Difference between revisions of "Team:UIUC Illinois/Parts"
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<h2> Part Documentation</h2> | <h2> Part Documentation</h2> | ||
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− | <li>Part | + | <li>Part: K1681000</li> |
− | <li>Part | + | <li>Part Name: PL lac O-1 SCRIBE(kanR)ON</li> |
− | <li> | + | <li>Part Type: n/a</li> |
− | <li> | + | <li>Creator: UIUC_Illinois_2015</li> |
− | <li>Short Description | + | <li>Short Description: IPTG-inducible kanamycin resistance SCRIBE cassette</li> |
− | <li>Long Description ( | + | <li>Long Description: |
− | + | SCRIBE(kanR)ON is a cassette coding for the RNA and proteins needed to restore kanamycin resistance by synthesizing multicopy single-stranded DNA (msDNA) for site-specific recombination. The part consists of the IPTG-inducible promoter PL lac O-1, a retron coding for an untranslated RNA molecule and the open reading frame for reverse transcriptase, a RBS, the open reading frame for beta recombinase, and a terminator. The reverse transcriptase uses the msDNA (msd) region of the RNA molecule as a template and the msd RNA (msr) region of the RNA molecule as a primer to synthesize the msDNA. The beta recombinase inserts the kanamycin resistance segment of the msDNA into the host’s genome during DNA replication as an Okazaki fragment.</li> | |
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− | < | + | <li>Design Considerations: |
− | + | This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters and/ or custom DNA segments for site-specific recombination.</li> | |
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Revision as of 02:31, 19 September 2015
Part Documentation
- Part: K1681000
- Part Name: PL lac O-1 SCRIBE(kanR)ON
- Part Type: n/a
- Creator: UIUC_Illinois_2015
- Short Description: IPTG-inducible kanamycin resistance SCRIBE cassette
- Long Description: SCRIBE(kanR)ON is a cassette coding for the RNA and proteins needed to restore kanamycin resistance by synthesizing multicopy single-stranded DNA (msDNA) for site-specific recombination. The part consists of the IPTG-inducible promoter PL lac O-1, a retron coding for an untranslated RNA molecule and the open reading frame for reverse transcriptase, a RBS, the open reading frame for beta recombinase, and a terminator. The reverse transcriptase uses the msDNA (msd) region of the RNA molecule as a template and the msd RNA (msr) region of the RNA molecule as a primer to synthesize the msDNA. The beta recombinase inserts the kanamycin resistance segment of the msDNA into the host’s genome during DNA replication as an Okazaki fragment.
- Design Considerations: This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters and/ or custom DNA segments for site-specific recombination.