Difference between revisions of "Team:Lethbridge HS/Results"
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| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/5/55/LetHS_2015_PDPConstructAcidProduction.png" width=600px height=400px class="img-responsive bees"> | <img src="https://static.igem.org/mediawiki/2015/5/55/LetHS_2015_PDPConstructAcidProduction.png" width=600px height=400px class="img-responsive bees"> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="biofilms"> |
<img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | ||
<figcaption>Biofilms fluorescent imaging under Typhoon</figcaption> | <figcaption>Biofilms fluorescent imaging under Typhoon</figcaption> | ||
</figure> | </figure> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg" width=400px height=300px class="img-responsive"> | ||
<figcaption>Different concentration of oxalic acid 3 minutes into incubation time at RT</figcaption> | <figcaption>Different concentration of oxalic acid 3 minutes into incubation time at RT</figcaption> | ||
</figure> | </figure> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png" width=400px height=300px class="img-responsive"> | ||
<figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
</figure> | </figure> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg" width=400px height=300px class="img-responsive"> | ||
<figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
</figure> | </figure> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png" width=400px height=300px class="img-responsive"> | ||
<figcaption>Microcentrifuge tube containing Honey Bee</figcaption> | <figcaption>Microcentrifuge tube containing Honey Bee</figcaption> | ||
</figure> | </figure> | ||
| − | <figure style=" margin-right:20px; float:left;"> | + | <figure style=" margin-right:20px; float:left;" class="bees"> |
<img src="https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png" width=400px height=300px class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png" width=400px height=300px class="img-responsive"> | ||
<figcaption>Microcentrifuge tube containing Varroa mites</figcaption> | <figcaption>Microcentrifuge tube containing Varroa mites</figcaption> | ||
Revision as of 03:45, 19 September 2015
Results
What results did we get in the lab?
Results
Oxalic acid results:
| Oxalic Acid Concentration (mg/mL) | OD528 |
|---|---|
| 0.1 | 1.862 |
| 0.2 | 1.675 |
| 0.3 | 1.675 |
| 0.4 | 1.655 |
| 0.5 | 1.673 |
| 0.6 | 1.680 |
| 0.7 | 1.655 |
| 0.8 | 1.630 |
| 0.9 | 1.587 |
| 1.0 | 1.564 |
Bee trials:
| Treatment | Before | After |
|---|---|---|
| None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
| 0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
| 0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
| 0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
| Control | Oxalic Acid | RFP | |
|---|---|---|---|
| Parafilm Pouch | Mites dead | Mites dead | Mites dead |
| Larvae 1 | Mites dead | Mites dead | Mites dead |
| Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
| Test (Antibiotics) | Result | After |
|---|---|---|
| AMP 1 | Positive | |
| AMP 2 | Positive | Wet and slow moving. |
| CAM 1 | Positive | |
| CAM 2 | Positive | |
| TET 1 | Positive | |
| TET 2 | Positive | |
| Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
| Cleaner | Results | After |
|---|---|---|
| Bleach | - | |
| Tilex | 3 | |
| Mr. Clean | - | |
| Fantastik | 1 | |
| Truly | 2 | |
| Windex | 1 | |
| Lysol | - | |
| GreenWorks | 1 |
