Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay"
| Line 57: | Line 57: | ||
<li>BBa_K525998: T7 promotor and RBS </li> | <li>BBa_K525998: T7 promotor and RBS </li> | ||
<li>BBa_C0012: lacI repressor with LVA Tag </li> | <li>BBa_C0012: lacI repressor with LVA Tag </li> | ||
| − | <li>BBa_R0010: lac | + | <li>BBa_R0010: lac Promotor </li> |
<li>BBa_K592008: lac Operator </li> | <li>BBa_K592008: lac Operator </li> | ||
</ul></br> | </ul></br> | ||
| − | <li>Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed.</li> | + | <li>Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.</li> |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 75: | Line 75: | ||
<div id="collapseTwo" class="panel-collapse collapse"> | <div id="collapseTwo" class="panel-collapse collapse"> | ||
<div class="panel-body"> | <div class="panel-body"> | ||
| − | <p> | + | <p> |
| + | <ul> | ||
| + | <li>Purification of histagged lacI</li> | ||
| + | <li>Test digest of transformed Biobricks: all clones as expected!</li> | ||
| + | <li>Midi-preps of BBa_R0010 and BBa_K592008 </li> | ||
| + | <ul> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 09:24, 9 July 2015
Plasmid Repressor Interaction Assay
- Heat shock transformation of Biobricks needed for further assays was performed. Glycerol stocks were generated and the plasmids isolated according to the kits manual. The relevant Biobricks were:
- BBa_K525998: T7 promotor and RBS
- BBa_C0012: lacI repressor with LVA Tag
- BBa_R0010: lac Promotor
- BBa_K592008: lac Operator
- Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.
- Purification of histagged lacI
- Test digest of transformed Biobricks: all clones as expected!
- Midi-preps of BBa_R0010 and BBa_K592008