Difference between revisions of "Team:Hong Kong-CUHK/insertion kit"

Line 21: Line 21:
  
  
−
<h4><b><u><font face="Times New Roman" size="5pt">The insertion kit: </font></u></b></h4>
+
<font color=#ff0000>vector map!!!</font>
  
−
<center><img src="https://static.igem.org/mediawiki/2015/7/7a/CUHK_Project_MamC.jpg" width ="500px" style="right: 10px; margin:-30px 0px -70px -10px"></center>
+
<h1>Insertion Kit for Protein Expression on Magnetosome Membrane</h1>
−
<p>Figure 1: The structure of the trans-membrane MamC protein</p>
+
  
−
<p><font face="Times New Roman" size="4pt">This construct is actually a simple one, consisting of a mamC gene, a gene coding for a trans-membrane protein (1) (Figure 1) on the magnetosome membrane, in a vector. However, unlike usual recombinant methods in which we put our insert between the multiple restriction sites, we are putting our mamC gene in front of it. Through this method, it enables is to attach any protein we desired on the magnetosome membrane just by fusing it with the mamC gene by inserting it between the multiple restriction sites. (For your interest, this is done by removing the stop codon of the mamC gene and the start codon of the desired protein, for example an antibody, making it a mamC fused protein). </p></font>
+
<center><img src="https://static.igem.org/mediawiki/2015/7/7a/CUHK_Project_MamC.jpg" width ="500px" style="right: 10px; margin:0px 0px -70px -10px"></center>
 +
<p>Figure 1: The structure of the transmembrane mamC protein</p>
  
−
<p><font face="Times New Roman" size="4pt">As we are now putting multiple restriction sites behind mamC, therefore we can insert any desire genes afterwards. Thus, we name it our insertion kit.</p></font>
+
<p>This simple construct consists of mamC gene, a gene coding for a transmembrane protein (1) (Figure 1) on the magnetosome membrane. Unlike usual recombinant methods to put our insert between multiple restriction sites, we put mamC in front of them. Now any protein we desired can be attached onto the magnetosome membrane just by fusing it with the mamC gene <b>by inserting it between the multiple restriction sites</b>.</p><p>(For your interest, this is done by removing the stop codon of mamC and the start codon of the desired protein, for example an antibody, making it a mamC-fused protein). </p>
 +
 
 +
<p>As we are now putting multiple restriction sites behind mamC, therefore we can insert any desire genes afterwards. Thus, we name it our insertion kit.</p>
 +
<center><h1><b>Magnetosome + Insertion Kit = Multi-application !!!</b></h1></center>
 
<p>
 
<p>
 
1.XU, Jun, et al. Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes. Frontiers in microbiology, 2014, 5.
 
1.XU, Jun, et al. Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes. Frontiers in microbiology, 2014, 5.
 
</p>
 
</p>
−
<center><h1><b>Magnetosome and the insertion kit = multi-application !</b></h1></center>
 
−
 
 
</center>
 
</center>
  

Revision as of 17:04, 18 September 2015

vector map!!!

Insertion Kit for Protein Expression on Magnetosome Membrane

Figure 1: The structure of the transmembrane mamC protein

This simple construct consists of mamC gene, a gene coding for a transmembrane protein (1) (Figure 1) on the magnetosome membrane. Unlike usual recombinant methods to put our insert between multiple restriction sites, we put mamC in front of them. Now any protein we desired can be attached onto the magnetosome membrane just by fusing it with the mamC gene by inserting it between the multiple restriction sites.

(For your interest, this is done by removing the stop codon of mamC and the start codon of the desired protein, for example an antibody, making it a mamC-fused protein).

As we are now putting multiple restriction sites behind mamC, therefore we can insert any desire genes afterwards. Thus, we name it our insertion kit.

Magnetosome + Insertion Kit = Multi-application !!!

1.XU, Jun, et al. Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes. Frontiers in microbiology, 2014, 5.