Difference between revisions of "Team:Hong Kong-CUHK/insertion kit"

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<h1>Insertion Kit for Protein Expression on Magnetosome Membrane</h1>
 
<h1>Insertion Kit for Protein Expression on Magnetosome Membrane</h1>
  
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<center><img src="https://static.igem.org/mediawiki/2015/7/7a/CUHK_Project_MamC.jpg" width ="500px" style="right: 10px; margin:0px 0px -70px -10px"></center>
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<center><img src="https://static.igem.org/mediawiki/2015/7/7a/CUHK_Project_MamC.jpg" width ="500px" style="right: 10px; margin:0px 0px -100px -10px"></center>
 
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<p>Figure 1: The structure of the transmembrane mamC protein</p>
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<center><p style="font-size:14px">Figure 1: The structure of the transmembrane mamC protein</p></center>
  
 
<p>This simple construct consists of<i> mamC</i> gene, a gene coding for a transmembrane protein (1) (Figure 1) on the magnetosome membrane. Unlike usual recombinant methods to put our insert between multiple restriction sites, we put <i>mamC</i> in front of them. Now any protein we desired can be attached onto the magnetosome membrane just by fusing it with the <i>mamC</i> gene <b>by inserting it between the multiple restriction sites</b>.</p><p>(For your interest, this is done by removing the stop codon of<i> mamC</i> and the start codon of the desired protein, for example an antibody, making it a mamC-fused protein). </p>
 
<p>This simple construct consists of<i> mamC</i> gene, a gene coding for a transmembrane protein (1) (Figure 1) on the magnetosome membrane. Unlike usual recombinant methods to put our insert between multiple restriction sites, we put <i>mamC</i> in front of them. Now any protein we desired can be attached onto the magnetosome membrane just by fusing it with the <i>mamC</i> gene <b>by inserting it between the multiple restriction sites</b>.</p><p>(For your interest, this is done by removing the stop codon of<i> mamC</i> and the start codon of the desired protein, for example an antibody, making it a mamC-fused protein). </p>
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<p>Figure 2: The vector map of the transmembrane mamC protein</p>
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<center><p style="font-size:14px">Figure 2: The vector map of the transmembrane mamC protein</p><center>
 
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Revision as of 01:40, 19 September 2015

Insertion Kit for Protein Expression on Magnetosome Membrane


Figure 1: The structure of the transmembrane mamC protein

This simple construct consists of mamC gene, a gene coding for a transmembrane protein (1) (Figure 1) on the magnetosome membrane. Unlike usual recombinant methods to put our insert between multiple restriction sites, we put mamC in front of them. Now any protein we desired can be attached onto the magnetosome membrane just by fusing it with the mamC gene by inserting it between the multiple restriction sites.

(For your interest, this is done by removing the stop codon of mamC and the start codon of the desired protein, for example an antibody, making it a mamC-fused protein).

As we are now putting multiple restriction sites downstream of mamC, therefore we can insert any desire genes afterwards. Thus, we name it our insertion kit.





Figure 2: The vector map of the transmembrane mamC protein



Magnetosome + Insertion Kit = Multi-application !!!



[1].XU, Jun, et al. Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes. Frontiers in microbiology, 2014, 5.