Difference between revisions of "Team:Hong Kong-CUHK/Parts"

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<tr>
 
<tr>
 
     <th>Name</th>
 
     <th>Name</th>
−
     <th></th>
+
     <th>Basic/Composite</th>
 
     <th>Natural function of part</th>
 
     <th>Natural function of part</th>
 
     <th>Usage</th>
 
     <th>Usage</th>
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<tr>
 
<tr>
 
     <td>BBa_K1648045</td>
 
     <td>BBa_K1648045</td>
−
     <td></td>
+
     <td>Basic</td>
 
     <td>oprF from <i>Azotobacter vinelandii</i></td>
 
     <td>oprF from <i>Azotobacter vinelandii</i></td>
 
     <td>Allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>Allows non-specific transport from bacteria and extracellular environment</td>
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<tr>
 
<tr>
 
     <td>BBa_K1648046</td>
 
     <td>BBa_K1648046</td>
−
     <td></td>
+
     <td>Basic</td>
 
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations</td>
 
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations</td>
 
     <td>Give 5-fold transmission efficiency than original oprF</td>
 
     <td>Give 5-fold transmission efficiency than original oprF</td>
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</tr><tr>
 
</tr><tr>
 
     <td>BBa_K1648047</td>
 
     <td>BBa_K1648047</td>
−
     <td></td>
+
     <td>Composite</td>
 
     <td>oprF from <i>Azotobacter vinelandii</i> with RBS</td>
 
     <td>oprF from <i>Azotobacter vinelandii</i> with RBS</td>
 
     <td>Intermediate part for transcription of oprF</td>
 
     <td>Intermediate part for transcription of oprF</td>
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<tr>
 
<tr>
 
     <td>BBa_K1648048</td>
 
     <td>BBa_K1648048</td>
−
     <td></td>
+
     <td>Composite</td>
 
     <td>oprF from <i>Azotobacter vinelandii</i> promoted by tetRpro</td>
 
     <td>oprF from <i>Azotobacter vinelandii</i> promoted by tetRpro</td>
 
     <td>Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment</td>
 
     <td>Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment</td>
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<tr>
 
<tr>
 
     <td>BBa_K1648049</td>
 
     <td>BBa_K1648049</td>
−
     <td></td>
+
     <td>Composite</td>
 
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations promoted by tetRpro</td>
 
     <td>Mutated oprF from <i>Azotobacter vinelandii</i> with five-point mutations promoted by tetRpro</td>
 
     <td>Constitutive production of mutated oprF</td>
 
     <td>Constitutive production of mutated oprF</td>
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</tr><tr>
 
</tr><tr>
 
     <td>BBa_K1648050</td>
 
     <td>BBa_K1648050</td>
−
     <td></td>
+
     <td>Basic</td>
 
     <td>suspecting porin from <i>Pseudomonas fluorescens</i> with constitutive promoter </td>
 
     <td>suspecting porin from <i>Pseudomonas fluorescens</i> with constitutive promoter </td>
 
     <td>Expression of oprF for characterization</td>
 
     <td>Expression of oprF for characterization</td>
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<tr>
 
<tr>
 
     <td>BBa_K1648051</td>
 
     <td>BBa_K1648051</td>
−
     <td></td>
+
     <td>Basic</td>
 
     <td>Forming porin on <i>Azotobacter vinelandii</i> and templates for overexpressing hydrogenase in <i>Azotobacter vinelandii</i></td>
 
     <td>Forming porin on <i>Azotobacter vinelandii</i> and templates for overexpressing hydrogenase in <i>Azotobacter vinelandii</i></td>
 
     <td>overexpress both porin and hydrogenase once transformed into <i>Azotobacter vinelandii</i> with homologous recombination</td>
 
     <td>overexpress both porin and hydrogenase once transformed into <i>Azotobacter vinelandii</i> with homologous recombination</td>

Revision as of 18:49, 18 September 2015

An important aspect of the iGEM competition is the use and creation of standard biological parts.

Here is a table of summary of the parts that we constructed or have submitted to the registry

Name Basic/Composite Natural function of part Usage Copies
BBa_K1648000 Basic Part flanking sequence of mamHIEJKLMN from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon In pSB1C3 (submitted)
BBa_K1648001 Basic Part flanking sequence of mamOPAQRBSTU from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons (not submitted)
BBa_K1648002 Composite part Biobrick for homologous recombination with mamAB operon from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon(contains a promotor and terminator) In pSB1A3 (stocked) In pSB1C3 (submitted)
BBa_K1648003 Composite part Biobrick for homologous recombination with mamGC, mamXY, mms operons from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons(contains a promotor and terminator) In pSB1C3 (submitted)
BBa_K1648004 Basic Part Biobrick for mamC fuse protein on magnetosome membrane Template for fuse protein of interest to magnetosome membrane In pSB1C3 (submitted)
BBa_K1648005 Basic Part Functional biobrick for GFP nanobody Express GFP nanobody to conduct immunoprecipitation with GFP In pSB1C3 (submitted)
BBa_K1648006 Composite part Functional biobrick for mamC fuse protein on magnetosome membrane and GFP nanobody fuse GFP nanobody to magnetosome membrane In pSB1C3 (submitted)
BBa_K1648045 Basic oprF from Azotobacter vinelandii Allows non-specific transport from bacteria and extracellular environment In pSB1C3 (submitted)
BBa_K1648046 Basic Mutated oprF from Azotobacter vinelandii with five-point mutations Give 5-fold transmission efficiency than original oprF Planing
BBa_K1648047 Composite oprF from Azotobacter vinelandii with RBS Intermediate part for transcription of oprF Planing
BBa_K1648048 Composite oprF from Azotobacter vinelandii promoted by tetRpro Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment In pSB1A3 (submitted)
BBa_K1648049 Composite Mutated oprF from Azotobacter vinelandii with five-point mutations promoted by tetRpro Constitutive production of mutated oprF In pSB1C3 (submitted)
BBa_K1648050 Basic suspecting porin from Pseudomonas fluorescens with constitutive promoter Expression of oprF for characterization In pSB1C3 (submitted)
BBa_K1648051 Basic Forming porin on Azotobacter vinelandii and templates for overexpressing hydrogenase in Azotobacter vinelandii overexpress both porin and hydrogenase once transformed into Azotobacter vinelandii with homologous recombination In pSB1C3 (submitted)