Difference between revisions of "Team:SZU China/Notebook"

Line 200: Line 200:
 
}
 
}
 
}
 
}
−
        a{
 
−
            color:#ff3;
 
−
          }
 
  
 
</style>
 
</style>
Line 254: Line 251:
 
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
−
                 <p>Improved the original BioBrick TERT<a href="http://parts.igem.org/Part:BBa_K1722001">(BBa_K1722001)</a></p>
+
                 <p>Improved the original BioBrick TERT<a href="http://parts.igem.org/Part:BBa_K1722001" style="color:#ff3">(BBa_K1722001)</a></p>
 
</div>
 
</div>
 
</li>
 
</li>
Line 296: Line 293:
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
−
<p>Finished some BioBricks:shTERT+GFP Composite<a href="http://parts.igem.org/Part:BBa_K1722009">(BBa_K1722009)</a>, TERT part<a href="http://parts.igem.org/Part:BBa_K1722002">(BBa_K1722002)</a>, shTERT part<a href="http://parts.igem.org/Part:BBa_K1722001">(BBa_K1722001)</a>,hUPII part<a href="http://parts.igem.org/Part:BBa_K1722000">(BBa_K1722000)</a> and Rlu part<a href="http://parts.igem.org/Part:BBa_K1722005">(BBa_K1722005)</a>.</p>
+
<p>Finished some BioBricks:shTERT+GFP Composite<a href="http://parts.igem.org/Part:BBa_K1722009" style="color:#ff3">(BBa_K1722009)</a>, TERT part<a href="http://parts.igem.org/Part:BBa_K1722002" style="color:#ff3">(BBa_K1722002)</a>, shTERT part<a href="http://parts.igem.org/Part:BBa_K1722001" style="color:#ff3">(BBa_K1722001)</a>,hUPII part<a href="http://parts.igem.org/Part:BBa_K1722000" style="color:#ff3">(BBa_K1722000)</a> and Rlu part<a href="http://parts.igem.org/Part:BBa_K1722005" style="color:#ff3">(BBa_K1722005)</a>.</p>
 
</div>
 
</div>
 
</li>
 
</li>
Line 310: Line 307:
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
−
<p>Finished some BioBricks:SV40(en) part<a href="http://parts.igem.org/Part:BBa_K1722006">(BBa_K1722006)</a>, SV40+Rlu Composite<a href="http://parts.igem.org/Part:BBa_K1722013">(BBa_K1722013)</a>, tRNA part<a href="http://parts.igem.org/Part:BBa_K1722004">(BBa_K1722004)</a>, SV40(en)+Rlu Composite<a href="http://parts.igem.org/Part:BBa_K1722012">(BBa_K1722012)</a></p>
+
<p>Finished some BioBricks:SV40(en) part<a href="http://parts.igem.org/Part:BBa_K1722006" style="color:#ff3">(BBa_K1722006)</a>, SV40+Rlu Composite<a href="http://parts.igem.org/Part:BBa_K1722013" style="color:#ff3">(BBa_K1722013)</a>, tRNA part<a href="http://parts.igem.org/Part:BBa_K1722004" style="color:#ff3">(BBa_K1722004)</a>, SV40(en)+Rlu Composite<a href="http://parts.igem.org/Part:BBa_K1722012" style="color:#ff3">(BBa_K1722012)</a></p>
 
</div>
 
</div>
 
</li>
 
</li>
Line 333: Line 330:
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
−
<p>Finished the rest of BioBricks:ShTERT+tRNA Composite<a href="http://parts.igem.org/Part:BBa_K1722011">(BBa_K1722011)</a>,hTERT+tRNA Composite<a href="http://parts.igem.org/Part:BBa_K1722010">(BBa_K1722010）</a>，hUPll+AckRS Composite<a href="http://parts.igem.org/Part:BBa_K1722007">(BBa_K1722007)</a>
+
<p>Finished the rest of BioBricks:ShTERT+tRNA Composite<a href="http://parts.igem.org/Part:BBa_K1722011" style="color:#ff3">(BBa_K1722011)</a>,hTERT+tRNA Composite<a href="http://parts.igem.org/Part:BBa_K1722010" style="color:#ff3">(BBa_K1722010）</a>，hUPll+AckRS Composite<a href="http://parts.igem.org/Part:BBa_K1722007" style="color:#ff3">(BBa_K1722007)</a>
  
 
</p>
 
</p>
Line 349: Line 346:
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
−
<p>Finished collecting BioBricks data, and edited in the wiki.<a href="https://2015.igem.org/Team:SZU_China/Basic_parts">https://2015.igem.org/Team:SZU_China/Basic_parts</a></p>
+
<p>Finished collecting BioBricks data, and edited in the wiki.<a href="https://2015.igem.org/Team:SZU_China/Basic_parts" style="color:#ff3">https://2015.igem.org/Team:SZU_China/Basic_parts</a></p>
 
</div>
 
</div>
 
</li>
 
</li>
Line 382: Line 379:
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
 
<p>These are our note PDFs for download:<br>
 
<p>These are our note PDFs for download:<br>
−
<a href="https://static.igem.org/mediawiki/2015/c/c4/SZU_China_pdf_1.pdf">2015 SZU igem note protocol 1.0</a><br>
+
<a href="https://static.igem.org/mediawiki/2015/c/c4/SZU_China_pdf_1.pdf" style="color:#ff3">2015 SZU igem note protocol 1.0</a><br>
−
<a href="https://static.igem.org/mediawiki/2015/3/3e/SZU_China_pdf_2.pdf">2015 SZU igem note protocol 2.0</a><br>
+
<a href="https://static.igem.org/mediawiki/2015/3/3e/SZU_China_pdf_2.pdf" style="color:#ff3">2015 SZU igem note protocol 2.0</a><br>
−
<a href="https://static.igem.org/mediawiki/2015/1/10/SZU_China_NB_PDF_3.pdf">2015 SZU igem note protocol 3.0</a>
+
<a href="https://static.igem.org/mediawiki/2015/1/10/SZU_China_NB_PDF_3.pdf" style="color:#ff3">2015 SZU igem note protocol 3.0</a>
  
 
   
 
   

Revision as of 00:46, 19 September 2015



Notebook




  • To discuss the decision of team project: CANCER SNIPER BLADDER

  • Finished the project system in shuttle vector

  • In order to verify the function of our UAA orthogonal system,We constructed the whole system into three plasmids,and then we transfected the three-plasmid-system in T24 bladder cancer cells.We got a good result in Rluc(with amber mutation) detection.

  • Improved the original BioBrick TERT(BBa_K1722001)

  • In order to verify the function of hTERT and hUPII,We constructed the whole system into two plasmids,and then we transfected the two-plasmid-system in 5637 bladder cancer cells.We got a good result in Luc(with amber mutation) detection.At the same time,we transfected the two-plasmid-system in HFC and Hela to build controll groups.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Online2015SZU-iGEM Questionnaire

  • We distributed our paper questionnaiers to many places.For instence,People's Hospital of Guangdong Province,Shenzhen Hospital and Genitourinary Center of Peking University Hospital.Via our offorts,we got many important data for our project.

  • Propaganda synthetic biology and iGEM to all students at the Cultural Festival of Collage of Life Sciences Shenzhen University.

  • Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

  • Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

  • Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

  • LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

  • The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

  • Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010），hUPll+AckRS Composite(BBa_K1722007)

  • Prepared wiki,continued the system verification experiment

  • Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Basic_parts

  • we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

  • In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

  • According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.

  • These are our note PDFs for download:
    2015 SZU igem note protocol 1.0
    2015 SZU igem note protocol 2.0
    2015 SZU igem note protocol 3.0