Difference between revisions of "Team:Waterloo"
m |
|||
| Line 25: | Line 25: | ||
</p> | </p> | ||
<p> | <p> | ||
| − | <strong>Simple sgRNA Exchange</strong>: We’re making it easy to test different sgRNA designs: restriction sites added to the sgRNA backbone allow 20 nucleotide target sequences to be swapped without excessive cloning. | + | <strong class="bluetext">Simple sgRNA Exchange</strong>: We’re making it easy to test different sgRNA designs: restriction sites added to the sgRNA backbone allow 20 nucleotide target sequences to be swapped without excessive cloning. |
</p> | </p> | ||
<p> | <p> | ||
| − | <strong>Cas9 PAM Flexibility</strong>: We’re applying recent research on viable mutations within Cas9’s PAM-interacting domain to design (d)Cas9 variants that bind to novel PAM sites, moving towards the goal of a suite of variants that can bind any desired sequence. We believe our re-engineered CRISPR-Cas9 will give biologists increased ability to optimize targeting in many applications. | + | <strong class="orangetext">Cas9 PAM Flexibility</strong>: We’re applying recent research on viable mutations within Cas9’s PAM-interacting domain to design (d)Cas9 variants that bind to novel PAM sites, moving towards the goal of a suite of variants that can bind any desired sequence. We believe our re-engineered CRISPR-Cas9 will give biologists increased ability to optimize targeting in many applications. |
</p> | </p> | ||
<p> | <p> | ||
| − | <strong>CRISPR Plant Defense</strong>: The application we chose to explore is a proof-of-concept antiviral system defending the model plant <i>Arabidopsis thaliana</i> against Cauliflower Mosaic Virus, which would benefit from testing a large number of possible sgRNAs in the viral genome. | + | <strong class="greentext">CRISPR Plant Defense</strong>: The application we chose to explore is a proof-of-concept antiviral system defending the model plant <i>Arabidopsis thaliana</i> against Cauliflower Mosaic Virus, which would benefit from testing a large number of possible sgRNAs in the viral genome. |
</p> | </p> | ||
Revision as of 18:50, 1 October 2015
Simple sgRNA Exchange
Cas9 PAM Flexibility
CRISPR Plant Defense
Re-engineering CRISPR-Cas9 with functional applications in eukaryotic systems
CRISPR-Cas9 is an exciting tool for synthetic biologists because it can target and edit genomes with unprecedented specificity. Our team is attempting to re-engineer CRISPR to make it more flexible and easier to use.
Simple sgRNA Exchange: We’re making it easy to test different sgRNA designs: restriction sites added to the sgRNA backbone allow 20 nucleotide target sequences to be swapped without excessive cloning.
Cas9 PAM Flexibility: We’re applying recent research on viable mutations within Cas9’s PAM-interacting domain to design (d)Cas9 variants that bind to novel PAM sites, moving towards the goal of a suite of variants that can bind any desired sequence. We believe our re-engineered CRISPR-Cas9 will give biologists increased ability to optimize targeting in many applications.
CRISPR Plant Defense: The application we chose to explore is a proof-of-concept antiviral system defending the model plant Arabidopsis thaliana against Cauliflower Mosaic Virus, which would benefit from testing a large number of possible sgRNAs in the viral genome.