Difference between revisions of "Team:UIUC Illinois/Collaborations"

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Early in the competition season, the TU_Eindhoven team reached out to us and asked for help on a collaboration. They had the excellent idea to create a cloning guide, describing each cloning method in iGEM -- such as traditional assembly, gibson assembly, and goldengate -- along with protocols, FAQs, and various tips and tricks. Each igem team wrote a section for a different cloning method, with ours being 3A Assembly. We wrote about our team’s experience with 3A assembly, shared our sources for protocols, and provided some diagrams for the guide. TU_Eindhoven got back to us several times with feedback and we revised accordingly.
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Golden Gate, Gibson, 3A, Standard Assembly – whenever you want to stitch two or more pieces of DNA together, you have a lot of options! But choosing which option to use for any particular task can be dificult, and that’s just the beginning; while each method has its advantages, they also all come with downsides or potential problems that must be troubleshooted. Thankfully for future iGEM teams, this task just got a little easier with TU_Eindhoven’s Cloning Guide, which was our collaboration project for this season.
 
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We wish we could take credit for this excellent idea, but it was the TU_Eindhoven iGEM team that reached out to us and asked if we could write a chapter of their guide for them. Realizing the benefit of such a guide, we happily agreed!
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Our team was assigned the 3A Assembly method. To complete the guide, we assembled our protocols, wrote an FAQ section, documented our personal experience with the assembly method, researched its history, and weighed the pros and cons of 3A. We also included a couple of diagrams and sources for further reading. To validate our protocol, we successfully used 3A Assembly to combine a yellow chromoprotein from the distribution kit with a promoter and a new backbone.
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We checked in several times with TU_Eindhoven throughout the competition season for feedback on the guide’s progress. After incorporating the suggestions, we sent back our final version so that it could be incorporated into the complete guide. Keep an eye out for it to check out our contribution, as well as the sections on the rest of the cloning methods, which were written by other 2015 iGEM teams. It just might make your next cloning protocol go that much smoother!
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Revision as of 22:26, 18 September 2015

Collaborations

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Golden Gate, Gibson, 3A, Standard Assembly – whenever you want to stitch two or more pieces of DNA together, you have a lot of options! But choosing which option to use for any particular task can be dificult, and that’s just the beginning; while each method has its advantages, they also all come with downsides or potential problems that must be troubleshooted. Thankfully for future iGEM teams, this task just got a little easier with TU_Eindhoven’s Cloning Guide, which was our collaboration project for this season.

We wish we could take credit for this excellent idea, but it was the TU_Eindhoven iGEM team that reached out to us and asked if we could write a chapter of their guide for them. Realizing the benefit of such a guide, we happily agreed!

Our team was assigned the 3A Assembly method. To complete the guide, we assembled our protocols, wrote an FAQ section, documented our personal experience with the assembly method, researched its history, and weighed the pros and cons of 3A. We also included a couple of diagrams and sources for further reading. To validate our protocol, we successfully used 3A Assembly to combine a yellow chromoprotein from the distribution kit with a promoter and a new backbone.

We checked in several times with TU_Eindhoven throughout the competition season for feedback on the guide’s progress. After incorporating the suggestions, we sent back our final version so that it could be incorporated into the complete guide. Keep an eye out for it to check out our contribution, as well as the sections on the rest of the cloning methods, which were written by other 2015 iGEM teams. It just might make your next cloning protocol go that much smoother!