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| − | <p class=MsoNormal><b style='mso-bidi-font-weight:normal'><span lang=EN-US
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| − | style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Overview<o:p></o:p></span></b></p>
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| − |
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| − | <p class=MsoNormal><span lang=EN-US style='font-size:15.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'>Background</span><b
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| − | style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:16.0pt;
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| − | font-family:"Arial Unicode MS",sans-serif'><o:p></o:p></span></b></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>In 1930s, a new, strange disease happened in
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| − | Toyama village, Japan, called Itai-Itai disease, whose symptoms were exactly
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| − | the same as it was. Patients had insufferable pains in bones. The whole body
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| − | anywhere was very easy to </span><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;background:
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| − | #FEFEFE'>fracture<span style='color:#333333'>. They had been shouting “Pain!
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| − | Pain!” until death. Now we know, the cause of the disease is cadmium poisoning.
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| − | The river that local people used for drinking and irrigating crops was polluted
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| − | by the sewage, containing a large amount of cadmium. It was discharged from the
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| − | Zinc smelting plant, which was built on the upper reaches of the river. Cadmium
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| − | through food chain gradually enriched in human body. Up to now, in 2014, a
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| − | village in Guangxi, China also has the occurrence of Itai-Itai disease.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=center style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:center;text-indent:24.0pt'><span
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| − | lang=EN-US style='font-family:"Arial Unicode MS",sans-serif'><v:rect id="rectole0000000000"
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| − | o:spid="_x0000_i1025" style='width:159pt;height:303pt' o:ole=""
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| − | o:preferrelative="t" stroked="f">
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| − | <a href="https://static.igem.org/mediawiki/parts/2/2c/2015SCUT-O-1.jpg" rel="prettyPhoto[pp_gal]">
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| − | <span class="item-on-hover"><span class="hover-image"></span></span>
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| − | <img src="https://static.igem.org/mediawiki/parts/2/2c/2015SCUT-O-1.jpg" alt=" " />
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| − | </a>
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| − | </v:rect><!--[if gte mso 9]><xml>
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| − | <o:OLEObject Type="Embed" ProgID="StaticMetafile" ShapeID="rectole0000000000"
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| − | DrawAspect="Content" ObjectID="_1504136495">
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| − | </xml><![endif]--></span><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=center style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:center;text-indent:24.0pt;
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| − | line-height:137%'><span lang=EN-US style='font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>Figure1: Patients with bone deformation due
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| − | to cadmium poisoning<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:120.75pt;
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| − | mso-char-indent-count:11.5;line-height:137%'><span lang=EN-US style='font-family:
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| − | style='width:398.25pt;height:599.25pt' o:ole="" o:preferrelative="t"
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| − | stroked="f">
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| − | <v:imagedata src="background%20&v%20overview.files/image002.png" o:title=""/>
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| − | </v:rect><!--[if gte mso 9]><xml>
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| − | </o:OLEObject>
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| − | </xml><![endif]--><span style='color:#333333;background:#FEFEFE'><o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:108.0pt;
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| − | mso-char-indent-count:9.0'><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'>Figure
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| − | 2: A child was moaning because the great pain caused by Itai-Itai disease.<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:24.0pt'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>Cadmium’s entering human body can make it a
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| − | huge loss of calcium in bones and trigger a series of damage, like
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| − | osteoporosis, bone atrophy, joint pain and so on. Once had a patient, hit a
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| − | sneeze, unexpectedly leading to several fracture. Even mild cadmium poisoning
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| − | can cause lung and kidney damage, like toxic pulmonary edema and chemical pneumonia.<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:24.0pt'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:189.0pt;
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| − | mso-char-indent-count:18.0'><span lang=EN-US style='font-family:"Arial Unicode MS",sans-serif'><v:rect
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| − | id="rectole0000000002" o:spid="_x0000_i1027" style='width:318pt;height:159pt'
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| − | o:ole="" o:preferrelative="t" stroked="f">
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| − | <v:imagedata src="background%20&v%20overview.files/image003.png" o:title=""/>
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| − | </v:rect><!--[if gte mso 9]><xml>
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| − | <o:OLEObject Type="Embed" ProgID="StaticMetafile" ShapeID="rectole0000000002"
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| − | DrawAspect="Content" ObjectID="_1504136498">
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| − | </o:OLEObject>
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| − | </xml><![endif]--></span><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'><span
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| − | style='mso-spacerun:yes'> </span><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:216.0pt;
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| − | mso-char-indent-count:18.0'><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;
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| − | background:#FEFEFE'>Figure 3:<span style='mso-spacerun:yes'>
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| − | </span>Cadmium does harm to the bone seriously.<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>Asia is the world's largest primary cadmium
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| − | metal producing areas, mainly in </span><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;background:
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| − | #FEFEFE'>China, South Korea, and Japan. But industry that is related to cadmium
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| − | is globally existed</span><span style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;background:#FEFEFE'>：<span
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| − | lang=EN-US>Smelting, spraying, welding and casting bearing surface of cadmium</span>；<span
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| − | lang=EN-US>Stope</span>；<span lang=EN-US>Rods in nuclear reactors</span>；<span
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| − | lang=EN-US>Neutron absorber</span>；<span lang=EN-US>Electroplating factory, and
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| − | so on. <o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | background:#FEFEFE'>So, do you<span style='color:#333333'> think cadmium is far
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| − | away from our daily life? No.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>In June 2010, the U.S. Consumer Product
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| − | Safety Commission confirmed that the glasses sold by McDonald were found to
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| − | contain cadmium elements in the </span><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#313131;
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| − | background:#FEFEFE'>paintcoat</span><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;
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| − | background:#FEFEFE'>, which could exude from the surface of the glasses. About
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| − | twelve million glasses were recalled in the United States.<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:173.25pt;
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| − | mso-char-indent-count:16.5'><span lang=EN-US style='font-family:"Arial Unicode MS",sans-serif'><v:rect
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| − | o:ole="" o:preferrelative="t" stroked="f">
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| − | <v:imagedata src="background%20&v%20overview.files/image004.png" o:title=""/>
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| − | </v:rect><!--[if gte mso 9]><xml>
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| − | </xml><![endif]--></span><span lang=EN-US style='font-size:0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:black;background:black'><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left;text-indent:120.75pt;
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| − | mso-char-indent-count:11.5'><span lang=EN-US style='font-family:"Arial Unicode MS",sans-serif'>Figure
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| − | <span style='mso-spacerun:yes'> </span>4 </span><span style='font-family:
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| − | "Arial Unicode MS",sans-serif'>：<span lang=EN-US>The <span style='color:#313131'>paintcoat
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| − | of the glasses sold by McDonald were detected to contain cadmium elements </span><o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>In 2012</span><span style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;
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| − | background:#FEFEFE'>，<span lang=EN-US>the report from the environmental
| |
| − | organization of Canada about Potential Damage of Heavy Metals in Cosmetics
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| − | said, almost all of the tested cosmetics contain toxic substances, including
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| − | cadmium.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>In June 2012</span><span style='font-size:
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| − | 12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>，<span lang=EN-US>Shandong Province, Rizhao
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| − | City inspection and Quarantine Bureau<span style='mso-spacerun:yes'>
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| − | </span>seized 24 tons of heavy sauries from Japan , whose Cd content exceeded
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| − | the safety standard.<o:p></o:p></span></span></p>
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| − |
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| − | margin-left:0cm;text-indent:99.75pt;mso-char-indent-count:9.5'><span
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| − | <v:stroke joinstyle="miter"/>
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| − | <v:formulas>
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| − | <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
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| − | cropbottom="2131f"/>
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| − | </v:shape></span><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;line-height:137%'><span
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| − | lang=EN-US style='font-family:"Arial Unicode MS",sans-serif;background:#FEFEFE'>Figure
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| − | 5</span><span style='font-family:"Arial Unicode MS",sans-serif;background:#FEFEFE'>：<span
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| − | lang=EN-US>Rizhao(China) inspection and quarantine bureau staff at the time of
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| − | sampling inspection, found that cadmium content of these 24tons saury exceeded
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| − | the safety standard.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:18.0pt'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>Smoking 20 cigarettes a day, 2 to 4
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| − | micrograms of cadmium can be taken in <o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:168.0pt;
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| − | mso-char-indent-count:16.0'><span lang=EN-US style='font-family:"Arial Unicode MS",sans-serif'><v:rect
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| − | </v:rect><!--[if gte mso 9]><xml>
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| − | </xml><![endif]--></span><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | margin-bottom:3.75pt;margin-left:0cm;text-align:left;text-indent:204.75pt;
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| − | mso-char-indent-count:19.5;line-height:137%'><span lang=EN-US style='font-family:
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| − | "Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'>Figure 6</span><span
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| − | style='font-family:"Arial Unicode MS",sans-serif;color:#333333;background:#FEFEFE'>：<span
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| − | lang=EN-US>Cadmium containing rice due to water and soil pollution.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;margin-right:0cm;
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-top:5.0pt;text-align:left'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333;background:#FEFEFE'>Since there is no effective treatment of Itai
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| − | Itai disease, and cadmium stored in the body did not rule out in a safe and
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| − | effective method. Therefore, it is especially important to eliminate cadmium
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| − | pollution on the environment; this is the fundamental measure to prevent the
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| − | occurrence of pain!<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | color:#333333'>So we got the idea of our project: Super cadmium ion killer<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left;text-indent:18.0pt'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:16.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>Project
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| − | Overview<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='mso-bidi-font-size:10.5pt;font-family:"Arial Unicode MS",sans-serif'>Concerning
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| − | cadmium pollution,<span style='background:white'> the iGEM team 2015 SCUT would
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| − | like to present super cadmium ion killer, a bacterial platform designed to
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| − | detect and absorb the cadmium in sewage all over the world. We employ </span>E.coli
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| − | superficial fiber curli protein—CsgA and synthetic phytochelatins (ECs) to form
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| − | CsgA-ECs, which can effectively remove cadmium ion in wastewater.<span
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| − | style='color:#3A3424;background:white'> </span><span style='background:
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| − | white'>Detection of cadmium ion presence in the sewage and
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| − | indication the concentration with various colors form the first part of the
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| − | super killer system. Expression of CsgA-ECs coupled to the sensor for
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| − | cadmium ion forms the second part of the super killer system. When our E.coli is
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| − | in a certain condition of low cadmium ion concentration, only the <span
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| − | style='mso-bidi-font-weight:bold'>green chromoprotein</span> cjBlue will be
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| − | expressed, leading to a color of green in culture. On the contrary, the
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| − | organism is on red alert with high concentration cadmium ion, <span
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| − | style='mso-bidi-font-weight:bold'>producing a red fluorescent protein RFP<span
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| − | style='color:#282828'> </span></span>as well as CsgA-EC(s) to absorb the ion.
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| − | It would be carried out until the concentration of cadmium ion dropping to a
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| − | low level. And the organism comes back to the origin state, the color return to
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| − | green again. As described above, our super cadmium ion killer system is
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| − | efficient, economical and real-time monitoring, which has great potential
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| − | application value in the treatment of cadmium pollution in the environment.<o:p></o:p></span></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left;text-indent:144.0pt;
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| − | mso-char-indent-count:12.0'><span lang=EN-US style='font-size:12.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif;mso-no-proof:
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| − | yes'><v:shape id="图片_x0020_7" o:spid="_x0000_i1030" type="#_x0000_t75" style='width:412.5pt;
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| − | height:300pt;visibility:visible;mso-wrap-style:square'>
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| − | <v:imagedata src="background%20&v%20overview.files/image007.gif" o:title="2015SCUT-O-7"/>
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| − | <o:lock v:ext="edit" cropping="t"/>
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| − | </v:shape></span><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:
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| − | 11.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>Figure
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| − | 7: Super cadmium ion killer absorbing process. With the presence of cadmium
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| − | ion, the organism expresses CsgA-ECs to absorb the ion and turns red from
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| − | green. <o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:16.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>Brief
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| − | introduction<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left;text-indent:18.0pt'><span
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| − | lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p> </o:p></span></p>
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| − |
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| − | <p class=MsoListParagraph align=left style='margin-left:21.0pt;text-align:left;
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| − | text-indent:-21.0pt;mso-char-indent-count:0;mso-list:l2 level1 lfo3'><![if !supportLists]><span
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| − | lang=EN-US style='font-size:14.0pt;mso-bidi-font-size:11.0pt;font-family:Wingdings;
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| − | mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings'><span
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| − | style='mso-list:Ignore'>l<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span
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| − | lang=EN-US style='font-size:14.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>cadmium
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| − | killing<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
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| − | style='font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>Cuili
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| − | fibers are powerful amyloid protein nano fibers, with a radius about 4-7 nm,
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| − | which are located in the surface of bacteria and expand as floccules. Assembled
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| − | by 13KDa CsgA extracellularly, Curli are ideal gene engineering application
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| − | platforms for extracellular absorption. Synthetic phytochelatins (EC) are naturally
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| − | occurring thiol-rich peptides with primary structures (Glu-Cys) n-Gly (n: repeat
| |
| − | unit), effectively binding Cd<sup>2+</sup> and Hg<sup>+</sup>. Based on the
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| − | features described above, our project aim at assemble a device for removing
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| − | cadmium by fusing CsgA and ECs to achieve absorbing Cd<sup>2+ </sup>extracellularly.
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| − | <o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='margin-left:0cm;text-align:left;
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| − | text-indent:0cm;mso-list:l1 level1 lfo2'><![if !supportLists]><span lang=EN-US
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| − | style='font-size:14.0pt;mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'><span
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| − | style='mso-list:Ignore'>•<span style='font:7.0pt "Times New Roman"'>
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| − | </span></span></span><![endif]><span lang=EN-US style='font-size:14.0pt;
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| − | mso-bidi-font-size:11.0pt;font-family:"Arial Unicode MS",sans-serif'>Cd<sup>2+</sup>
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| − | monitoring (sensor system)<o:p></o:p></span></p>
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| − |
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| − | <p class=MsoNormal align=left style='text-align:left;mso-layout-grid-align:
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| − | none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;font-family:
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| − | "Arial Unicode MS",sans-serif;mso-font-kerning:0pt;mso-bidi-font-weight:bold'>The
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| − | mercury-sensing regulatory protein, MerR, which regulates mercury resistance
| |
| − | operons in Gram-negative bacteria, was subjected to directed evolution in an
| |
| − | effort to generate a MerR mutant that responds to Cd but not Hg. </span><span
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| − | lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS",sans-serif;
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| − | mso-font-kerning:0pt'>MerR binds cadmium ion,<span style='mso-bidi-font-weight:
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| − | bold'> when it presences in the culture</span>. This provokes an allosteric
| |
| − | change in the MerR protein that triggers unwinding of the spacer DNA, allowing
| |
| − | transcription initiation of the <i>mer </i>operon,<span style='mso-bidi-font-weight:
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| − | bold'> which regulates the color of the culture and expression of CsgA-ECs.</span><o:p></o:p></span></p>
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| − |
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| − |
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| − |
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| − |
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| − | <div class="space"></div>
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| − |
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| − | <h6>PROJECT PHOTOS</h6>
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| − |
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| − |
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| − | <div class="portfolio-gallery">
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| − | <a href="images/portfolio/1_jj.jpg" rel="prettyPhoto[pp_gal]">
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| − | <span class="item-on-hover"><span class="hover-image"></span></span>
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| − | <img src="images/portfolio/1_jj.jpg" alt=" " />
| |
| − | </a>
| |
| − | <a href="images/portfolio/4_jj.jpg" rel="prettyPhoto[pp_gal]">
| |
| − | <span class="item-on-hover"><span class="hover-image"></span></span>
| |
| − | <img src="images/portfolio/4_jj.jpg" alt=" " />
| |
| − | </a>
| |
| − | <a href="images/portfolio/5_jj.jpg" rel="prettyPhoto[pp_gal]">
| |
| − | <span class="item-on-hover"><span class="hover-image"></span></span>
| |
| − | <img src="images/portfolio/5_jj.jpg" alt=" " />
| |
| − | </a>
| |
| − | <a href="images/portfolio/3_jj.jpg" rel="prettyPhoto[pp_gal]">
| |
| − | <span class="item-on-hover"><span class="hover-image"></span></span>
| |
| − | <img src="images/portfolio/3_jj.jpg" alt=" " />
| |
| − | </a>
| |
| − | <a title="JJ Royal" href="images/portfolio/6_jj.jpg" rel="prettyPhoto[pp_gal]">
| |
| − | <span class="item-on-hover"><span class="hover-image"></span></span>
| |
| − | <img alt=" " src="images/portfolio/6_jj.jpg" />
| |
| − | </a>
| |
| − | </div>
| |
| − |
| |
| | </div><!--end portfolio content--> | | </div><!--end portfolio content--> |
| | <div class="clear"></div> | | <div class="clear"></div> |