Difference between revisions of "Team:Hong Kong-CUHK/Results"
Angellukpy25 (Talk | contribs) |
|||
Line 35: | Line 35: | ||
</div> | </div> | ||
− | <p><font size="1">Figure 1: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of Recombination Template for mamAB Operon. Lane 2: PCR product of Recombination Template for mamXY, mamGC and mms Operons. </font></p> | + | <p><font size="1.2">Figure 1: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of Recombination Template for mamAB Operon. Lane 2: PCR product of Recombination Template for mamXY, mamGC and mms Operons. </font></p> |
<br> | <br> | ||
Line 46: | Line 46: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 2: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamAB Operon (<a href="http://parts.igem.org/Part:BBa_K1648000">BBa_K1648000</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. Lane 4-6: Recombination Template for mamAB Operon with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> | + | <p><font size="1.2">Figure 2: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamAB Operon (<a href="http://parts.igem.org/Part:BBa_K1648000">BBa_K1648000</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. Lane 4-6: Recombination Template for mamAB Operon with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> |
<br> | <br> | ||
Line 56: | Line 56: | ||
</div> | </div> | ||
<br> | <br> | ||
− | <p><font size="1">Figure 3: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamXY, mamGC and mms Operons with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648003">BBa_K1648003</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> | + | <p><font size="1.2">Figure 3: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamXY, mamGC and mms Operons with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648003">BBa_K1648003</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> |
<br> | <br> | ||
Line 67: | Line 67: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 4: SDS-PAGE showing expression of Recombination Template for mamAB Operon with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>) in Azotobacter vinelandii. L: Protein ladder. Lane 1: Wild-type Azotobacter vinelandii. Lane 2: transformed Azotobacter vinelandii. Lane 2 shows 3 more bands compared to lane 1, in which the ~25 kDa band is the protein coded by chloramphenicol resistant gene in <a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>, the ~15 kDa and ~ 13kDa bands are the protein coded by Recombination Template for mamAB Operon.</font></p> | + | <p><font size="1.2">Figure 4: SDS-PAGE showing expression of Recombination Template for mamAB Operon with Promotor and Terminator (<a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>) in Azotobacter vinelandii. L: Protein ladder. Lane 1: Wild-type Azotobacter vinelandii. Lane 2: transformed Azotobacter vinelandii. Lane 2 shows 3 more bands compared to lane 1, in which the ~25 kDa band is the protein coded by chloramphenicol resistant gene in <a href="http://parts.igem.org/Part:BBa_K1648002">BBa_K1648002</a>, the ~15 kDa and ~ 13kDa bands are the protein coded by Recombination Template for mamAB Operon.</font></p> |
<br> | <br> | ||
Line 78: | Line 78: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 5: The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6. </font></p> | + | <p><font size="1.2">Figure 5: The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6. </font></p> |
<br> | <br> | ||
Line 96: | Line 96: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 6: The photo of 1% agarose gel electrophoresis showing PCR products. (A) L: DNA ladder. Lane 1: PCR products of linear Insertion Kit. (B) L: DNA ladder. Lane 2: GFP-nanobody.</font></p> | + | <p><font size="1.2">Figure 6: The photo of 1% agarose gel electrophoresis showing PCR products. (A) L: DNA ladder. Lane 1: PCR products of linear Insertion Kit. (B) L: DNA ladder. Lane 2: GFP-nanobody.</font></p> |
<br> | <br> | ||
Line 107: | Line 107: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 7: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (<a href="http://parts.igem.org/Part:BBa_K1648005">BBa_K1648005</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.</font></p> | + | <p><font size="1.2">Figure 7: Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (<a href="http://parts.igem.org/Part:BBa_K1648005">BBa_K1648005</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.</font></p> |
<br> | <br> | ||
Line 119: | Line 119: | ||
<br> | <br> | ||
− | <p><font size="1">Figure 8. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (<a href="http://parts.igem.org/Part:BBa_K1648004">BBa_K1648004</a>) using double digestion. (A) L: DNA ladder. Lane 1-3: Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (<a href="http://parts.igem.org/Part:BBa_K1648004">BBa_K1648004</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. (B) L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (<a href="http://parts.igem.org/Part:BBa_K1648006">BBa_K1648006</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> | + | <p><font size="1.2">Figure 8. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (<a href="http://parts.igem.org/Part:BBa_K1648004">BBa_K1648004</a>) using double digestion. (A) L: DNA ladder. Lane 1-3: Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (<a href="http://parts.igem.org/Part:BBa_K1648004">BBa_K1648004</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. (B) L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (<a href="http://parts.igem.org/Part:BBa_K1648006">BBa_K1648006</a>) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. </font></p> |
<br> | <br> |
Revision as of 01:27, 19 September 2015