Difference between revisions of "Team:Northeastern Boston/notebook"

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<h2>Protocols.io</h2>
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<p>Northeastern has decided to use Protocols.io to host all of its iGEM protocols. Protocols.io is an online open source wet lab repository. Researchers can upload their experimental protocol, share it others, build in functionality (like timers), and "fork" other researchers' protocols (adapting them for their particular situation).</p>
 
<p>Northeastern has decided to use Protocols.io to host all of its iGEM protocols. Protocols.io is an online open source wet lab repository. Researchers can upload their experimental protocol, share it others, build in functionality (like timers), and "fork" other researchers' protocols (adapting them for their particular situation).</p>
  
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<p>Reproducibility is the existential crisis that plagues science; when results in one lab are completely unrepeatable elsewhere, the value of science plummets. Protocols.io could be one potential solution. We enjoyed using it, forking protocols, and making our own. We encourage other teams to try it as well. Protocols don't need to be hidden in transgenerational, witchcraft-ready tomes, or lab-protected Dropboxes. Instead, these methods should be as easy to access as possible.</p>
 
<p>Reproducibility is the existential crisis that plagues science; when results in one lab are completely unrepeatable elsewhere, the value of science plummets. Protocols.io could be one potential solution. We enjoyed using it, forking protocols, and making our own. We encourage other teams to try it as well. Protocols don't need to be hidden in transgenerational, witchcraft-ready tomes, or lab-protected Dropboxes. Instead, these methods should be as easy to access as possible.</p>
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Revision as of 22:20, 18 September 2015

DNA

HiFi DNA Assembly
Plasmid Miniprep
Gel Electrophoresis
PCR with Phusion
Gel DNA Recovery
T4 Ligation
Restriction Digest

Escherichia coli

LB Agar Plates
E. coli Heat Shock

Chlamydomonas reinhardtii

TAP Agar Plates
Glass Bead Transformation

Protocols.io