Difference between revisions of "Team:SVCE Chennai/Notebook"

APRIL 2015

• Recruitment of team members
• Setting up of lab with necessary equipments and chemicals

• Brainstorming

MAY- MID JUNE 2015

• Brainstorming
• University exams hence no lab work

MID JUNE- JULY 2015

• lab practice sessions
• confirmation of project title

AUGUST 2015

Week 1

• Submitted safety forms and also regarding our project forms.

• Preparation of LB broth and LB Agar and plates were poured.
• Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.
• Abstract and title of our project was uploaded.

• Prepared chemicals and buffers for plasmid isolation.
• FtsZ was innoculated in LB broth.
• Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.

• Sequences for gBlocks were constructed for ordering.

Week 2:

• Plasmid isolation was performed for protocol optimisation.

• Result was negative and so the isolation was performed again.
• Elution of DNA was done but results were not satisfactory.
• Media (SOB and SOC) were prepared for competent cell preparation.
• Positive results were obtained for elution of DNA and protocol was optimised.
• Innoculated BL 21 in SOB.
• Presentation and questionnaire based on our project was prepared to educate the students in different colleges.
• Logo designing was initiated.

Week 3:

• Growth curve for Pseudomonas aeruginosa was done and results were analysed.
• Growth curve for BL 21 was done.
• Plasmid isolation of pET24a was performed.
• Competent cell of BL 21 was made and protocol was optimised.
• Seed stock for BL 21 competent cell was made.
• Transformation was performed.
• Primers required for our project were designed and ordered.
• For Human Practise questionnaire for hospitals were prepared.
• Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.
• Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance.
• Logo design was reviewed and changes were made.
• Approached various funding agencies.
• Collaboration with Uppsala was confirmed.

Week 4:

• BL21 was subcultured.
• Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.
• Chloramphenicol plates were prepared for pSB1C3 amplification.
• Methicillin Resistant Staphylococcus aureus were obtained.
• Final safety forms were filled.
• First step was taken for wiki page and its theme was discussed.
• Responses for various questionnaire was collected and a statistical analysis was carried out.
• Results were presented to the faculty members of our department.
• Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.

SEPTEMBER 2015

Week 1:

• gBlocks of ftsZ was obtained.
• PCR amplification of the ftsZ was done.
• Results were found to be negative.
• Hence they were troubleshooted.
• Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.

Week 2:

• gBlocks of thuricin was obtained.
• PCR amplification of the gene was done.
• This amplified gene was cloned into pSB1C3.
• Growth curve for Staphylococcus aureus was done.
• Human practices team presented in Madha Engineering College and SBOA.

Week 3:

• Got bactofencin gBlocks and PCR amplification was done.
• Then cloning was done in pSB1C3.
• Cloned Thuricin and Bactofencin were submitted as parts.
• Public interview regarding antibiotic resistance was taken.
• Descriptions were uploaded in wiki page.