Difference between revisions of "Team:Lethbridge HS/Achievements"
| Line 249: | Line 249: | ||
<div class="col-md-12 col-sm-12"> | <div class="col-md-12 col-sm-12"> | ||
<section id="section1"> | <section id="section1"> | ||
| − | <h1 id="projecttext1" class="contentSubTitle"> | + | <h1 id="projecttext1" class="contentSubTitle">Achievements<br><small></small></h1> |
<p id="humanpractices_hp" class="bees">Our Petal Death Protein (PDP) part (BBa_K179200) was design to produce a modest amount of Oxalic acid. The PDP gene was obtained from Dianthus caryophyllus (Carnation) (Clove pink). PDP catalyzes the production of Oxalic acid from Oxaloacetate: Oxaloacetate + H2O = oxalic acid + acetate. The first 3 amino acids represent a propeptide and are not included in the PDP sequence. The PDP gene is under control of the constitutively active medium transcription promoter (J23107) and a strong RBS (B0034). This combination was chosen to allow us to tune the amount of oxalic acid produced through control of PDP production. Mutation of the transcription promoter to strong or weak activity will result in more or less PDP production and subsequent changes in oxalic acid production. The same principle can be applied to the RBS from strong to medium to weak. We tuned the oxalic acid production to achieve mite death at the lowest possible concentration while preventing any possible harm to bee health.<br> | <p id="humanpractices_hp" class="bees">Our Petal Death Protein (PDP) part (BBa_K179200) was design to produce a modest amount of Oxalic acid. The PDP gene was obtained from Dianthus caryophyllus (Carnation) (Clove pink). PDP catalyzes the production of Oxalic acid from Oxaloacetate: Oxaloacetate + H2O = oxalic acid + acetate. The first 3 amino acids represent a propeptide and are not included in the PDP sequence. The PDP gene is under control of the constitutively active medium transcription promoter (J23107) and a strong RBS (B0034). This combination was chosen to allow us to tune the amount of oxalic acid produced through control of PDP production. Mutation of the transcription promoter to strong or weak activity will result in more or less PDP production and subsequent changes in oxalic acid production. The same principle can be applied to the RBS from strong to medium to weak. We tuned the oxalic acid production to achieve mite death at the lowest possible concentration while preventing any possible harm to bee health.<br> | ||
</p> | </p> | ||
| Line 263: | Line 263: | ||
<p> | <p> | ||
| − | Bronze: <br><br> | + | <b>Bronze:</b> <br><br> |
Our team successfully registered for iGEM. <br> | Our team successfully registered for iGEM. <br> | ||
We had a great deal of fun during the summer and can’t wait to attend the Jamboree!<br> | We had a great deal of fun during the summer and can’t wait to attend the Jamboree!<br> | ||
| Line 273: | Line 273: | ||
| − | Silver:<br><br> | + | <b>Silver:</b><br><br> |
We submitted the following parts to the iGEM Registry:BBa_K1792002 (Dextranase), and BBa_K1792004 (Nuclease).<br> | We submitted the following parts to the iGEM Registry:BBa_K1792002 (Dextranase), and BBa_K1792004 (Nuclease).<br> | ||
| Line 280: | Line 280: | ||
| − | Gold:<br><br> | + | <b>Gold:</b><br><br> |
We created an innovative human practices activity by creating a petition about amending the urban beekeeping bylaw in our community and distributing “seed bombs” to the public to encourage the growth of bee-friendly places within our city.<br> | We created an innovative human practices activity by creating a petition about amending the urban beekeeping bylaw in our community and distributing “seed bombs” to the public to encourage the growth of bee-friendly places within our city.<br> | ||
We hosted a workshop at the University of Lethbridge where the Central Calgary High School team, Consort Alberta High School team, and the OLS Canmore High School team were taught basic laboratory techniques, such as pipetting, miniprepping, and ligating that are essential to the lab component of iGEM.<br><br> | We hosted a workshop at the University of Lethbridge where the Central Calgary High School team, Consort Alberta High School team, and the OLS Canmore High School team were taught basic laboratory techniques, such as pipetting, miniprepping, and ligating that are essential to the lab component of iGEM.<br><br> | ||
Revision as of 03:28, 19 September 2015
Project
Achievements
Achievements
Our Petal Death Protein (PDP) part (BBa_K179200) was design to produce a modest amount of Oxalic acid. The PDP gene was obtained from Dianthus caryophyllus (Carnation) (Clove pink). PDP catalyzes the production of Oxalic acid from Oxaloacetate: Oxaloacetate + H2O = oxalic acid + acetate. The first 3 amino acids represent a propeptide and are not included in the PDP sequence. The PDP gene is under control of the constitutively active medium transcription promoter (J23107) and a strong RBS (B0034). This combination was chosen to allow us to tune the amount of oxalic acid produced through control of PDP production. Mutation of the transcription promoter to strong or weak activity will result in more or less PDP production and subsequent changes in oxalic acid production. The same principle can be applied to the RBS from strong to medium to weak. We tuned the oxalic acid production to achieve mite death at the lowest possible concentration while preventing any possible harm to bee health.
Bronze:
Our team successfully registered for iGEM.
We had a great deal of fun during the summer and can’t wait to attend the Jamboree!
All of the required forms, including the judging and consent forms, were completed and sent in.
We completed a description of our project, which can be viewed on our team wiki. Our parts were submitted to the iGEM registry.
We included an attributions page on our wiki.
We created a poster for our project and it will be presented during the poster session at the Jamboree.
Our iGEM team was mentored by the Collegiate Team at the University of Lethbridge. They helped us with procedures and in designing our constructs.In addition to the Collegiate team, we hosted a high school iGEM workshop in February to help other iGEM teams in Alberta learn laboratory skills that they would need.
Silver:
We submitted the following parts to the iGEM Registry:BBa_K1792002 (Dextranase), and BBa_K1792004 (Nuclease).
We characterized our BBa_K1792000 (PDP) part.
We completed many human practice endeavors for our projects.
Gold:
We created an innovative human practices activity by creating a petition about amending the urban beekeeping bylaw in our community and distributing “seed bombs” to the public to encourage the growth of bee-friendly places within our city.
We hosted a workshop at the University of Lethbridge where the Central Calgary High School team, Consort Alberta High School team, and the OLS Canmore High School team were taught basic laboratory techniques, such as pipetting, miniprepping, and ligating that are essential to the lab component of iGEM.