Difference between revisions of "Team:Michigan/Safety"

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<h2>Safety in iGEM</h2>
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<h2>Safety</h2>
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Equipment and Training
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All team members performing wet lab work completed University of Michigan general lab safety training and were guided on the proper use of lab materials. Lab members operating autoclaves and centrifuges were also required to complete University of Michigan autoclave and centrifuge training respectively. Gloves and long pants were worn at all times while performing lab work, and other PPE (such as safety glasses and lab coats) were worn as appropriate. In addition to the university required safety training, all members new to biology lab work were given a hands-on, guided orientation focused on proper laboratory procedures and lab safety.
  
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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Potential Hazards and Precautions
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Our team took multiple steps to avoid potential biological and chemical hazards. We worked exclusively with BL1 materials such as e. coli. We also used GelRed (a safer alternative to ethidium bromide) for our gel imaging needs. The lab was kept clean and organized. E. Coli contaminated waste was treated with bleach before disposal, and biohazardous waste was disposed of appropriately.
  
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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Responsible Design
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Our detection system utilizes inherently benign elements such as GFP and paper. Because of this, a final product based on our system could theoretically be shipped at room temperature without special equipment or stored for long periods of time without posing a significant environmental threat. Additionally, all major components of Aptapaper (paper, DNA template, in-vitro transcription/translation materials) can be produced biologically without the need for harsh chemical synthesis.
  
  
<h4>Safe Project Design</h4>
 
  
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
 
<li>Choosing a non-pathogenic chassis</li>
 
<li>Choosing parts that will not harm humans / animals / plants</li>
 
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
<li>Including an "induced lethality" or "kill-switch" device</li>
 
</ul>
 
  
<h4>Safe Lab Work</h4>
 
  
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
<h4>Safe Shipment</h4>
 
 
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
 
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Revision as of 03:18, 19 September 2015

Safety

Equipment and Training All team members performing wet lab work completed University of Michigan general lab safety training and were guided on the proper use of lab materials. Lab members operating autoclaves and centrifuges were also required to complete University of Michigan autoclave and centrifuge training respectively. Gloves and long pants were worn at all times while performing lab work, and other PPE (such as safety glasses and lab coats) were worn as appropriate. In addition to the university required safety training, all members new to biology lab work were given a hands-on, guided orientation focused on proper laboratory procedures and lab safety. Potential Hazards and Precautions Our team took multiple steps to avoid potential biological and chemical hazards. We worked exclusively with BL1 materials such as e. coli. We also used GelRed (a safer alternative to ethidium bromide) for our gel imaging needs. The lab was kept clean and organized. E. Coli contaminated waste was treated with bleach before disposal, and biohazardous waste was disposed of appropriately. Responsible Design Our detection system utilizes inherently benign elements such as GFP and paper. Because of this, a final product based on our system could theoretically be shipped at room temperature without special equipment or stored for long periods of time without posing a significant environmental threat. Additionally, all major components of Aptapaper (paper, DNA template, in-vitro transcription/translation materials) can be produced biologically without the need for harsh chemical synthesis.