. Based on <a href="http://getbootstrap.com/"style="color:#b4b4b4">Bootstrap</a> and Margo template.</p>
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<ul>
 
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<li>General Support</li>
 
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<li>Project support and advice</li>
 
−
<li>Fundraising help and advice</li>
 
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<li>Lab support</li>
 
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<li>Difficult technique support</li>
 
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<li>Project advisor support</li>
 
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<li>Wiki support</li>
 
−
<li>Presentation coaching</li>
 
−
<li>Human Practices support</li>
 
−
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
 
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</ul>
 
  
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−
<h4>Inspiration</h4>
 
−
<p>Take a look at what other teams have done:</p>
 
−
<ul>
 
−
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
−
</ul>
 
  
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</div>
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</body>
 
</html>
 
</html>

Revision as of 16:09, 18 September 2015

Peking iGEM 2015

Safety

Better to be safe than sorry.

Literature Investigation and Economical Assessment

Member’s Attributions

ZHANG Yihao is the team leader of 2015 Peking iGEM team. He participated in designing of the project, took charge of troubleshooting, and arranged the experimental schedule, making sure that the whole lab function smoothly and head in the right direction.
WEI Weijia committed to ameliorate the detection system, that's to say, to enhance the sensitivity as well as to reduce the difficulty of detection. He was also in responsible for gathering plasmid information and seeking appropriate substrates.
LI Hang was responsible for designing and manufacturing our devices, which are for detection of analogue electrical signal and optical signal.
CHENG Sida was in charge of Human Practice and also works in the wet lab to construct and prepare plasmids for variable gRNA in vitro transcription and fusion protein construction.
LI Jiaofeng worked as the financial manager of the team. She was also responsible for parts construction and submission.
LI Xiang was in charge of constructing another kind of M.tuberculosis sensing system using Molecule Beacon. He was also engaged in the protein purification of dCas9 fusion protein.
LING Pingping was in charge of testing the luminescence intensity of split firefly luciferase fused with CRISPR system. She also processed the whole experimental data.
LIU Dongming helped to accomplish the plasmid construction of the detecting target and was responsible for human practice.
LIU Wenchao was in charge of construction of CRISPR gRNA as well as RNA scaffold at the beginning. Lately she was working on protein purification of dCas9 fusion protein.
WANG Beining undertook the task of cloning for the dCas9-luciferase part. She was also responsible for the testing of CRISPR-Luciferase system.
WANG Yu participated in plasmid construction initially, protein purification, and wiki designing and building. She was also the designer of our PowerPoint.
XU Luze was in charge of Modelling. His main work was programmed to search for the suitable guide sequences. Also, he was the lab manager for the normal operation of our lab.
YE Yixuan was responsible for human practice and part of the construction of the mathematical model.
Zhang Xiaohan was in charge of Conference of China iGEMer’s Committee and also helped in human practice and poster designing.

Acknowledgement

Peking iGEM 2015 team highly acknowledges the following institutes:

  • School of Life Sciences, Peking University
  • Office of Educational Administration, Peking University
  • Center for Quantitative Biology, Peking University
  • School of Physics, Peking University
  • School of Basic Medical Sciences, Peking University
  • Institute of Microbiology, Chinese Academy of Sciences
  • Beijing research institute for tuberculosis control

We thank the following professors from Peking University for supporting our project with equipment, chemicals and computer programs:

  • Prof. OUYANG Qi
  • Prof. LOU Chubo
  • Prof. LAI Luhua
  • Prof. HE Xinqiang
  • Prof. XU Chongren

Lab support

  • We thank Dr. ZHAO Huiwei for providing us with the plasmids of dCas9 and linker(GGGGS*2) for dCas9 and luciferase connecting;
  • We thank Professor LAI Luhua for providing us with the pET21a plasmid;
  • We thank Dr. HOU Junran for providing us with the plasmid carrying FRB and FKBP protein;
  • We thank ZHANG Haoqian for providing us with the pSB1A3 plasmid with BsaI sites removement;
  • We thank Graduate LIU Ruicun from USTC for giving us the plasmid WJ024X containing DHFR;
  • We thank Dr. ZHAO Xuejin for giving us the plasmid containing LacZ and gRNA Generator plasmid, providing their lab for protein purification and providing technical supports and equipment supports;
  • We thank Dr. LIU Cuihua for providing us the isolated genomic of M.tuberculosis.
  • We highly appreciate the generous material assistance of Professor LOU Chunbo.

Wiki support

Our instructor, ZHANG Haoqian, gave us many ideas about the structure of wiki and the polishment of language.

Presentation coaching

  • Professor OUYANG Qi and Professor LOU Chunbo coached us for the presentation.
  • Professor LAI Luhua, Professor LI Fangting and Professor RAO Yi participated in our presentation rehearsal and made suggestions for revisions.

Human practices support

  • Doctor LI Bo from Beijing research institute for tuberculosis control was an invaluable resource for informing us on all aspects of TB facts.
  • Director DING Beichuan of Central Laboratory in Beijing research institute for tuberculosis control showed us around the advanced TB detecting laboratory and gave advice for our detecting system.
  • Director WANG Suming from Beijing research institute for tuberculosis control accepted our interview and gave advice for our project.
  • Doctor XIE Guangkuan from the Institute of Medical Humanities of Peking University accepted our interview and advised on the ethics discussion.
  • Director XIA Kebiao from Infectious Disease Department in Zhenghe County Hospital and Director LV Tingrong from Zhenghe Epidemic Prevention Station provided us with the epidemic situation of TB in grass-roots units.
  • Zhenghe No.1 Middle School of Fujian Province provided us a chance for advertising the basic knowledge of TB control and synthetic biology to the high school students.
  • ZHAO Mingyue, a freshman of Heilongjiang University, gave us much help in questionnaire survey, handing out propagandistic handbooks and maintaining the lab operation.

Fundraising help and advice

  • BluePHA® and Synbio Tech® supported the CCiC (.Conference of China iGEMer Committee) as sponsors.
  • Our project is funded by School of Life Sciences, Peking University.

Last but not least

  • We would like to thank all the people who came to our progress presentations and gave useful feedback and input.
  • We would like to thank all the 34 iGEM teams for participanting the CCiC and the volunteers for providing indispensable help. They really gave us lots of inspiration and suggestions.