Difference between revisions of "Team:SPSingapore/Notebook-Week-13"

 
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Latest revision as of 23:19, 18 September 2015


Research Notebook

Week 13 (16/8 - 22/8)

▪ Aug 18

Anaerobic Promoter    ☀ Yi Han ☀

PCR for the clones of pNirB-gfp from B21(1-4) and dH5alpha (1-4)with pNirB-gfp-F/R primers
5ul plasmid, 5ul H2O in each tube
PCR Mastermix
dNTP 8ul
MgCl2 20ul
Taq Polymerase 2ul
Buffer 40ul
F/R primers 0.8ul each
H2O 40ul


▪ Aug 19

Anaerobic Promoter    ☀ Chi Yan ☀

Colony PCR for pEFYP plated on (17/8)
PCR Master mix For pEYFP
H2O 13.25ul
Buffer 5ul
dNTP 5ul
F/R primer 5ul
2.5mM MgCl2 12.5
GoTaq 11.25ul


PCR for pNirBgfp as with (18/8) with pNirB-gfp primers, Biobricks prefix and suffix primers
(Melting temperature increased to 53 degrees)
3ul of plasmid in each tube
PCR Master mix for pNirB-gfp primers and BB prefix/suffix primers
H2O 101.25ul
Buffer 45ul
dNTP 9ul
Forward Primer 9ul
Reverse Primer 9ul
2.5mM MgCl2 22.5ul
GoTaq polymerase 2.25ul