Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

Line 59: Line 59:
 
           <tr id="pSB1C3_BlcR_fwd">
 
           <tr id="pSB1C3_BlcR_fwd">
 
                 <td>pSB1C3_BlcR_fwd</td>
 
                 <td>pSB1C3_BlcR_fwd</td>
                 <td>CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG</td>
+
                 <td>cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG</td>
 
             </tr>
 
             </tr>
  
 
           <tr id="pSB1C3_BlcR_rev">
 
           <tr id="pSB1C3_BlcR_rev">
 
                 <td>pSB1C3_BlcR_rev</td>
 
                 <td>pSB1C3_BlcR_rev</td>
                 <td>TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAG</td>
+
                 <td>ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC</td>
 
             </tr>
 
             </tr>
  
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           <tr id="K516030proB_rev">
 
           <tr id="K516030proB_rev">
 
                 <td>K516030proB_rev</td>
 
                 <td>K516030proB_rev</td>
                 <td>GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC</td>
+
                 <td>gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC</td>
 
             </tr>
 
             </tr>
  
 
           <tr id="K516030proB_fwd">
 
           <tr id="K516030proB_fwd">
 
                 <td>K516030proB_fwd</td>
 
                 <td>K516030proB_fwd</td>
                 <td>TGAACTCATATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT</td>
+
                 <td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td>
 
             </tr>
 
             </tr>
  

Revision as of 15:34, 6 July 2015

iGEM Bielefeld 2015


Primers

Name Sequence (5´ → 3´)
Standard primers
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT
Arsenic biosensor
Ars_1_fwd (Cm_fwd) CGGCATCAGCACCTTGTC
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_2_rev (Cm_rev) TATACGCAAGGCGACAAG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA