Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

Line 42: Line 42:
 
                 <td>VR</td>
 
                 <td>VR</td>
 
                 <td>ATTACCGCCTTTGAGTGAGC</td>
 
                 <td>ATTACCGCCTTTGAGTGAGC</td>
 +
            </tr>
 +
<tr class="primers-subheading">
 +
                <td colspan="2">Date rape drugs</td>
 
             </tr>
 
             </tr>
 +
 +
          <tr id="BlcR.fwd">
 +
                <td>BlcR_fwd</td>
 +
                <td>ATGGGCCAAAGGGGCCAAGT</td>
 +
            </tr>
 +
 +
          <tr id="BlcR_rev">
 +
                <td>BlcR_rev</td>
 +
                <td>CTAGTCTTTCTGCGATCGGTACCC</td>
 +
            </tr>
 +
 +
          <tr id="pSB1C3_BlcR_fwd">
 +
                <td>pSB1C3_BlcR_fwd</td>
 +
                <td>CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG</td>
 +
            </tr>
 +
 +
          <tr id="pSB1C3_BlcR_rev">
 +
                <td>pSB1C3_BlcR_rev</td>
 +
                <td>TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAG</td>
 +
            </tr>
 +
 +
          <tr id="BlcC_rev">
 +
                <td>BlcC_rev</td>
 +
                <td>TTACGCGTAAAATTCGGGAGCCTTC</td>
 +
            </tr>
 +
 +
          <tr id="pro_Blc_fwd">
 +
                <td>pro_Blc_fwd</td>
 +
                <td>ATGCACCTTCCTTGACACACT</td>
 +
            </tr>
 +
 +
          <tr id="pro_Blc_rev">
 +
                <td>pro_Blc_rev</td>
 +
                <td>CATCGACCTCTCCCGCATTAG</td>
 +
            </tr>
 +
 +
          <tr id="name">
 +
                <td>name</td>
 +
                <td>sequence</td>
 +
            </tr>
 +
 +
          <tr id="name">
 +
                <td>name</td>
 +
                <td>sequence</td>
 +
            </tr>
 +
  
 
             <tr class="primers-subheading">
 
             <tr class="primers-subheading">

Revision as of 15:18, 6 July 2015

iGEM Bielefeld 2015


Primers

Name Sequence (5´ → 3´)
Standard primers
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAG
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
name sequence
name sequence
Arsenic biosensor
Ars_1_fwd (Cm_fwd) CGGCATCAGCACCTTGTC
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_2_rev (Cm_rev) TATACGCAAGGCGACAAG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA