Difference between revisions of "Team:London Biohackspace/experiments/yeast-culturing"

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                         <h4>Introduction</h4>
 
                         <h4>Introduction</h4>
                             <p>Yeast sediment from unfiltered craft beer was grown on YEPD agar plates to provide the source strains for the SYNBIO Brewery DIY-BrewKit. We also cultured a number of yeast strains purchased from home brew equipment suppliers.</p>
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                             <p>Over time, beer makers have evolved many different strains of yeast to ferment sugars into a wide variety of beers (and wines!) with a multitude of different properties.  We decided to use these some of these strains as the starting point for our own syn-bio beer making adventures. Yeast strains derived from unfiltered bottled beer as well as strain purchased from home brew equipment suppliers were grown on YEPD agar plates for use in all subsequent experiments.</p>
 
                         <h4>Materials and methods</h4>
 
                         <h4>Materials and methods</h4>
                        <h4>Results</h4>
 
 
<img src="https://static.igem.org/mediawiki/2015/1/13/LONBIO-extracting-yeast-from-beer-bottles.png" style="border:solid 1px black;margin-top:5px;"/>
 
<img src="https://static.igem.org/mediawiki/2015/1/13/LONBIO-extracting-yeast-from-beer-bottles.png" style="border:solid 1px black;margin-top:5px;"/>
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                        <p>Yeast sediment from the bottom of an unfiltered bottle of beer was plated onto a YEPD agar plate using a sterile innoculum loop.  Plates were left to grow at room temperature for 2-3 days until visibe colonies began to appear.</p>
 +
                        <h4>Results</h4>
 +
 
                         <h4> Discussion</h4>     
 
                         <h4> Discussion</h4>     
 
                        
 
                        

Revision as of 00:12, 19 September 2015

Experiments

CULTURING 'WILD' S. CEREVISIAE

Introduction

Over time, beer makers have evolved many different strains of yeast to ferment sugars into a wide variety of beers (and wines!) with a multitude of different properties. We decided to use these some of these strains as the starting point for our own syn-bio beer making adventures. Yeast strains derived from unfiltered bottled beer as well as strain purchased from home brew equipment suppliers were grown on YEPD agar plates for use in all subsequent experiments.

Materials and methods

Yeast sediment from the bottom of an unfiltered bottle of beer was plated onto a YEPD agar plate using a sterile innoculum loop. Plates were left to grow at room temperature for 2-3 days until visibe colonies began to appear.

Results

Discussion