Difference between revisions of "Team:Hong Kong-CUHK/Parts"

Line 70: Line 70:
 
     <td>BBa_K1648045</td>
 
     <td>BBa_K1648045</td>
 
     <td></td>
 
     <td></td>
−
     <td>oprF from Azotobacter vinelandii</td>
+
     <td>oprF from <i>Azotobacter vinelandii</i></td>
 
     <td>Allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>Allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>In pSB1C3 (submitted)</td>
 
     <td>In pSB1C3 (submitted)</td>
Line 77: Line 77:
 
     <td>BBa_K1648046</td>
 
     <td>BBa_K1648046</td>
 
     <td></td>
 
     <td></td>
−
     <td>Mutated oprF from Azotobacter vinelandii with five-point mutations</td>
+
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations</td>
 
     <td>Give 5-fold transmission efficiency than original oprF</td>
 
     <td>Give 5-fold transmission efficiency than original oprF</td>
 
     <td>Planing</td>
 
     <td>Planing</td>
Line 83: Line 83:
 
     <td>BBa_K1648047</td>
 
     <td>BBa_K1648047</td>
 
     <td></td>
 
     <td></td>
−
     <td>oprF from Azotobacter vinelandii with RBS</td>
+
     <td>oprF from <i>Azotobacter vinelandii</i> with RBS</td>
 
     <td>Intermediate part for transcription of oprF</td>
 
     <td>Intermediate part for transcription of oprF</td>
 
     <td>Planing</td>
 
     <td>Planing</td>
Line 90: Line 90:
 
     <td>BBa_K1648048</td>
 
     <td>BBa_K1648048</td>
 
     <td></td>
 
     <td></td>
−
     <td>oprF from Azotobacter vinelandii promoted by tetRpro</td>
+
     <td>oprF from <i>Azotobacter vinelandii</i> promoted by tetRpro</td>
 
     <td>Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>In pSB1A3 (submitted)</td>
 
     <td>In pSB1A3 (submitted)</td>
Line 97: Line 97:
 
     <td>BBa_K1648049</td>
 
     <td>BBa_K1648049</td>
 
     <td></td>
 
     <td></td>
−
     <td>Mutated oprF from Azotobacter vinelandii with five-point mutations promoted by tetRpro</td>
+
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations promoted by tetRpro</td>
 
     <td>Constitutive production of mutated oprF</td>
 
     <td>Constitutive production of mutated oprF</td>
 
     <td>In pSB1C3 (submitted)</td>
 
     <td>In pSB1C3 (submitted)</td>
Line 103: Line 103:
 
     <td>BBa_K1648050</td>
 
     <td>BBa_K1648050</td>
 
     <td></td>
 
     <td></td>
−
     <td>suspecting porin from Pseudomonas fluorescens with constitutive promoter </td>
+
     <td>suspecting porin from <i>Pseudomonas fluorescens</i> with constitutive promoter </td>
 
     <td>Expression of oprF for characterization</td>
 
     <td>Expression of oprF for characterization</td>
 
     <td>In pSB1C3 (submitted)</td>
 
     <td>In pSB1C3 (submitted)</td>
Line 110: Line 110:
 
     <td>BBa_K1648051</td>
 
     <td>BBa_K1648051</td>
 
     <td></td>
 
     <td></td>
−
     <td>Forming porin on Azotobacter vinelandii and templates for overexpressing hydrogenase in Azotobacter vinelandii</td>
+
     <td>Forming porin on <i>Azotobacter vinelandii</i> and templates for overexpressing hydrogenase in Azotobacter vinelandii</td>
−
     <td>overexpress both porin and hydrogenase once transformed into Azotobacter vinelandii with homologous recombination</td>
+
     <td>overexpress both porin and hydrogenase once transformed into <i>Azotobacter vinelandii</i> with homologous recombination</td>
 
     <td>In pSB1C3 (submitted)</td>
 
     <td>In pSB1C3 (submitted)</td>
 
</tr>
 
</tr>

Revision as of 18:29, 18 September 2015

An important aspect of the iGEM competition is the use and creation of standard biological parts.

Here is a table of summary of the parts that we constructed or have submitted to the registry

Name Natural function of part Usage Copies
BBa_K1648000 Basic Part flanking sequence of mamHIEJKLMN from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon In pSB1C3 (submitted)
BBa_K1648001 Basic Part flanking sequence of mamOPAQRBSTU from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons (not submitted)
BBa_K1648002 Composite part Biobrick for homologous recombination with mamAB operon from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon(contains a promotor and terminator) In pSB1A3 (stocked) In pSB1C3 (submitted)
BBa_K1648003 Composite part Biobrick for homologous recombination with mamGC, mamXY, mms operons from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons(contains a promotor and terminator) In pSB1C3 (submitted)
BBa_K1648004 Basic Part Biobrick for mamC fuse protein on magnetosome membrane Template for fuse protein of interest to magnetosome membrane In pSB1C3 (submitted)
BBa_K1648005 Basic Part Functional biobrick for GFP nanobody Express GFP nanobody to conduct immunoprecipitation with GFP In pSB1C3 (submitted)
BBa_K1648006 Composite part Functional biobrick for mamC fuse protein on magnetosome membrane and GFP nanobody fuse GFP nanobody to magnetosome membrane In pSB1C3 (submitted)
BBa_K1648045 oprF from Azotobacter vinelandii Allows non-specific transport from bacteria and extracellular environment In pSB1C3 (submitted)
BBa_K1648046 Mutated oprF from Azotobacter vinelandii with five-point mutations Give 5-fold transmission efficiency than original oprF Planing
BBa_K1648047 oprF from Azotobacter vinelandii with RBS Intermediate part for transcription of oprF Planing
BBa_K1648048 oprF from Azotobacter vinelandii promoted by tetRpro Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment In pSB1A3 (submitted)
BBa_K1648049 Mutated oprF from Azotobacter vinelandii with five-point mutations promoted by tetRpro Constitutive production of mutated oprF In pSB1C3 (submitted)
BBa_K1648050 suspecting porin from Pseudomonas fluorescens with constitutive promoter Expression of oprF for characterization In pSB1C3 (submitted)
BBa_K1648051 Forming porin on Azotobacter vinelandii and templates for overexpressing hydrogenase in Azotobacter vinelandii overexpress both porin and hydrogenase once transformed into Azotobacter vinelandii with homologous recombination In pSB1C3 (submitted)