Difference between revisions of "Team:Colegio EmelinaU/Notebook"

Line 395: Line 395:
 
                         <div class="intro">
 
                         <div class="intro">
 
<h1>Journal of Laboratory</h1>
 
<h1>Journal of Laboratory</h1>
−
<hr>
 
  
−
<strong>May 30th, 2015</strong>
+
<h2>May 30th, 2015</h2>
−
<br>
+
−
<p>- Genetic transformation of red coloration [B1010]</p>
+
  
−
<p>- Plasmid removing: [K909008]= tetR-DBD-UVR8</p>
+
Genetic transformation of red coloration [B0010]
−
<p>[B1010]= Terminator</p>
+
Plasmid removing: [K909008]= tetR-DBD-UVR8
−
<p>[R0040]= Promoter</p>
+
[B0010]= Terminator
−
+
[R0040]= Promoter
−
<hr>
+
−
<strong>June 13th, 2015</strong>
+
−
<br>
+
−
<p>- Genetic transformation of [ B1010 ] * failed . We make a new attempt</p>
+
−
<p>- Electrophoresis : [ K909008 ]</p>
+
−
<p>[ B1010 ]</p>
+
−
<p>[ R0040 ]</p>
+
−
<hr>
+
−
<strong>June 20th, 2015</strong>
+
−
<br>
+
−
<p>- Expanded coloring [ B1010 ]</p>
+
−
<p>- Make plasmid removing [ B1010 ]</p>
+
−
<p>- Perform digests [ B1010 ] = ( Terminator ) Digestion</p>
+
−
<p>[ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion</p>
+
−
<p>- electrophoresis : [ B1010 ] 16746p</p>
+
−
<p>[ K909008 ] 21506p</p>
+
−
<hr>
+
−
<strong>June 27th, 2015</strong>
+
−
<br>
+
−
<p>- Purification : [ B0010 ]</p>
+
−
<p>- [ K909008 ]</p>
+
−
<p>- Plasmid removing : [ E1010 ]</p>
+
−
<p>- Electrophoresis : [ B0010 ]</p>
+
−
<p>[ K909008 ]</p>
+
−
<p>[ E1010 ]</p>
+
−
<hr>
+
−
<strong>July 04th, 2015</strong>
+
−
<br>
+
−
<p>- Plasmid removing: [J23119]</p>
+
−
<p>[B0010]</p>
+
−
<p>[K909008]</p>
+
−
<p>[R0040]</p>
+
−
<p>[E1010]</p>
+
−
<p>-Electrophoresis: [B0010]</p>
+
−
<p>[R0040]</p>
+
−
<p>[K909008]</p>
+
−
<p>[J23119]</p>
+
−
<p>- Digestion: [B0010]</p>
+
−
<p>[K909008]</p>
+
−
<hr>
+
−
<strong>July 13th, 2015</strong>
+
−
<br>
+
−
<p>- Electrophoresis</p>
+
−
<p>- Plasmid Removing</p>
+
−
<p>- Terminator digestion and teR-DBD-UVR8</p>
+
−
<br>
+
−
<hr>
+
−
<strong>July 14th, 2015</strong>
+
−
<br>
+
−
<p>- Bands Purification: <p>[K909008]</p>
+
−
<p>[B0010]</p>
+
−
<p>Join [K909008]</p>
+
−
<p>[B0010]</p>
+
−
<p>- Electroophoresis: July 04th = [K909008]; [J23119]; [B0010]; [R0040]
+
−
[K909008]; [R0040]; [B0010]; [R0040]</p>
+
−
<p>- Digestion </p>
+
−
<br>
+
−
<hr>
+
  
−
<strong>July 24th, 2015</strong>
+
<h2>June 13th, 2015</h2>
−
<br>
+
−
<p> - Digestion= <p>[K909008]= Xbal-PSTI</p>
+
−
<p>[J23119]= SPEI-PSTI</p>
+
−
<p>[B0010]= EcoRI-Xbal</p>
+
−
<p>[R0040]= SPEI-PSTI</p>
+
−
<p>[E1010]= Xbal-PSTI</p>
+
−
<br>
+
−
<hr>
+
−
<strong>August 1st, 2015</strong>
+
−
<br>
+
−
<p>- Digestion: A[B0010]= E X I</p>
+
  
−
<p> A [R0040]= S P</p>
+
* Genetic transformation of [ B0010 ] * failed . We make a new attempt
−
<p>A [K909008]= X P II</p>
+
• Electrophoresis : [ K909008 ]
−
<p> A [J23119]= SPEI PSTI</p>
+
[ B0010 ]
−
<p> R [R0040]= S P I</p>
+
[ R0040 ]
−
<p>N [R0040]= S P I</p>
+
−
<p>N [K909008]= X P II</p>
+
−
<p> N [B0010]= E X I</p>
+
−
<p>- Genetic Transformation: [J23119]= constitutive promoter</p>
+
−
<hr>
+
−
<p>[K1084103]= RBS</p>
+
−
<p>[K909008]= tetR-DBD-UVR8</p>
+
−
<p>[B0010]= Terminator</p>
+
−
<p>[R0040]= Promoter</p>
+
−
<p>[K592009]= purple color</p>
+
−
<p>[K592011]= blue color</p>
+
−
<br>
+
−
<hr>
+
−
<strong>August 22nd, 2015</strong>
+
−
<br>
+
−
<p>- Digestion: [K1084103]</p>
+
−
<p>- Purification: [K909008]</p>
+
−
<p>- Genetic transformation: [K592009]</p>
+
−
<br>
+
−
<hr>
+
−
<strong>August 29th, 2015</strong>
+
−
<br>
+
−
<p>-Plasmid removing: [K592009]</p>
+
−
<p>-Purification: [K909008] Black</p>
+
−
<p>[K909008] blue<p>
+
−
<br>
+
  
 +
<h2>June 20th, 2015</h2>
  
 +
• Expanded coloring [ B0010 ]
 +
• Make plasmid removing [ B0010 ]
 +
• Perform digests [ B0010 ] = ( Terminator ) Digestion
 +
[ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion
 +
• electrophoresis : [ B0010 ] 16746p
 +
[ K909008 ] 21506p
 +
 +
<h2>June 27th, 2015</h2>
 +
 +
Purification : [ B0010 ]
 +
[ K909008 ]
 +
• Plasmid removing : [ E1010 ]
 +
• Electrophoresis : [ B0010 ]
 +
[ K909008 ]
 +
[ E1010 ]
 +
 +
<h2>July 04th, 2015</h2>
 +
 +
 Plasmid removing: [J23119]
 +
[B0010]
 +
[K909008]
 +
[R0040]
 +
[E1010]
 +
 Electrophoresis: [B0010]
 +
[R0040]
 +
[K909008]
 +
[J23119]
 +
 Digestion: [B0010]
 +
[K909008]
 +
<h2>July 13th, 2015</h2>
 +
 Electrophoresis
 +
 Plasmid Removing
 +
 Terminator digestion and teR-DBD-UVR8
 +
 +
<h2>July 14th, 2015</h2>
 +
 +
 Bands Purification: [K909008]
 +
[B0010]
 +
 Join [K909008]
 +
[B0010]
 +
 Electroophoresis:July04th= [K909008];[J23119];[B0010];[R0040]
 +
[K909008]; [R0040]; [B0010]; [R0040]
 +
 Digestion
 +
 +
<h2>July 24th, 2015</h2>
 +
 +
 Digestion= [K909008]= Xbal-PSTI
 +
[J23119]= SPEI-PSTI
 +
[B0010]= EcoRI-Xbal
 +
[R0040]= SPEI-PSTI
 +
[E1010]= Xbal-PSTI
 +
 +
<h2>August 1st, 2015</h2>
 +
 +
 Digestion: [B0010]= E X I
 +
[R0040]= S P
 +
[K909008]= X P II
 +
[J23119]= SPEI PSTI
 +
[R0040]= S P I
 +
[R0040]= S P I
 +
[K909008]= X P II N
 +
[B0010]= E X I
 +
 Genetic Transformation: [J23119]= constitutive promoter
 +
[K1084103]= RBS
 +
[K909008]= tetR-DBD-UVR8
 +
[B0010]= Terminator
 +
[R0040]= Promoter
 +
[K592009]= purple color
 +
[K592011]= blue color
 +
 +
<h2>August 22nd, 2015</h2>
 +
 +
 Digestion: [K1084103]
 +
 Purification: [K909008]
 +
 Genetic transformation: [K592009]
 +
August 29th, 2015
 +
 Plasmid removing: [K592009]
 +
 Purification: [K909008] Black
 +
[K909008] blue
 
</div>
 
</div>
  

Revision as of 23:10, 18 September 2015

Journal of Laboratory

May 30th, 2015

 Genetic transformation of red coloration [B0010]  Plasmid removing: [K909008]= tetR-DBD-UVR8 [B0010]= Terminator [R0040]= Promoter

June 13th, 2015

* Genetic transformation of [ B0010 ] * failed . We make a new attempt • Electrophoresis : [ K909008 ] [ B0010 ] [ R0040 ]

June 20th, 2015

• Expanded coloring [ B0010 ] • Make plasmid removing [ B0010 ] • Perform digests [ B0010 ] = ( Terminator ) Digestion [ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion • electrophoresis : [ B0010 ] 16746p [ K909008 ] 21506p

June 27th, 2015

Purification : [ B0010 ] [ K909008 ] • Plasmid removing : [ E1010 ] • Electrophoresis : [ B0010 ] [ K909008 ] [ E1010 ]

July 04th, 2015

 Plasmid removing: [J23119] [B0010] [K909008] [R0040] [E1010]  Electrophoresis: [B0010] [R0040] [K909008] [J23119]  Digestion: [B0010] [K909008]

July 13th, 2015

 Electrophoresis  Plasmid Removing  Terminator digestion and teR-DBD-UVR8

July 14th, 2015

 Bands Purification: [K909008] [B0010]  Join [K909008] [B0010]  Electroophoresis:July04th= [K909008];[J23119];[B0010];[R0040] [K909008]; [R0040]; [B0010]; [R0040]  Digestion

July 24th, 2015

 Digestion= [K909008]= Xbal-PSTI [J23119]= SPEI-PSTI [B0010]= EcoRI-Xbal [R0040]= SPEI-PSTI [E1010]= Xbal-PSTI

August 1st, 2015

 Digestion: [B0010]= E X I [R0040]= S P [K909008]= X P II [J23119]= SPEI PSTI [R0040]= S P I [R0040]= S P I [K909008]= X P II N [B0010]= E X I  Genetic Transformation: [J23119]= constitutive promoter [K1084103]= RBS [K909008]= tetR-DBD-UVR8 [B0010]= Terminator [R0040]= Promoter [K592009]= purple color [K592011]= blue color

August 22nd, 2015

 Digestion: [K1084103]  Purification: [K909008]  Genetic transformation: [K592009] August 29th, 2015  Plasmid removing: [K592009]  Purification: [K909008] Black [K909008] blue