Difference between revisions of "Team:Technion HS Israel/Notebook"

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Revision as of 21:59, 18 September 2015

Technion 2015 HS Team's Wiki

        Top Summery Preparation July 12th July 13th July 15th July 20th July 22th July 29th August 2th August 3th August 4th August 5th August 6th August 9th August 10th August 11th August 12th August 13th August 16th August 17th August 18th /div>

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.		 we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .		 - BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.		 - BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.		 - BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

preparation
  • 1. LB medium
  • 2. Miniprep
Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5 https://2015.igem.org/File:Technion_HS_Israel_pre6 https://2015.igem.org/File:Technion_HS_Israel_pre7 https://2015.igem.org/File:Technion_HS_Israel_pre2 https://2015.igem.org/File:Technion_HS_Israel_pre3 https://2015.igem.org/File:Technion_HS_Israel_pre4

July 12th

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July 13th

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July 15th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 20th

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July 22th

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July 29th

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august 2th

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august 3th

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august 4th

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august 5th

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august 6th

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august 9th

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august 10th

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august 11th

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august 12th

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august 13th

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august 16th

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august 17th

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august 18th

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august 19th

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august 20th

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august 23th

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august 24th

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august 25th

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august 26th

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august 27th

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august 29th

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august 30th

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august 31th

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september 1th

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september 2th

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september 3th

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september 5th

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september 6th

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september 7th

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