Difference between revisions of "Team:Northeastern Boston/BasicParts"

 
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<img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%">
 
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<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p>
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<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygromycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p>
  
 
<p>This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</a>]</p>
 
<p>This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</a>]</p>

Latest revision as of 23:01, 18 September 2015

BASIC & COMPOSITE PARTS

BBa_K1857001 Hygromycin B Resistance Cassette

Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygromycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.

This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]

BBa_K1857000 mVenus Fluorescent Protein

This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.