Difference between revisions of "Team:Peking/Description"

 
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<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
−
  <!-- Define Charset -->
 
 
   <meta charset="utf-8">
 
   <meta charset="utf-8">
−
  <!-- Responsive Metatag -->
+
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-
−
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
+
 
−
    <!-- Page Description and Author -->
+
scale=1">
 
   <meta name="Peking-iGEM-2015" content="iGEM - Peking iGEM 2015">
 
   <meta name="Peking-iGEM-2015" content="iGEM - Peking iGEM 2015">
−
 
 
 
   <!--add  ?action=raw&amp;ctype=text/css    when using Css-->
 
   <!--add  ?action=raw&amp;ctype=text/css    when using Css-->
−
  <!-- Bootstrap CSS V  -->
+
   <link rel="stylesheet" href="https://2015.igem.org/Template:Peking/new?
−
   <link rel="stylesheet" href="https://2015.igem.org/Template:Peking/CSS?action=raw&amp;ctype=text/css" type="text/css" media="screen">
+
 
−
   <!-- Font Awesome CSS V -->
+
action=raw&amp;ctype=text/css" type="text/css" media="screen">
−
  <!--<link rel="stylesheet" href="https://2015.igem.org/Template:Peking/CSS/Font?action=raw&amp;ctype=text/css" type="text/css" media="screen">-->
+
   <link rel="stylesheet" type="text/css"  
−
  <!-- Margo CSS Styles  V-->
+
 
−
  <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Style?action=raw&amp;ctype=text/css" media="screen">
+
href="https://2015.igem.org/Template:Peking/Style/new?
−
  <!-- Responsive CSS Styles  -->
+
 
−
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Responsive?action=raw&amp;ctype=text/css" media="screen">
+
action=raw&amp;ctype=text/css" media="screen">
−
  <!-- Css3 Transitions Styles  -->
+
   <link rel="stylesheet" type="text/css"  
−
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Animate?action=raw&amp;ctype=text/css" media="screen">
+
 
−
  <!-- Color CSS Styles  -->
+
href="https://2015.igem.org/Template:Peking/Responsive/new?
−
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue?action=raw&amp;ctype=text/css " title="blue" media="screen" />
+
 
−
 
+
action=raw&amp;ctype=text/css" media="screen">
−
   <style type="text/css">
+
   <link rel="stylesheet" type="text/css"  
−
      /*get rid of the annoying iGEM style*/
+
 
−
         #content{
+
href="https://2015.igem.org/Template:Peking/Animate/new?
−
            padding:0;
+
 
−
            border:0;
+
action=raw&amp;ctype=text/css" media="screen">
−
            width:100%;
+
   <link rel="stylesheet" type="text/css"  
−
        }
+
 
−
       .firstHeading{
+
href="https://2015.igem.org/Template:Peking/Blue/new?
−
        visibility:hidden;
+
 
−
      }
+
action=raw&amp;ctype=text/css " title="blue" media="screen" />
−
       h2{
+
   <style type="text/css">/*get rid of the annoying iGEM style*/
−
        border-bottom:none;
+
         #top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -
−
        padding-bottom:.5em;
+
 
−
       
+
webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-
−
      }     
+
 
−
        .carousel-control.left, .carousel-control.right {
+
box;}
−
          background-image: none
+
        #top_menu_under{font-size: 12px;}
−
        }
+
        #top_menu_14{font-size: 12px; height:18px}
−
     /* to style the team page*/
+
        #top_menu_inside li{font-size: 12px;}
−
        .individual-picture{
+
        #user_item{font-size: 12px;}
−
        max-width:200px;margin-top:5px; margin-bottom:10px;
+
      #content{padding:0;border:0;width:100%;}
−
        }
+
       .firstHeading{visibility:hidden;}
 +
       h2{border-bottom:none;padding-bottom:.5em;}     
 +
      .carousel-control.left, .carousel-control.right{background-image: none}
 +
     /* to style the sidebar*/
 
         @media (min-width:1024px){
 
         @media (min-width:1024px){
−
       #sidebar1{position:relative;top:120px;}
+
       #sidebar1{position:relative;top:120px;max-width:200px;}
 
       #sidebar2{display:none;}
 
       #sidebar2{display:none;}
 
       #nav-top{height:88px;}
 
       #nav-top{height:88px;}
−
 
 
     }
 
     }
 
       @media (max-width: 1023px){
 
       @media (max-width: 1023px){
 
       #sidebar1{display:none;}
 
       #sidebar1{display:none;}
 
       #sidebar2{display:block;}
 
       #sidebar2{display:block;}
−
 
 
     }
 
     }
−
        
+
    .back-to-top{z-index:9999;}
−
     </style>
+
       ul.dropdown{margin-left: 0px; margin-top: 0px;}
−
      
+
    .classic-title span {
 +
     position: absolute;
 +
     margin-top: -30px;
 +
    border-bottom-color: #00afd1;
 +
}
 +
.page-banner{
 +
  background-color:#e0e0e0;margin-top:1%;margin-
  
−
  <!--[if IE 8]><script src="http://html5shiv.googlecode.com/svn/trunk/html5.js"></script><![endif]-->
+
bottom:20px;padding:20px;padding-top:40px;
−
  <!--[if lt IE 9]><script src="http://html5shiv.googlecode.com/svn/trunk/html5.js"></script><![endif]-->
+
}
−
 
+
 
 +
    </style>
 
</head>
 
</head>
 +
 
<body>
 
<body>
 
   <!-- Container -->
 
   <!-- Container -->
 
   <div id="container">
 
   <div id="container">
−
   
+
    <!-- Start Header -->
−
    <!-- Start Header -->
+
−
    <div class="hidden-header"></div>
+
 
     <header class="clearfix">
 
     <header class="clearfix">
−
         
+
         <!-- Start  Logo &amp; Naviagtion  -->
−
         <!-- Start  Logo & Naviagtion  -->
+
 
             <div class="navbar navbar-default navbar-top">
 
             <div class="navbar navbar-default navbar-top">
 
                 <div class="container">
 
                 <div class="container">
 
                     <div class="navbar-header" >
 
                     <div class="navbar-header" >
 
                         <!-- Stat Toggle Nav Link For Mobiles -->
 
                         <!-- Stat Toggle Nav Link For Mobiles -->
−
                         <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse" style="marginn-top:20px">
+
                         <button type="button" class="navbar-toggle" data-
−
                             <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
+
 
 +
toggle="collapse" data-target=".navbar-collapse" style="margin-top:40px">
 +
                             <i class="fa"><img  style="margin-
 +
 
 +
left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-
 +
 
 +
1.png" ></i>
 
                         </button>
 
                         </button>
 
                         <!-- End Toggle Nav Link For Mobiles -->
 
                         <!-- End Toggle Nav Link For Mobiles -->
 
                         <a class="navbar-brand" href="https://2015.igem.org/Team:Peking">
 
                         <a class="navbar-brand" href="https://2015.igem.org/Team:Peking">
−
                             <img alt="Peking iGEM 2015" src="https://static.igem.org/mediawiki/2015/8/8b/Peking-header-logo-1.png" style="height:55px;margin-top:7px">
+
                             <img alt="Peking iGEM 2015"  
 +
 
 +
src="https://static.igem.org/mediawiki/2015/8/8b/Peking-header-logo-1.png"  
 +
 
 +
style="height:55px;margin-top:7px">
 
                         </a>
 
                         </a>
 
                     </div>
 
                     </div>
−
                     <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
+
                     <div class="navbar-collapse collapse"  style="padding-
−
                       
+
 
 +
top:5px;padding-bottom:0">
 
                         <!-- Start Navigation List -->
 
                         <!-- Start Navigation List -->
−
                         <ul class="nav navbar-nav navbar-right " id="nav-top" style="padding-bottom:15px;padding-top:10px">
+
                         <ul class="nav navbar-nav navbar-right " style="padding-
 +
 
 +
bottom:15px;height:88px;padding-top:10px">
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking">Home</a>
+
                                 <a href="https://2015.igem.org/Team:Peking/JudgingCriteria"  
−
                            </li>
+
 
−
              <li>
+
class="active">Achievements</a>
−
                                <a class="active" href="https://2015.igem.org/Team:Peking/JudgingCriteria">Achievements</a>
+
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a class="active" href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
+
                                     <li><a class="active"  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking/Design">Project</a>
+
                                 <a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Design">Project</a>
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Design">Overview</a>
 
                                     </li>
 
                                     </li>
−
<li><a href="#">Background</a>
+
<li><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired<span
 +
 
 +
style="text-transform:lowercase"> d</span>Cas9 Reporter</a>                               
 +
 
 +
 
 +
</li>
 +
                                    <li><a
 +
 
 +
href="https://2015.igem.org/Team:Peking/Design/Isothermal">Iso-<span
 +
 
 +
style="text-transform:lowercase">t</span>hermal Amplification</a></li>
 +
                                    <li><a
 +
 
 +
href="https://2015.igem.org/Team:Peking/Device">Hardware</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">CRIPSR</a>
+
                                     <li><a  
−
                                    </li>
+
 
−
                                    <li><a href="#">Isothermal PCR</a>
+
href="https://2015.igem.org/Team:Peking/Design/Speculation">Speculation</a>
−
                                    </li>
+
−
                                    <li><a href="#">B. subtillis</a>
+
−
                                    </li>
+
−
                                    <li><a href="#">Device</a>
+
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking/Modeling">Modelling</a>
+
                                 <a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Modeling">Modeling</a>
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="#">Link 1</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Modeling">Array Design</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 2</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Modeling/Analysis">Analysis
 +
 
 +
algorithm</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking/Practices">Human Practice</a>
+
                                 <a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices">Practices</a>
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="#">Link 1</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices">Overview</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 2</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices/Background">TB Facts</a>
 +
                                    </li>
 +
                                    <li><a 
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices/Consultation">Consultation and
 +
 
 +
Interview</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 3</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices/Engagement">Public
 +
 
 +
Engagement</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 4</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Practices/Outreach">Ethics and Economics
 +
 
 +
Issues</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
Line 146: Line 209:
 
                                 <a href="https://2015.igem.org/Team:Peking/Team">Lab</a>
 
                                 <a href="https://2015.igem.org/Team:Peking/Team">Lab</a>
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Team">Team</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Team">Team</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Notebook">Notebook</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Notebook">Notebook</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Attributions">Attributions</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Attributions">Attributions</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="https://2015.igem.org/Team:Peking/Safety">Safety</a>
+
                                     <li><a  
 +
 
 +
href="https://2015.igem.org/Team:Peking/Safety">Safety</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
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                 </div>
 
                 </div>
 
             </div>
 
             </div>
−
             <!-- End Header Logo & Naviagtion -->
+
             <!-- End Header Logo &amp; Naviagtion -->
 
              
 
              
 
         </header>  
 
         </header>  
Line 168: Line 239:
 
      
 
      
 
     <!-- Start Page Banner -->
 
     <!-- Start Page Banner -->
−
     <div class="page-banner" style="background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;">  
+
     <div class="page-banner">  
 
       <div class="container">
 
       <div class="container">
 
         <div class="row">
 
         <div class="row">
 
           <div class="col-md-6">
 
           <div class="col-md-6">
−
             <h2><b>Parts</b></h2>
+
             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span
−
             <p>Each part give us a new experience.</p>
+
 
 +
style="text-transform:lowercase">arts</span></h2>
 +
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part give us a  
 +
 
 +
new experience.</p>
 
           </div>
 
           </div>
 
           <div class="col-md-6">
 
           <div class="col-md-6">
 
             <ul class="breadcrumbs">
 
             <ul class="breadcrumbs">
 
               <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
 
               <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
−
               <li>Achievements</li>
+
               <li>Parts</li>
 
             </ul>
 
             </ul>
 
           </div>
 
           </div>
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     <!-- End Page Banner -->
 
     <!-- End Page Banner -->
  
 +
 
     <!-- Start Content -->
 
     <!-- Start Content -->
 
     <div id="content">
 
     <div id="content">
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                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Parts"
 +
 
 +
style="color:#00afd1">Parts Document</a>
 
                 </li>
 
                 </li>
 +
               
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts  
−
                </li>
+
 
−
                <li>
+
Collection</a>
−
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
+
−
                </li>
+
−
                <li>
+
−
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
+
−
                </li>
+
−
                <li>
+
−
                  <a href="https://2015.igem.org/Team:Peking/Description"  style="color:#00afd1">Parts Contribution</a>
+
 
                 </li>
 
                 </li>
 +
             
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
 +
 
             <div id="sidebar2"class="widget widget-categories">
 
             <div id="sidebar2"class="widget widget-categories">
−
               <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
+
               <h4>Parts <span class="head-line"></span></h4>
 
                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Parts"
 +
 
 +
style="color:#00afd1">Parts Document</a>
 
                 </li>
 
                 </li>
 +
               
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts  
−
                </li>
+
 
−
                <li>
+
Collection</a>
−
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
+
−
                </li>
+
−
                <li>
+
−
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
+
−
                </li>
+
−
                <li>
+
−
                  <a href="https://2015.igem.org/Team:Peking/Description"  style="color:#00afd1">Parts Contribution</a>
+
 
                 </li>
 
                 </li>
 +
             
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
Line 238: Line 309:
  
 
           <!-- Page Content -->
 
           <!-- Page Content -->
−
           <div class="col-md-9 page-content">
+
           <div class="col-md-9 page-content" style="text-align:justify;">
−
             <div id="Basic_Part">
+
             <div id="Part-Favorite part" id="Document">
 
             <!-- Classic Heading -->
 
             <!-- Classic Heading -->
−
               <h3 class="classic-title"><span>Part Contribution</span></h3>
+
               <h3 class="classic-title" style="margin-top:50px"><span>Favorite
−
              <h4 style="margin-bottom:15px;"><span><a href="" style="color:#00afd1">Part Contribution 1</a></span></h4>
+
−
              <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="max-width:500px">
+
−
              <p><b> Fig.1 At vero eos et</b></p>
+
−
              <p>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.</p>
+
  
−
                <div class="hr5" style="margin-top:25px;margin-bottom:45px"></div>
+
Parts</span></h3>
 +
<div>
 +
              <p><b>Nluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010"
  
−
              <h4 style="margin-bottom:15px"><span><a href="" style="color:#00afd1">Part Contribution 2</a></span></h4>
+
style="color:#00afd1">(BBa_K1689010)</a></p>
−
               <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="max-width:500px">
+
              <div class="row">
−
              <p><b> Fig.2 At vero eos et</b></p>
+
               
−
              <p>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.</p>
+
                  <div class="col-md-8">
 +
               <img src="https://static.igem.org/mediawiki/2015/a/a2/Peking-Parts-ND-
  
−
              <div class="hr5" style="margin-top:25px;margin-bottom:45px"></div>
+
long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-2"></div>
 +
          </div>
 +
              <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9
  
−
              <h4 style="margin-bottom:15px"><span><a href="" style="color:#00afd1">Part Contribution 3</a></span></h4>
+
fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with
−
              <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="max-width:500px">
+
−
              <p><b> Fig.2 At vero eos et</b></p>
+
−
              <p>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.</p>
+
  
−
              <h3 class="classic-title"><span>References</span></h3>
+
Cluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?
−
              <p> [1] At vero eos et accusamus et iusto odio dignissimos ducimus qui 
+
−
              <br> [2] At vero eos et accusamus et iusto odio dignissimos ducimus qui
+
−
              <br> [3] At vero eos et accusamus et iusto odio dignissimos ducimus qui
+
−
              <br> [4] At vero eos et accusamus et iusto odio dignissimos ducimus qui
+
−
              <br> [5] At vero eos et accusamus et iusto odio dignissimos ducimus qui
+
−
            </p>
+
  
 +
title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a> and sgRNAs,
 +
 +
our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>Cluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009"
 +
 +
style="color:#00afd1">(BBa_K1689009)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/3/32/Peking-Parts-CD-
 +
 +
long.png" style="max-height:200px">
 
             </div>
 
             </div>
−
              
+
             <div class="col-md-4"></div>
−
</div>
+
          </div>
−
</div>
+
              <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9
 +
 
 +
fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with
 +
 
 +
Nluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a> and sgRNAs,
 +
 
 +
our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>sgRNA generator</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000"
 +
 
 +
style="color:#00afd1">(BBa_K1689000)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/f/f2/Peking-Parts-gRNA-
 +
 
 +
long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
              <p> sgRNA generator is used for generating sgRNA which recognizes
 +
 
 +
targeted DNA, then guiding dCas9 to it.
 +
</p>
 +
             
 +
              </div>
 +
 
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part
 +
 
 +
List</span></h3>
 +
<!--<div>
 +
<div id='groupparts' style='min-height:100px;width:700px;'><div
 +
 
 +
style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid
 +
 
 +
gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi',
 +
 
 +
{ t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();}
 +
 
 +
);</script>-->
 +
<div class="col-md-12">
 +
  <table class="table table-hover table-striped" style="color:#666;font-size:14px">
 +
<thead>
 +
<tr>
 +
<th>Favorite</th>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Design</th>
 +
</tr>
 +
</thead>
 +
 
 +
<tbody>
 +
<tr>
 +
  <td>&#160;&#8730;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000"
 +
 
 +
style="color:#00afd1">BBa_K1689000</a></td>
 +
<td>Composite</td>
 +
<td>sgRNA generator</td>
 +
<td>ZHAO Xuejin</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;&#8730;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008"
 +
 
 +
style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>Coding sequence of dCas9-Nluc</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;&#8730;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009"
 +
 
 +
style="color:#00afd1">BBa_K1689009</a></td>
 +
<td>Composite</td>
 +
<td>Coding sequence Cluc-dCas9</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010"
 +
 
 +
style="color:#00afd1">BBa_K1689010</a></td>
 +
<td>Compostie</td>
 +
<td>Coding sequence of N-luc-dCas9</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001"
 +
 
 +
style="color:#00afd1">BBa_K1689001</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of STV-N-luc</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002"
 +
 
 +
style="color:#00afd1">BBa_K1689002</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Cluc-STV</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003"
 +
 
 +
style="color:#00afd1">BBa_K1689003</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc416-FRB</td>
 +
<td>Xiang Li</td> 
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004"
 +
 
 +
style="color:#00afd1">BBa_K1689004</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc398-FRB</td>
 +
<td>Xiang Li</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005"
 +
 
 +
style="color:#00afd1">BBa_K1689005</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc398</td>
 +
<td>Xiang Li</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&#160;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006"
 +
 
 +
style="color:#00afd1">BBa_K1689006</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc394</td>
 +
<td>Xiang Li</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007"
 +
 
 +
style="color:#00afd1">BBa_K1689007</a></td>
 +
<td>Composite</td>
 +
<td>Coding sequence of dCas9-C-luc</td>
 +
<td>ZHANG Yihao</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011"
 +
 
 +
style="color:#00afd1">BBa_K1689011</a></td>
 +
<td>Composite</td>
 +
<td>delta alpha segment of lacz fused with dCas9, His tag allows for protein
 +
 
 +
purification</td>
 +
<td>Jiaofeng Li</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012"
 +
 
 +
style="color:#00afd1">BBa_K1689012</a></td>
 +
<td>Composite</td>
 +
<td>N terminal of β-lactamase fused with dCas9</td>
 +
<td>Jiaofeng Li</th>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013"
 +
 
 +
style="color:#00afd1">BBa_K1689013</a></td>
 +
<td>Compostie</td>
 +
<td>NFluc of lactamase fused with dCas9, His tag allows for protein
 +
 
 +
purification</td>
 +
<td>Jiaofeng Li</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014"
 +
 
 +
style="color:#00afd1">BBa_K1689014</a></td>
 +
<td>Coding</td>
 +
<td>dCas9 fused with C terminal of beta-lactamase</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015"
 +
 
 +
style="color:#00afd1">BBa_K1689015</a></td>
 +
<td>Coding</td>
 +
<td>segment F[1,2] of DHFR-dCas9 fusion protein</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016"
 +
 
 +
style="color:#00afd1">BBa_K1689016</a></td>
 +
<td>Coding</td>
 +
<td>fusion protein of dCas9-F[1,2]</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017"
 +
 
 +
style="color:#00afd1">BBa_K1689017</a></td>
 +
<td>Coding</td>
 +
<td>DHFR F3 fragment-dCas9 fusion protein</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018"
 +
 
 +
style="color:#00afd1">BBa_K1689018</a></td>
 +
<td>Coding</td>
 +
<td>dCas9 fused with F3</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019"
 +
 
 +
style="color:#00afd1">BBa_K1689019</a></td>
 +
<td>Coding</td>
 +
<td>Δα segment of β-galactosidase fused with dCas9</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&#160;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020"
 +
 
 +
style="color:#00afd1">BBa_K1689020</a></td>
 +
<td>Coding</td>
 +
<td>dCas9 fused with delta omega segment of β-galactosidase</td>
 +
<td>Wei Weijia</td>
 +
</tr>
 +
</table>  
 
</div>
 
</div>
 +
 +
                <p><b>Parts for paired dCas9 reporter system</b></p>
 +
 +
                <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?
 +
 +
title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-
 +
 +
Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005"
 +
 +
style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004"
 +
 +
style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006"
 +
 +
style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the
 +
 +
reconsitituation activity of split luciferase.</p>
 +
                <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?
 +
 +
title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-
 +
 +
dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009"
 +
 +
style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008"
 +
 +
style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007"
 +
 +
style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for
 +
 +
constructing paired dCas9 reporter system.</p>
 +
                <p>&#916;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 +
title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9
 +
 +
-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012"
 +
 +
style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014"
 +
 +
style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014"
 +
 +
style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015"
 +
 +
style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016"
 +
 +
style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017"
 +
 +
style="color:#00afd1">(BBa_K1689017)</a>,<br/>  dCas9-F3 <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018"
 +
 +
style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&#916;&#945;<a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019"
 +
 +
style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &#916;&#969;-dCas9 <a
 +
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020"
 +
 +
style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development
 +
 +
of dectection.</p>
 +
                <p><b>Parts for Molecular Beacon</b></p>
 +
                <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?
 +
 +
title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-
 +
 +
STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002"
 +
 +
style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular
 +
 +
beacon.</p>
 
</div>
 
</div>
 +
                <p id="Collection"><b>Parts for sgRNA generation</b></p>
 +
<div>
 +
                <p>sgRNA generator <a href="http://parts.igem.org/wiki/index.php?
 +
 +
title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a> is used for
 +
 +
generating sgRNA</p>
 +
             
 
</div>
 
</div>
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part
  
−
        <!--FOOTER************FOOTER*******************FOOTER***************FOOTER************-->
+
Collection</span></h3>
−
<!--******************************* the only thing to CHANGE is the SHARE LINK******************************-->
+
<div>
 +
                <p>2015 Peking iGEM has not only transformed the sequence
 +
 
 +
information into detectable bioluminescence, but also explore a wide range in which
 +
 
 +
Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split
 +
 
 +
enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the 
 +
 
 +
form of read-out will be abundant at the same time. That is, our PC reporter system
 +
 
 +
has successfully provided a platform to produce a variety of signals.</p>
 +
                <p>We combine the specific sequence binding activity of dCas9 with
 +
 
 +
diverse characteristics of split enzymes, thus creating a part collection named "PC
 +
 
 +
Reporters Collection".</p>
 +
                <p>The Collection includes:</p>
 +
 
 +
                <p><b>dCas9-split luciferase Parts</b></p>
 +
                <p>Nluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a><br/>
 +
                Cluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a><br/>
 +
                dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a><br/>
 +
                dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a></p>
 +
 
 +
                <p><b>dCas9-split Dihydrofolate Reductase Parts</b></p>
 +
                <p>F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a><br/>
 +
                F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689017)</a><br/>
 +
                dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a><br/>
 +
                dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a></p>
 +
 
 +
                <p><b>dCas9-split β–Lactamase Parts</b></p>
 +
                <p>dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a><br/>
 +
                Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689013" style="color:#00afd1">(BBa_K1689013)</a><br/>
 +
                dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a></p>
 +
               
 +
                <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
 +
                <p>&#916;&#945;-dCas9 <a
 +
 
 +
href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011"
 +
 
 +
style="color:#00afd1">(BBa_K1689011)</a><br/>
 +
                dCas9-&#916;&#945; <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
 +
                &#916;&#969;-dCas9 <a href="http://parts.igem.org/wiki/index.php?
 +
 
 +
title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
 +
                <p>Among them, the PC reporters with split luciferase oxidizes luciferin
 +
 
 +
and gives out bioluminescence signal, while the other three reporters take electric
 +
 
 +
signal as their output, which is also able to be detected and qualified.</p>
 +
                </div>
 +
 
 +
                </div>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
                </div>
 +
 
 +
            <!--divider-->
 +
           
 +
 
 +
   
 +
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 +
 
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Latest revision as of 03:52, 19 September 2015

Peking iGEM 2015

Parts

Each part give us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator ZHAO Xuejin
 √ BBa_K1689008 Composite Coding sequence of dCas9-Nluc ZHANG Yihao
 √ BBa_K1689009 Composite Coding sequence Cluc-dCas9 ZHANG Yihao
  BBa_K1689010 Compostie Coding sequence of N-luc-dCas9 ZHANG Yihao
  BBa_K1689001 Coding Coding sequence of STV-N-luc ZHANG Yihao
  BBa_K1689002 Coding Coding sequence of Cluc-STV ZHANG Yihao
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB Xiang Li
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB Xiang Li
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 Xiang Li
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 Xiang Li
  BBa_K1689007 Composite Coding sequence of dCas9-C-luc ZHANG Yihao
  BBa_K1689011 Composite delta alpha segment of lacz fused with dCas9, His tag allows for protein purification Jiaofeng Li
  BBa_K1689012 Composite N terminal of β-lactamase fused with dCas9 Jiaofeng Li
  BBa_K1689013 Compostie NFluc of lactamase fused with dCas9, His tag allows for protein purification Jiaofeng Li
  BBa_K1689014 Coding dCas9 fused with C terminal of beta-lactamase Wei Weijia
  BBa_K1689015 Coding segment F[1,2] of DHFR-dCas9 fusion protein Wei Weijia
  BBa_K1689016 Coding fusion protein of dCas9-F[1,2] Wei Weijia
  BBa_K1689017 Coding DHFR F3 fragment-dCas9 fusion protein Wei Weijia
  BBa_K1689018 Coding dCas9 fused with F3 Wei Weijia
  BBa_K1689019 Coding Δα segment of β-galactosidase fused with dCas9 Wei Weijia
  BBa_K1689020 Coding dCas9 fused with delta omega segment of β-galactosidase Wei Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP- Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc- dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9 -Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc- STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explore a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.