Difference between revisions of "Team:Harvard BioDesign/Notebook"

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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/a/a3/Aalto-Helsinki_collage_9.jpg" /></div>
 
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/a/a3/Aalto-Helsinki_collage_9.jpg" /></div>
  
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<!-- September below -->
+
<!-- June below -->
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">24th-28th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/1</h2>
−
<p class="entrytext">Giant iGEM jamboree!</p></div>
+
<p class="entrytext">First week in lab! Microbiology boot camp. How to pour plates, PCR, miniprep, gel electrophoresis.
 +
Researching project ideas.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">16th</h2>
+
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 6/8</h2>
−
<p class="entrytext">Talked about synthetic biology, iGEM, and our project to students and researchers in the University of Turku.</p></div>
+
<p class="entrytext">Settle on bacterial adhesion.
 +
Further background research.
 +
Plasmid design, primer design.
 +
Choose fusion peptides.
 +
Order gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">11th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/15</h2>
−
<p class="entrytext">Our team <a href="http://areena.yle.fi/1-2923572">featured</a> on the Finnish National Radio!</p></div>
+
<p class="entrytext">PCR backbones and fim proteins.
 +
Pour Chlor and Amp plates.
 +
Gibson assembly of operon plasmid and fimH plasmid.
 +
Pili purification assay development.
 +
NEGEM Meetup #1.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">10th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/22</h2>
−
<p class="entrytext">Our team got the official patches!</p></div>
+
<p class="entrytext">Redo failed PCRs.
 +
Redo failed Gibsons.
 +
Transform and plate operon Gibson.
 +
Pili purification and agglutination assay development.
 +
Microscopy on control strains.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">9th</h2>
+
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 6/29</h2>
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<p class="entrytext">Talked about synthetic biology, iGEM, and our project to students and researchers in Tampere University of Technology.</p></div>
+
<p class="entrytext">Miniprep operon plasmid.
 +
Sent for sequencing.
 +
Unsuccessful- redo operon PCRs.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">8th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 7/6</h2>
−
<p class="entrytext">Team hoodies arrived.</p></div>
+
<p class="entrytext">Induction.
 +
Operon Gibson.
 +
Agglutination assay development.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">7th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 7/13</h2>
−
<p class="entrytext">Our team was interviewed for radio!</p></div>
+
<p class="entrytext">Induction.
 +
Edit out illegal PstI cut site from fimH.
 +
Mutagenesis to add binding peptides.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">5th</h2>
+
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 7/20</h2>
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<p class="entrytext">Science picnic in Helsinki city centre! We held a small talk and workshops for the whole family.</p></div>
+
<p class="entrytext">Induction.
 +
Transform measurement study parts.
 +
Mutagenesis to add binding peptides.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">4th</h2>
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 7/27</h2>
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<p class="entrytext">First practice session for our Giant Jamboree presentation!</p></div>
+
<p class="entrytext">Induction.
 +
Nickel binding assay.
 +
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">1st</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/2</h2>
−
<p class="entrytext">StoraEnso agreed on sponsoring us!</p></div>
+
<p class="entrytext">Induction. Induction. More Induction.
 +
Nickel binding assay.
 +
Stainless steel binding assay.
 +
Cancer binding assay.
 +
Made biobricks, sent for sequencing.</p></div>
  
  
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<!-- August below -->
 
<!-- August below -->
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">26th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week 8/9</h2>
−
<p class="entrytext">LAL -  the Finnish Union of Experts in Science agreed to sponsor us!</p></div>
+
<p class="entrytext">Induction. Induction. More Induction.
 +
Nickel binding assay.
 +
Stainless steel binding assay.
 +
Cancer binding assay.
 +
Retried failed biobricks.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">26th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/16</h2>
−
<p class="entrytext">The Faculty of Agriculture and Forestry joined our sponsors!</p></div>
+
<p class="entrytext">Break from lab.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">21st</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/23</h2>
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<p class="entrytext">Sent our GFP brick to Slovenia for validation</p></div>
+
<p class="entrytext">Break from lab.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">20th</h2>
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/30</h2>
−
<p class="entrytext">First sequencing results!</p></div>
+
<p class="entrytext">Wiki and presentation planning.
 +
</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">19th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 9/6</h2>
−
<p class="entrytext">Ordered the team hoodies.</p></div>
+
<p class="entrytext">Retried failed biobricks.
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">13th</h2>
+
Obtained results for Rhamnose-GFP inductions.
−
<p class="entrytext">Two more BioBricks-to-be sent for sequencing to the Institute of Molecular Medicine Finland!</p></div>
+
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">11th</h2>
+
Made presentation.
−
<p class="entrytext">Met with Tarja Knuuttila's group from the University and discussed our human practices project.</p></div>
+
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">7th</h2>
+
NEGEM Meetup #2!</p></div>
−
<p class="entrytext">Ordered sequencing for our first construct from Institute of Molecular Medicine Finland.</p></div>
+
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">6th</h2>
+
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 9/13</h2>
−
<p class="entrytext">Talked about iGEM to Finnish Biology Olympiad candidates.</p></div>
+
<p class="entrytext">Worked on parts pages.
 +
Worked on wiki.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">5th</h2>
+
<div class="entry"><img class="entrylogo" ></img><h2 class="day">9/24 - 9/28</h2>
−
<p class="entrytext">Attempted Gibson Assembly method first time for the gene parts. The results are very promising!</p></div>
+
<p class="entrytext">iGEM Giant Jamboree!!!</p></div>
  
  
 
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/5/59/Aalto-Helsinki_collage_7.jpg" /></div>
 
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/5/59/Aalto-Helsinki_collage_7.jpg" /></div>
−
 
−
<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/e/e5/Aalto-Helsinki_timeline_AUG.png"></img></div>
 
−
<!-- August above -->
 
−
 
−
 
−
<!-- July below -->
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">29th</h2>
 
−
<p class="entrytext">Micelle model ready!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">24-26th</h2>
 
−
<p class="entrytext">Nordic iGEM Conference in Uppsala organized by <a href="https://2015.igem.org/Team:Uppsala">iGEM Uppsala</a>! and <a href="https://2015.igem.org/Team:Stockholm">iGEM Stockholm</a>!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">23th</h2>
 
−
<p class="entrytext">Rehearsing the presentation for Uppsala.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">23th</h2>
 
−
<p class="entrytext"><a href="https://www.helsinki.fi/fi/uutiset/opiskelijat-kehittavat-biopolttoainetta-kansainvalisessa-kilpailussa">An article</a> published about us on the University of Helsinki's website</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">22nd</h2>
 
−
<p class="entrytext">Ordered third plasmid pCDFDuet-1 from Merck Life Science. The plasmid is needed to combine cellulase and propane pathways into the same bacteria.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">22nd</h2>
 
−
<p class="entrytext">Stora Enso became a sponsor!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">20th</h2>
 
−
<p class="entrytext">AYY joined our partners!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">17th</h2>
 
−
<p class="entrytext">Modeling team was given the challenge of modeling micelle formation.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">15th</h2>
 
−
<p class="entrytext">Preliminary results of bottlenecks in our pathway!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">14th</h2>
 
−
<p class="entrytext">Our first gBlocks have arrived from <a href="http://www.idtdna.com">IDT</a>!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">13th</h2>
 
−
<p class="entrytext">Our labteam figured our why our practice project didn't produce the protein we wanted - the promoter was inserted wrong.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">10th</h2>
 
−
<p class="entrytext">Met with Tarja Knuuttila and her students to discuss our human practices aspects.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">9th</h2>
 
−
<p class="entrytext">Skype meeting with <a href="https://2015.igem.org/Team:Stockholm">iGEM Stockholm</a>. Discussed our collaboration and the upcoming Nordic iGEM Conference!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">8th</h2>
 
−
<p class="entrytext">Skype meeting about collaboration with <a href="https://2015.igem.org/Team:Slovenia_HS">Slovenia HS</a>.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">7th</h2>
 
−
<p class="entrytext">Started to build the model of the cellulose pathway. Had also discussion about modeling with <a href="https://2015.igem.org/Team:Dundee">iGEM Dundee</a>.</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">6th</h2>
 
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<p class="entrytext">Modeling of propane pathway went a huge leap forward when many of the much-needed constants were finally found!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">2nd</h2>
 
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<p class="entrytext">Our first PCR reaction towards Gibson Assembly has been successful!</p></div>
 
−
 
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">1st</h2>
 
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<p class="entrytext">Modeling team had a meeting with Elena Czeizler from Aalto University's department of computer science. We also had a Skype meeting with <a href="https://2015.igem.org/Team:Edinburgh">Edinburgh team</a>.</p></div>
 
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−
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/f/f6/Aalto-Helsinki_collage_6.jpg" /></div>
 
−
 
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<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/e/e7/Aalto-Helsinki_timeline_JUL.png"></img></div>
 
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<!-- July above -->
 
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−
 
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<!-- June below -->
 
−
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">29th</h2>
 
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<p class="entrytext">Mascot is ready!</p></div>
 
−
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">27th</h2>
 
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<p class="entrytext">Ratekoulutus joined our group of sponsors!</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">26th</h2>
 
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<p class="entrytext">Ordered needed sequences to begin with Gibson Assembly</p></div>
 
−
 
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">26th</h2>
 
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<p class="entrytext">Created constructs for Interlab measurement study</p></div>
 
−
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">25th</h2>
 
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<p class="entrytext">Discussion with Anja Martinez from Thermo Fisher Scientific. She was helping us with some sequences which were too complex to be traditionally synthesized.</p></div>
 
−
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">24th</h2>
 
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<p class="entrytext">Took part to IDT webinar on gBlocks</p></div>
 
−
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">22nd</h2>
 
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<p class="entrytext">Particle model about synergy ready!</p></div>
 
−
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">17th</h2>
 
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<p class="entrytext">Registered to iGEM Interlab measurement study. The goal of the study is to obtain fluorescence data for three genetic devices with the same protocols from teams all around the world.</p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">12th</h2>
 
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<p class="entrytext">Finished planning primers for PCR and sequences for synthesis services ordered from Genescript, who is supporting us with those! The first versions of our gene constructs are also ready.</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">10th</h2>
 
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<p class="entrytext">Kemianteollisuus is going to sponsor us!</p></div>
 
−
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">9th</h2>
 
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<p class="entrytext">Started to work with our wiki.</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">9th</h2>
 
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<p class="entrytext">Lab safety training given by our instructor Heli Viskari</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">5th</h2>
 
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<p class="entrytext">Learning how to pitch at Startup Sauna Demo Day </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">4th</h2>
 
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<p class="entrytext">Learning about modelling. Merja Oja advised us about this. </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">1st</h2>
 
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<p class="entrytext">Presenting our research idea to the Department’s research group in their monthly meeting. </p></div>
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/2/2c/Aalto-Helsinki_collage_5.jpg" /></div>
 
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<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/0/0e/Aalto-Helsinki_timeline_JUN.png"></img></div>
 
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<!-- June above -->
 
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<!-- May below -->
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">28th</h2>
 
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<p class="entrytext">The modeling of our reaction pathway has started. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">27th</h2>
 
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<p class="entrytext">Meeting with yeast expert Jussi Jäntti. We discussed the possibility of using yeast as a chassis organism and other approaches to propane production with researcher Jussi Jäntti from VTT Technical Research Center of Finland.</p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">26th</h2>
 
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<p class="entrytext">Discussion with Merja Penttilä. We talked about our ideas and different ways to approach the theme of propane production with the coordinator of the synthetic biology programme Living Factories. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">25th</h2>
 
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<p class="entrytext">Webpage ready! The web page is now coded and ready to be used. Some of our members learned a bunch of new stuff of html. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">22nd</h2>
 
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<p class="entrytext">Learning to build plasmid maps. Our team member Anna showed everyone how to build plasmid maps. </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">21st</h2>
 
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<p class="entrytext">IBC Finland announced that they will sponsor us. </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">19th</h2>
 
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<p class="entrytext">First day in lab </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">18th</h2>
 
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<p class="entrytext">High school presentation at “Magpie Island”. Telling about iGEM to students from Mäkelänrinne Sports High School. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">14th</h2>
 
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<p class="entrytext">Propane production in bacteria has been decided as our research topic.</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">11th</h2>
 
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<p class="entrytext">Meeting with our Instructor Heli and a quick tour in the lab.</p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">5th</h2>
 
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<p class="entrytext">Professor meeting with Olli Ikkala. What does learning mean? How do machines and bacteria learn? How could we approach learning through synthetic biology? </p></div>
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/f/ff/Aalto-Helsinki_collage_4.jpg" /></div>
 
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<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/a/a0/Aalto-Helsinki_timeline_MAY.png"</img></div>
 
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<!-- May above  -->
 
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<!-- April below -->
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">29th</h2>
 
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<p class="entrytext">Meeting with the Aalto University School of Chemical Technology. Meeting Janne Laine, the Dean of Aalto School of Chemical Technology was at least as encouraging as the meeting with School of Science we had had a week ago. </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">23th</h2>
 
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<p class="entrytext">Student Union of the University of Helsinki (HYY) is our first sponsor! </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">21st</h2>
 
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<p class="entrytext">Meeting with the Aalto University School of Science. We met with Risto Nieminen, the Dean of Aalto School of Science. He was really excited about our project and seemed happy to help us. This made us even more excited and happy about our project! </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">16th</h2>
 
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<p class="entrytext">iGEM 2015 distribution arrived! The package including BioBricks, linearized backbones, kits, stickers and pins arrived from iGEM headquarters. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">16th</h2>
 
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<p class="entrytext">Meeting at the University of Helsinki. We met with rector Jukka Kola, the head of the University of Helsinki and Keijo Hämäläinen, vice-rector in charge of research and education. It was really exciting and encouraging to hear that the University is supporting us. </p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">1st</h2>
 
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<p class="entrytext">First skype meeting with iGEM Pasteur. Collaboration with <a href="https://2015.igem.org/Team:Pasteur_Paris">Pasteur iGEM team</a> from Paris started with a Skype meeting.</p></div>
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/0/06/Aalto-Helsinki_collage_3.jpg" /></div>
 
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<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_timeline_APR.png"</img></div>
 
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<!-- April above -->
 
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<!-- March below -->
 
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<div class="entry"><img class="entrylogobig"  ></img><h2 class="day">25th</h2>
 
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<p class="entrytext">Brainstorming. We met for this purpose for several times during the spring. Outside the meetings, we researched the various ideas we had come up with and browsed through what the teams from previous years had done and what had been done outside iGEM. </p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">22nd</h2>
 
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<p class="entrytext">First skype with the <a href="https://2015.igem.org/Team:Stockholm">Stockholm iGEM team</a>. Last year's team was also present to discuss the practical issues with starting an iGEM team from scratch. </p></div>
 
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<div class="entry"><img class="entrylogobig"  ></img><h2 class="day">19th</h2>
 
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<p class="entrytext">The first meeting of the 2015 team. Some had already met before, some of us met each other for the first time ever. A couple of team members from the 2014 team and our Advisor Markus were also present.</p></div>
 
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<div class="entry"><img class="entrylogobig" ></img><h2 class="day">14th</h2>
 
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<p class="entrytext">First anniversary of iGEM Aalto-Helsinki! On March 14th 2014 the first ever Finnish iGEM team met for the first time.</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">11th</h2>
 
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<p class="entrytext">The new team members were chosen by the 2014 team.</p></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">3-6th</h2>
 
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<p class="entrytext">Interviews held. More than 30 candidates were interviewed for the Aalto-Helsinki 2015 team by 2014 team members.</p></div>
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/7/76/Aalto-Helsinki_collage_2.jpg" /></div>
 
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<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/0/0c/Aalto-Helsinki_timeline_MAR.png"</img></div>
 
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<div class="entry"><img class="entrylogo" ></img><h2 class="day">28th</h2>
 
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<p class="entrytext">Application period for the 2015 team closed.</p></div>
 
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<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_collage_1.jpg" /></div>
 
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<div class="month" ><img  class="monthlogo" src ="https://static.igem.org/mediawiki/2015/8/81/Aalto-Helsinki_timeline_FEB.png"</img></div>
 
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Revision as of 21:35, 18 September 2015

Prologue by HTML5 UP

Our 2015 Lab Notebook!

Week of 6/1

First week in lab! Microbiology boot camp. How to pour plates, PCR, miniprep, gel electrophoresis. Researching project ideas.

Week of 6/8

Settle on bacterial adhesion. Further background research. Plasmid design, primer design. Choose fusion peptides. Order gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.

Week of 6/15

PCR backbones and fim proteins. Pour Chlor and Amp plates. Gibson assembly of operon plasmid and fimH plasmid. Pili purification assay development. NEGEM Meetup #1.

Week of 6/22

Redo failed PCRs. Redo failed Gibsons. Transform and plate operon Gibson. Pili purification and agglutination assay development. Microscopy on control strains.

Week of 6/29

Miniprep operon plasmid. Sent for sequencing. Unsuccessful- redo operon PCRs.

Week of 7/6

Induction. Operon Gibson. Agglutination assay development.

Week of 7/13

Induction. Edit out illegal PstI cut site from fimH. Mutagenesis to add binding peptides.

Week of 7/20

Induction. Transform measurement study parts. Mutagenesis to add binding peptides.

Week of 7/27

Induction. Nickel binding assay. Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.

Week of 8/2

Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Made biobricks, sent for sequencing.

Week 8/9

Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Retried failed biobricks.

Week of 8/16

Break from lab.

Week of 8/23

Break from lab.

Week of 8/30

Wiki and presentation planning.

Week of 9/6

Retried failed biobricks. Obtained results for Rhamnose-GFP inductions. Made presentation. NEGEM Meetup #2!

Week of 9/13

Worked on parts pages. Worked on wiki.

9/24 - 9/28

iGEM Giant Jamboree!!!