Difference between revisions of "Team:Lethbridge HS/Results"
| Line 434: | Line 434: | ||
<p>The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.</p> | <p>The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.</p> | ||
| + | <p><b>Testing with cleaners</b></p> | ||
| + | <table class="table table-striped table-bordered bees"> <caption></caption> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Cleaner</th> | ||
| + | <th>Results</th> | ||
| + | <th>After</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>Bleach</td> | ||
| + | <td>-</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Tilex</td> | ||
| + | <td>3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Mr. Clean</td> | ||
| + | <td>-</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Fantastik</td> | ||
| + | <td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Truly</td> | ||
| + | <td>2</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Windex</td> | ||
| + | <td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Lysol</td> | ||
| + | <td>-</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>GreenWorks</td> | ||
| + | <td>1</td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
<figure style=" margin-right:20px; float:left;"> | <figure style=" margin-right:20px; float:left;"> | ||
<img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | ||
Revision as of 02:17, 19 September 2015
Results
What results did we get in the lab?
Results
Oxalic acid results:
| Oxalic Acid Concentration (mg/mL) | OD528 |
|---|---|
| 0.1 | 1.862 |
| 0.2 | 1.675 |
| 0.3 | 1.675 |
| 0.4 | 1.655 |
| 0.5 | 1.673 |
| 0.6 | 1.680 |
| 0.7 | 1.655 |
| 0.8 | 1.630 |
| 0.9 | 1.587 |
| 1.0 | 1.564 |
Bee trials:
| Treatment | Before | After |
|---|---|---|
| None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
| 0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
| 0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
| 0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
| Control | Oxalic Acid | RFP | |
|---|---|---|---|
| Parafilm Pouch | Mites dead | Mites dead | Mites dead |
| Larvae 1 | Mites dead | Mites dead | Mites dead |
| Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
| Test (Antibiotics) | Result | After |
|---|---|---|
| AMP 1 | Positive | |
| AMP 2 | Positive | Wet and slow moving. |
| CAM 1 | Positive | |
| CAM 2 | Positive | |
| TET 1 | Positive | |
| TET 2 | Positive | |
| Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
| Cleaner | Results | After |
|---|---|---|
| Bleach | - | |
| Tilex | 3 | |
| Mr. Clean | - | |
| Fantastik | 1 | |
| Truly | 2 | |
| Windex | 1 | |
| Lysol | - | |
| GreenWorks | 1 |
