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>Natural halophilic protein linker 1 | >Natural halophilic protein linker 1 | ||
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Revision as of 22:49, 18 September 2015
Parts
>Natural halophilic protein linker 1
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
>Natural halophilic protein linker 2
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
>Engineered rigid halophilic protein linker
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
>Engineered flexible halophilic protein linker
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG
GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG
GCGGCCGCGGATGCCCGGATCAGCCTCGCGAAGATGGCGGCGAATGAAACGGGCATGGGC
GTGATCGAGGACAAGGTCATCAAGAATCACTTCGCCTCGGAGTACATTTACAACAAGTAC
AAGGATGAGAAAACCTGTGGCATTCTGGACGAGGATCCCACGTTCGGCACGATCACCATC
GCGGAGCCGGTCGGTATCATTTGCGGGATCGTCCCGACGACGAACCCCACCTCCACGGCG
ATCTTTAAGGCCCTGATTTCGCTCAAGACCCGCAACGGCATCATCTTCTCGCCGCACCCC
CGAGCCAAGGAGTCCACCACGACGGCGGCCCGGCTCGTCCTGAATGCCGCGATCGAAGCG
GGTGCGCCCAAGGATATTATCGGGTGGATCGATGAGCCGTCGGTGGCCCTGTCGAACCAG
CTCATGACGCATGATAAGATTAACCTCATTCTCGCCACCGGCGGTCCCGGTATGGTGAAG
GCCGCCTACTCGTCGGGGAAGCCGGCCATTGGGGTGGGTGCCGGGAACACCCCCATCGTC
ATTGACGAAACCGCGGACATCAAGCGCGCGGTGTCGTCCATCCTCATGTCCAAGACCTTT
GACAACGGGGTGGTGTGCGCGTCGGAGCAAGCCGTGGTCATTGTCGACGAAATTTACGAT
ACGGTCAAGGAGCGGTTTGCCACGCACGGGGGCTACATTCTCAGCAAAGACGAGACCGCC
GCCATGCAAAATGTCATTCTGAAGAATGGCGGGCTCAACGCGGATATCGTCGGTCAGAGC
GCCGTGACCATTGCGGCGATGGCCGACATTGAGGTCCCCTCGTGGACGAAGGTCCTGATT
GGTGAGGTCGAGGATGTCTCGGAGGCGGAGGCCTTTGCGCATGAGAAGCTCAGCCCGCTG
CTGGGCATGTACCGCGTGAAGGACTTTGAAGCCGCCATGGACAAAGCCGAAGCCCTGGTC
ACCCTCGGGGGTATCGGTCACACGAGCGGTCTGTACACGAACCAAGACACGCAGACGGAA
CGCGTGATGGCGTTTGGCTTCCGCATGAAGACGGCGCGCATCCTGATCAACACGCCGGCG
TCCCAAGGTGGTATTGGCGACCTGTATAACTTTAAGCTGGCCCCCTCGCTCACGCTGGGC
TGCGGCAGCTGGGGTGGGAATTCGATTAGCGAGAACGTCGGCCCGTCGCACCTCATCAAT
AAGAAAATGGTCGCGAAACGCGCGGAGAACATGCTCTGGCACAAGCTCCCGTCGTCGATC
TACTTCCGACGGGGGTCGCTCCCGATTGCCCTCGAAGAACTCAGCGATAAAAAGCGAGCG
CTGATCGTCACGGATAAGTACCTCTTTAATAACGGCTATTGCGATGAGACCATCAAAATT
CTCAAGTCGCAGGGGCTCGAAACGGAGATCTTCTACGAAGTGGAAGCGGACCCGACGCTC
GCGATTGTCACGCAGGGCGCGAAGATGGCCCAATCGTTCCAGCCGGATGTGATCATTGCC
CTGGGTGGGGGCAGCCCGATGGATGCCGCCAAGATTATTTGGGTCATGTACGAGCACCCG
GACGTCGATTTTGCGGACCTCGCCCTCCGGTTTATGGACATTCGCAAGCGCATCTACAAG
TTCCCGAAACTGGGGGTCAAAGCGCAGATGGTGGCCATCCCGACCACGTCGGGCACCGGC
AGCGAGGTGACGCCGTTCGCGGTCGTCACCGATGAAAAGACGGGTATGAAGTACCCCATT
GCCGATTATCAACTGACGCCCAACATGGCGATCGTGGATCCGAACCTGGTGATGGATATG
CCGAAGTCGCTGACCGCCTTCGGGGGGATTGACGCGGTGACCCACGCGCTCGAGGCCTAC
GTCTCGGTCATGGCCAACGAATACTCGGACGGCCAGGCGCTCCAAGCGCTGGATCTCCTC
TTCAAGCACCTCCCGGACAGCTACAACCACGGTGCCGCGGCGCCCATCGCCCGGGAAAAG
GTCCACAATGGCGCGACGATTGCGGGGATCGCGTTCGCCAACGCCTTCCTCGGGATTTGT
CACAGCATGGCCCATAAGCTGGGGGCCGAATTTCACCTGGCCCACGGGCTCGCCAATGCC
CTGCTCATTAACAACGTCATCCGGTTCAACGCGACGGACATGCCGACGAAGCAGGCGGCG
TTTTCGCAGTACGATCGACCCAAGGCGCTGTGCCGCTACGCGAAAATCGCCGAATATCTG
AACCTGGGCGGTACGACGGATGAGGAGAAGGTGGAGGCCCTCATTGAAGAAGTCGACCAG
CTGAAAAAAACCATTGGTATTCCGGCGAGCATTCGAGAGGCGGGTGTCAACGAGGCCGAC
TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC
AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
ACGGCCTATTCGGACAAGTGA
>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC
CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA
AACATGGAGCAGGTCTACTGCTGCAACGAACTGAATTGCGGTTTTTCCGCCGAGGGCTAC
GCGCGAGCGAAAGGTGCCGCGGCGGCGGTCGTGACGTACTCGGTCGGGGCCCTGTCGGCC
TTCGACGCGATCGGAGGAGCGTATGCGGAGAACCTCCCCGTCATCCTGATCTCGGGTGCG
CCGAATAACAACGATCATGCCGCCGGTCACGTCCTCCACCACGCCCTGGGCAAGACGGAT
TACCACTACCAGCTCGAAATGGCGAAGAACATTACCGCCGCCGCGGAAGCCATCTACACG
CCCGAAGAGGCGCCGGCCAAGATTGATCATGTCATTAAGACGGCCCTGCGAGAGAAAAAG
CCGGTCTACCTCGAGATCGCCTGTAACATTGCCTCGATGCCGTGTGCCGCCCCCGGTCCC
GCGTCGGCGCTCTTCAATGATGAAGCCTCGGACGAAGCGTCGCTCAATGCGGCCGTCGAG
GAGACGCTGAAATTCATCGCCAACCGCGACAAGGTGGCGGTCCTCGTCGGCTCGAAGCTG
CGAGCCGCGGGTGCCGAGGAAGCCGCCGTCAAGTTCGCGGATGCGCTGGGGGGAGCGGTC
GCCACGATGGCGGCCGCGAAAAGCTTCTTCCCGGAAGAGAACCCGCACTATATCGGTACG
TCGTGGGGTGAGGTCTCCTATCCGGGTGTGGAGAAGACGATGAAGGAGGCGGACGCGGTC
ATCGCGCGCTCGCCCCGGTGTTCAACGACTACAGCACCACGGGCTGGACGGACATCCCGG
ACCCGAAAAAACTCGTGCTCGCGGAGCCGCGATCCGTCGTCGTGAATGGCATTCGCTTCC
CGTCGGTCCACCTCAAGGACTACCTCACGCGCCTGGCCCAAAAGGTCTCGAAGAAAACCG
GAGCCCTGGACTTCTTCAAGTCGCTGAATGCCGGTGAACTCAAGAAAGCGGCGCCCGCGG
ATCCCTCCGCCCCGCTCGTGAACGCGGAAATCGCCCGCCAGGTCGAAGCGCTCCTGACGC
CGAACACGACGGTGATTGCCGAGACGGGCGACTCGTGGTTCAATGCCCAACGCATGAAGC
TCCCGAACGGAGCGCGGGTCGAATACGAGATGCAGTGGGGCCACATCGGCTGGTCGGTGC
CGGCCGCCTTCGGCTATGCGGTGGGAGCCCCGGAACGGCGAAATATTCTCATGGTCGGCG
ACGGCTCGTTTCAGCTCACGGCGCAGGAGGTCGCCCAGATGGTCCGACTGAAACTCCCGG
TCATTATCTTCCTCATCAACAACTACGGCTACACGATCGAGGTCATGATCCATGATGGGC
CCTACAACAACATCAAAAACTGGGACTACGCGGGCCTGATGGAAGTCTTCAACGGCAATG
GCGGATACGACAGCGGTGCGGGCAAGGGTCTCAAAGCGAAAACGGGTGGCGAACTGGCCG
AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG
GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
GCCGCAAACCGGTCAACAAGCTCCTCTGA
>Codon Optimized Haloarcula Hispanica beta-glucosidase
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG
ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA
CCGGACGTCGACAGTCTCATTGGCGACCTCACCCTCGAGCAGAAAGCGGCCCAGATGACG
CAGGTCGCGATCTCGTCGTTCGAAGCCGAACCGGAAGAGTCGAATGTGCCGGATTCGTTT
GGGGTCGACACGGTCGGCGAGTACTTCAGCGAGCTGGGCATCGGGTCGATCCTGTCGGGT
GGCGCGGAACCGCCGTCGTTTGACGCGAACGCGGTCGTCCAGGGGATCAACGCCCTGCAG
CAATACAACCTCGACAACGCGGACCACGACATCCCGTTCCTCTACGGGGTGGATGCGACG
CACGGCAACGGCCTCCTGGAAGGGGCCACCGTCTTCCCGCAACGCCTCAACATGGGCGTC
ACGCGAGATCTGTCGCTCATCGCCGAAGCGGAACGCCACACGTCGGACGCCACGGCGAGC
ATGGGTGCCCATTGGACCTTTGCGCCGACGACGGACCTCCAACGCGACCCGCGCTGGGGC
CGATTTTTCGAAGGCATCTCGGAAGACCCGAAGCTGGAGGCGGATGTCAGCCGAGCCCGA
GCGCGAGCCCTCGAAGACGACGATCGCCTCACGGCCTGCGTGAAGCACTTCGCGGCGTAC
TCGGTGCCGAACAATGGCAATGATCGAGCGCCCGCCAGCACGAGCCTCCGAGACCTCCGC
ACGAACATCCTCCCGCCGTACCGAGAGGCGCTGGAATCGGAGCCCGGCACGGTCATGGTC
AACTCGGGTAGCATCAACGGCGTGCCCGCCCACGCCTCCCACTGGCTCCTCACGACGCTC
CTCCGCGACACGTACGGCTACGAGGGGATGGTCGTGTCGGATTGGGACGATCTCAATCGC
ATGATCACGAATCACGACTATGCCCCCGATTTCGAGACGGCGACGGAGATGGCGATCAAT
GCGGGCGTCGATATGTATATGATCGGTAACGGTGGGGATGCCCCGGGTCCCGTCCAATTC
ATCGACACCGTGGTGTCGCTGGTCGAGGACGGTGCGATCCCGATGGAGCGAATCGACGAG
GCGGTCCGCCGCATTCTCGAGCTCAAGGCCGACCTGGGCCTCTTTGAACAACCGACGGTC
GACGAATCGCGAATTGAGACGGTCCTGGGTGGTGCGCAGGAGACCGCCGAGACGATGGCG
AAAGAGTCGATGGTGCTCCTGAAGAACACCGACGATACGCTCCCGCTCTCGGGCGATGAG
TCCGTCCTCCTGACGGGTCCGGGTGTGGACTCGAATGGCAATAATACGCGAGCCCTCATG
CAGCATGGTGGTTGGACGCTCGGCTGGCAAGGCGCCAGCGCCGGTGGCCCGTTTCCGCGC
CAGAACCTGCTGGAGGCCGAACTCCGAGCGCGAGTCGGCTCCCTCACGCACGTCCCGACG
AGCTACGAGAATACCACCTGGTGGGCGGGTGAAGGCGACGGCGAGAACCAGCAGTCGGAT
GAAAATGGTAATTTCGACTTCACGGCGGAGCAGCGCTCGCGGGTCGAATCCGCCGGTCCC
GAATCGGATGTGGTGGTCGTGGTCCTGGGGGAAGGCACGCACAACGAGGGCTTCGGCGAC
CGAGATGAACTCGTGCTCGACGAGTCGCAGCAGGCGCTCCTCGATACCGTGGTGGAATCG
ACGGATGACAGCACGCCGATCATCGGGGTCATGCTGGCCGGCTCGCCCCGAGGCTCCCCC
GAGACGTTTAGCCAGCTCGATGCCCTCCTCTTTGCGGGTCAACCGGGTAGCGACGGGGGT
GTCGCGATTGCGGAGACGCTGGTCGGCGAGTACAACCCCTCGGGTAAGCTCCCGTTCTCG
TGGCCGGAGAATGTCGGCACGACCCCGGTCCAGTACACGCGCTACGACCCGACGTCCACG
GGTGGTACGGACAACACGGCGATCTACGAATATGGGCACGGCCTGTCGTACACGGATTTC
GAGTACGGCTCGGTCAGCGTGACGCAGCCGACCGTCGGCAGCCCGGCCAACCAATCCGAG
GTCACCGTGTCGGTGGAGGTGACGAACACGGGTCGCATGGCCGGTGAGCACATTGTCGAG
GTCTTCAACACGCAGTCGTATGGGACGGTCCTCCAACCGCGACGACGACTCCTCGGTTAC
GAACGCGTCTCGCTGGGTCCCGGGGAACTGACGACCGTGGATGTCACGGTGGATCTCACG
GCCCTGGAAGTGGTGCCCGGTGACGTGCTGGGCCTCGGTCCGAAGGTCGTCGAAGCGGGG
GAATACGAGCTGACGGTGGGCCAAGACGGCCCGACGGCCACCCTCACCGTCCAGAATACG
GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC
GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG
GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG