Difference between revisions of "Team:DTU-Denmark/Journal"

 
Line 3: Line 3:
 
     <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 
     <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
−
   
 
−
   
 
−
    <link href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css" rel="stylesheet">
 
−
    <link href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css" rel="stylesheet">
 
−
    <link href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css" rel="stylesheet">
 
−
    <link href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css" rel="stylesheet">
 
−
    <link href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css" rel="stylesheet">
 
−
    <link href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css" rel="stylesheet">
 
  
−
   
+
      <link rel=stylesheet href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css">
 +
 
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
 +
 
     <!-- MathJax (LaTeX for the web) -->
 
     <!-- MathJax (LaTeX for the web) -->
 
     <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
 
     <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
−
    <!-- HTML5 shim and Respond.js for IE8 support of HTML5 elements and media queries -->
 
−
    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
 
−
    <!--[if lt IE 9]>
 
−
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
 
−
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
 
−
    <![endif]-->
 
 
      
 
      
 
   </head>
 
   </head>
Line 44: Line 37:
 
             <i class="fa fa-bars"></i>
 
             <i class="fa fa-bars"></i>
 
           </button>
 
           </button>
−
           <a class="navbar-brand navimg" href="#"><img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png"></a>
+
           <a class="navbar-brand navimg" href="#"><img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png"></a>
 
         </div>
 
         </div>
 
         <!-- Collect the nav links, forms, and other content for toggling -->
 
         <!-- Collect the nav links, forms, and other content for toggling -->
 
         <div class="collapse navbar-collapse" id="nav-collapse">
 
         <div class="collapse navbar-collapse" id="nav-collapse">
 
           <ul class="nav navbar-nav">
 
           <ul class="nav navbar-nav">
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark"
+
                <a href="/Team:DTU-Denmark"
−
  >Home
+
                  >Home
−
                                          </a></li>
+
                  </a></li>
−
<li  
+
                <li  
−
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
−
  >
+
                >
−
<a href=""
+
                <a href=""
−
  class="dropdown-toggle"
+
                  class="dropdown-toggle"
−
  data-toggle="dropdown"
+
                  data-toggle="dropdown"
−
  role="button"
+
                  role="button"
−
  aria-expanded="false">Team and Attributions
+
                  aria-expanded="false">Team and Attributions
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Team"
+
                <a href="/Team:DTU-Denmark/Team"
−
  >Team
+
                  >Team
−
                                          </a></li>
+
                  </a></li>
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Attributions"
+
                <a href="/Team:DTU-Denmark/Attributions"
−
  >Attributions
+
                  >Attributions
−
                                          </a></li></ul></li>
+
                  </a></li></ul></li>
−
<li  
+
                <li  
−
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
 +
                >
 +
                <a href=""
 +
                  class="dropdown-toggle"
 +
                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Project
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Overview"
 +
                  >Overview
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Background"
 +
                  >Background
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/MAGE"
 +
                  >MAGE subtilis
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Tyrocidine"
 +
                  >Tyrocidine
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Chip"
 +
                  >Lab-on-a-disc
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Inteins"
 +
                  >Inteins
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Detection"
 +
                  >Detection of NRP
 +
                  </a></li></ul></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Practices"
 +
                  >Human Practices
 +
                  </a></li>
 +
                <li
 +
               
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
−
  >
+
                >
−
<a href=""
+
                <a href=""
−
  class="dropdown-toggle"
+
                  class="dropdown-toggle"
−
  data-toggle="dropdown"
+
                  data-toggle="dropdown"
−
  role="button"
+
                  role="button"
−
  aria-expanded="false">Project
+
                  aria-expanded="false">Parts Collection
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Project/Overview"
+
                <a href="/Team:DTU-Denmark/Parts"
−
  >Overview
+
                  >Parts
−
                                          </a></li>
+
                  </a></li>
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Project/Background"
+
                <a href="/Team:DTU-Denmark/Description"
−
  >Background
+
                  >Characterisation of xylR
−
                                          </a></li>
+
                  </a></li></ul></li>
−
<li >
+
                <li class="active">
−
<a href="/Team:DTU-Denmark/Project/MAGE"
+
                <a href="/Team:DTU-Denmark/Journal"
−
  >MAGE Subtilis
+
                  >Journal
−
                                          </a></li>
+
                  </a></li>
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Project/Surfactin"
+
                <a href="/Team:DTU-Denmark/Software"
−
  >Surfactin
+
                  >Software
−
                                          </a></li>
+
                  </a></li>
−
<li >
+
                <li  
−
<a href="/Team:DTU-Denmark/Project/Tyrocidine"
+
               
−
  >Tyrocidine
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Project/Chip"
+
−
  >Lab-on-a-disc
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Project/Intein"
+
−
  >Inteins
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Project/Detection"
+
−
  >Detection of NRP
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Project/POC_MAGE"
+
−
  >Proof of concept of MAGE in B. subtilis
+
−
                                          </a></li></ul></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Practices"
+
−
  >Human Practices
+
−
                                          </a></li>
+
−
<li  
+
−
+
 
                      
 
                      
−
                 
+
               
 
                      
 
                      
−
                 
+
               
−
  >
+
−
<a href=""
+
−
  class="dropdown-toggle"
+
−
  data-toggle="dropdown"
+
−
  role="button"
+
−
  aria-expanded="false">Parts Collection
+
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Parts"
+
−
  >Parts
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Description"
+
−
  >Characterization of xylR
+
−
                                          </a></li></ul></li>
+
−
<li class="active">
+
−
<a href="/Team:DTU-Denmark/Journal"
+
−
  >Journal
+
−
                                          </a></li>
+
−
<li >
+
−
<a href="/Team:DTU-Denmark/Software"
+
−
  >Software
+
−
                                          </a></li>
+
−
<li
+
−
+
 
                      
 
                      
−
                 
+
               
−
                   
+
                >
−
                 
+
                <a href=""
−
                   
+
                  class="dropdown-toggle"
−
                 
+
                  data-toggle="dropdown"
−
  >
+
                  role="button"
−
<a href=""
+
                  aria-expanded="false">Achievements
−
  class="dropdown-toggle"
+
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
−
  data-toggle="dropdown"
+
                <li >
−
  role="button"
+
                <a href="/Team:DTU-Denmark/Achievements"
−
  aria-expanded="false">Achievements
+
                  >Key Achievements
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
                  </a></li>
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Achievements"
+
                <a href="/Team:DTU-Denmark/Collaborations"
−
  >Key Achievements
+
                  >Collaborations
−
                                          </a></li>
+
                  </a></li>
−
<li >
+
                <li >
−
<a href="/Team:DTU-Denmark/Collaborations"
+
                <a href="/Team:DTU-Denmark/Judging_Form"
−
  >Collaborations
+
                  >Judging Form
−
                                          </a></li>
+
                  </a></li></ul></li>
−
<li >
+
−
<a href="/Team:DTU-Denmark/Judging%20Form"
+
−
  >Judging Form
+
−
                                          </a></li></ul></li>
+
 
             <li class="hidden-xs">
 
             <li class="hidden-xs">
 
               <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
 
               <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
Line 211: Line 192:
 
             <li class="hidden-lg">
 
             <li class="hidden-lg">
 
               <a href="https://igem.org" target="_blank" class="navimg">
 
               <a href="https://igem.org" target="_blank" class="navimg">
−
                 <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
+
                 <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
 
               </a>
 
               </a>
 
             </li>
 
             </li>
Line 218: Line 199:
 
       </div>
 
       </div>
 
     </nav>
 
     </nav>
−
    <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
+
 
−
      <div class="container-fluid" id="scrollspy">
+
 
−
        <ul class="nav navbar-nav">
+
<nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
−
          <li><a class="page-scroll" href="#Lab-notebooks">Lab-notebooks</a></li><li><a class="page-scroll" href="#Protocols">Protocols</a></li><li><a class="page-scroll" href="#Lab-Notebook-">Lab Notebook </a></li><li><a class="page-scroll" href="#Timeline">Timeline</a></li><li><a class="page-scroll" href="#References">References</a></li>
+
  <div class="container-fluid" id="scrollspy">
−
        </ul>
+
    <ul class="nav navbar-nav">
−
      </div>
+
      <li><a class="page-scroll" href="#Protocols">Protocols</a></li><li><a class="page-scroll" href="#LabNotebook">Lab Notebook </a></li><li><a class="page-scroll" href="#Timeline">Timeline</a></li>
−
    </nav>
+
    </ul>
−
    <div class="jumbotron hero bg-hero">
+
  </div>
 +
</nav>
 +
 
 +
 
 +
<div class="jumbotron hero" style="background-image: url(https://static.igem.org/mediawiki/2015/0/0b/DTU-Denmark_hero.jpg)">
 
       <div class="igem-logo visible-lg">
 
       <div class="igem-logo visible-lg">
 
         <a href="https://igem.org" target="_blank">
 
         <a href="https://igem.org" target="_blank">
−
           <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
+
           <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
 
         </a>
 
         </a>
 
       </div>
 
       </div>
Line 235: Line 220:
 
           <div class="col-md-12">
 
           <div class="col-md-12">
 
              
 
              
−
            <h3><br></h3>
+
<h3><br></h3>
−
            <h1>Journal</h1>
+
<h1>Journal</h1>
−
           
+
 
 
             <hr/>
 
             <hr/>
 +
           
 +
           
 
             <ul class="list-inline hidden-xs hidden-sm">
 
             <ul class="list-inline hidden-xs hidden-sm">
−
             
 
−
              <li>
 
−
                <a id="Lab-notebooks-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Lab-notebooks">Lab-notebooks</a>
 
−
              </li>
 
 
                
 
                
 
               <li>
 
               <li>
Line 250: Line 233:
 
                
 
                
 
               <li>
 
               <li>
−
                 <a id="Lab-Notebook--submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Lab-Notebook-">Lab Notebook </a>
+
                 <a id="LabNotebook-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#LabNotebook">Lab Notebook </a>
 
               </li>
 
               </li>
 
                
 
                
 
               <li>
 
               <li>
 
                 <a id="Timeline-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Timeline">Timeline</a>
 
                 <a id="Timeline-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Timeline">Timeline</a>
−
              </li>
 
−
             
 
−
              <li>
 
−
                <a id="References-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#References">References</a>
 
 
               </li>
 
               </li>
 
                
 
                
 
             </ul>
 
             </ul>
 
             <ul class="list-inline visible-xs visible-sm">
 
             <ul class="list-inline visible-xs visible-sm">
−
             
 
−
              <li>
 
−
                <a id="Lab-notebooks-submenu" class="page-scroll" href="#Lab-notebooks">Lab-notebooks</a>
 
−
              </li>
 
 
                
 
                
 
               <li>
 
               <li>
Line 273: Line 248:
 
                
 
                
 
               <li>
 
               <li>
−
                 <a id="Lab-Notebook--submenu" class="page-scroll" href="#Lab-Notebook-">Lab Notebook </a>
+
                 <a id="LabNotebook-submenu" class="page-scroll" href="#LabNotebook">Lab Notebook </a>
 
               </li>
 
               </li>
 
                
 
                
 
               <li>
 
               <li>
 
                 <a id="Timeline-submenu" class="page-scroll" href="#Timeline">Timeline</a>
 
                 <a id="Timeline-submenu" class="page-scroll" href="#Timeline">Timeline</a>
−
              </li>
 
−
             
 
−
              <li>
 
−
                <a id="References-submenu" class="page-scroll" href="#References">References</a>
 
 
               </li>
 
               </li>
 
                
 
                
 
             </ul>
 
             </ul>
 +
           
 +
           
 
           </div>
 
           </div>
 
         </div>
 
         </div>
 +
       
 
         <div class="row">
 
         <div class="row">
 
           <div class="col-md-12">
 
           <div class="col-md-12">
 
             Scroll down for more<br>
 
             Scroll down for more<br>
−
             <a href="#Lab-notebooks" class="btn btn-circle btn-transparent page-scroll">
+
             <a href="#Protocols" class="btn btn-circle btn-transparent page-scroll">
 
               <i class="fa fa-angle-double-down"></i>
 
               <i class="fa fa-angle-double-down"></i>
 
             </a>
 
             </a>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
 +
       
 
       </div>
 
       </div>
 
     </div>
 
     </div>
 
      
 
      
−
+
      
−
<div id="Lab-notebooks">
+
       
−
  <div class="container">
+
−
     <div class="row col-md-12">
+
−
      <h1>
+
−
        Lab-notebooks
+
−
      </h1>
+
−
      <p><a href="/wiki/images/1/10/DTU-Denmark_labnote_MAGE_proof.pdf" target="_blank">MAGE competent cells</a></p>
+
−
 
+
−
<p><a href="/wiki/images/0/01/DTU-Denmark_Bsubtilis_strains_for_mage.pdf" target="_blank">MAGE proof of concept</a></p>
+
−
 
+
−
<p><a href="/wiki/images/c/cf/DTU-Denmark_Tyrocidine_labnotebook.pdf" target="_blank">Expression of tyrocidine</a></p>
+
−
 
+
−
<p>&nbsp;</p>
+
−
 
+
−
<p>&nbsp;</p>
+
−
 
+
−
    </div>
+
−
  </div>
+
−
</div>
+
−
+
−
+
 
<div id="Protocols">
 
<div id="Protocols">
 
   <div class="container">
 
   <div class="container">
 +
 
 
     <div class="row col-md-12">
 
     <div class="row col-md-12">
 
       <h1>
 
       <h1>
 
         Protocols
 
         Protocols
 
       </h1>
 
       </h1>
−
      <div aria-multiselectable="true" class="panel-group" id="accordion" role="tablist">
+
       
 +
        <div aria-multiselectable="true" class="panel-group" id="accordion" role="tablist">
 
<div class="panel panel-default">
 
<div class="panel panel-default">
 
<div class="panel-heading" id="headingOne" role="tab">
 
<div class="panel-heading" id="headingOne" role="tab">
Line 334: Line 291:
 
<div aria-labelledby="headingOne" class="panel-collapse collapse" id="collapseOne" role="tabpanel">
 
<div aria-labelledby="headingOne" class="panel-collapse collapse" id="collapseOne" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">Needed materials: 100 ml. TAE buffer. 1gr. agarose. ethidium bromide</div>
+
<div class="panel-body">Needed materials: 100 ml&nbsp;TAE buffer,&nbsp;1gr. agarose,&nbsp;ethidium bromide.</div>
  
−
<div class="panel-body">Mix 100 ml. TAE buffer with 1 gr. agarose powder, heat in microwave till the agarose is disolved, let it cool till it can be handled with bare hands, add 10 &micro;l&nbsp;ethidium bromide. pour into mold containing slots for wells, and let cool.</div>
+
<div class="panel-body">Mix 100 ml. TAE buffer with 1 gr. agarose powder, heat in microwave till the agarose is disolved, let it cool till it can be handled with bare hands, add 10 &micro;l&nbsp;ethidium bromide. Then&nbsp;pour the mixture&nbsp;into a mold containing slots for wells, and let cool.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 348: Line 305:
 
<div aria-labelledby="headingTwo" class="panel-collapse collapse" id="collapseTwo" role="tabpanel">
 
<div aria-labelledby="headingTwo" class="panel-collapse collapse" id="collapseTwo" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">Needed materials: 100 ml. TAE buffer. 1gr. agarose. ethidium bromide</div>
+
<div class="panel-body">Set gel into electrophoresis chamber filled with 1X TAE buffer so the wells are covered.&nbsp;<br />
−
 
+
Mix 5 &micro;l of&nbsp;your PCR reactions/sample with 1&nbsp;&micro;l 6X loading buffer.<br />
−
<p aria-controls="ui-id-36" aria-expanded="true" aria-selected="false" role="tab" tabindex="0">set gel into&nbsp;Electrophoresis chamber filled with 1X TAE buffer so the wells are covered.&nbsp;<br />
+
Pipette 5&nbsp;&micro;l into each well,&nbsp;pour 2-3&nbsp;&micro;l ladder in some so the size can be seen.<br />
−
mix 5 &micro;l of&nbsp;your PCR reactions/sample with 1&nbsp;&micro;l 6X loading buffer.<br />
+
Run at 140 V, for 30-40 min.</div>
−
pipette 5&nbsp;&micro;l into each well, pour 2-3&nbsp;&micro;l ladder in some so the size can be seen.<br />
+
−
run at 140 V, for 30-40 min</p>
+
 
</div>
 
</div>
 
</div>
 
</div>
Line 367: Line 322:
 
<div class="panel-body">Chemo competens is a way of getting E. coli to be able to take up new DNA, since this cant be done naturally like B. subtilis, then chemocompetent cells have to be made.</div>
 
<div class="panel-body">Chemo competens is a way of getting E. coli to be able to take up new DNA, since this cant be done naturally like B. subtilis, then chemocompetent cells have to be made.</div>
  
−
<div class="panel-body">Protocol can be found&nbsp;<a href="/wiki/images/c/cd/DTU-Denmark_Chemocompetentcells_protocol.pdf" target="_blank">here</a></div>
+
<div class="panel-body">Protocol can be found&nbsp;<a href="https://static.igem.org/mediawiki/2015/c/cd/DTU-Denmark_Chemocompetentcells_protocol.pdf" target="_blank">here</a></div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 379: Line 334:
 
<div aria-labelledby="headingFour" class="panel-collapse collapse" id="collapseFour" role="tabpanel">
 
<div aria-labelledby="headingFour" class="panel-collapse collapse" id="collapseFour" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">Needed materials: a 40% glycerol solution, bacteria of your choice, cryo tubes</div>
+
<div class="panel-body">Needed materials: a 40% glycerol solution, bacteria of your choice, cryo tubes.</div>
  
−
<div class="panel-body">mix glycerol and bacteria 50/50 in a cryo tube and place in a -80 frezzer till needed</div>
+
<div class="panel-body">Mix glycerol and bacteria 50/50 in a cryo tube and place in a -80 frezzer till needed.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 395: Line 350:
 
<p>A lot of lab work requires plates in order to produce results, and often you need a special concentration, a unique antibiotic mixed into the plates or a different composition than what is available at the lab you are in. If that is the case, the plate needs to be made from scratch, or somewhat along the way.</p>
 
<p>A lot of lab work requires plates in order to produce results, and often you need a special concentration, a unique antibiotic mixed into the plates or a different composition than what is available at the lab you are in. If that is the case, the plate needs to be made from scratch, or somewhat along the way.</p>
  
−
<p>Protocol can be found <a href="/wiki/images/e/e2/DTU-Denmark_making_plates_protocol.pdf" target="_blank">her</a>e</p>
+
<p>Protocol can be found <a href="https://static.igem.org/mediawiki/2015/e/e2/DTU-Denmark_making_plates_protocol.pdf" target="_blank">here</a>.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 407: Line 362:
 
<div aria-labelledby="headingSix" class="panel-collapse collapse" id="collapseSix" role="tabpanel">
 
<div aria-labelledby="headingSix" class="panel-collapse collapse" id="collapseSix" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">For our experiments we have used minimal media designed for B. subtilis. The plates have been made by following this protocol (for 1 liter)</div>
+
<div class="panel-body">For the&nbsp;experiments a minimal media designed for <em>B. subtilis</em> has been used. The plates have been made by following this protocol (for 1 liter).</div>
  
−
<div class="panel-body">Protocol can be found <a href="/wiki/images/5/52/DTU-Denmark_Minimal_media_protocol.pdf" target="_blank">here</a></div>
+
<div class="panel-body">Protocol can be found <a href="https://static.igem.org/mediawiki/2015/5/52/DTU-Denmark_Minimal_media_protocol.pdf" target="_blank">here</a>.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 421: Line 376:
 
<div aria-labelledby="headingSeven" class="panel-collapse collapse" id="collapseSeven" role="tabpanel">
 
<div aria-labelledby="headingSeven" class="panel-collapse collapse" id="collapseSeven" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">in order to work with plasmids, then they needs to be purified, and condensed, this is done bu minipreping the bacteria, and afterwards the concentration can be investigating using&nbsp;NanoDrop&trade;.</div>
+
<div class="panel-body">In order to work with plasmids, then they needs to be purified&nbsp;and condensed, this is done bu minipreping the bacteria, and afterwards the concentration can be investigating using&nbsp;NanoDrop&trade;.</div>
  
−
<div class="panel-body">Protocol <a href="/wiki/images/4/48/DTU-Denmark_Miniprep_protocol.pdf" target="_blank">here</a>.</div>
+
<div class="panel-body">Protocol can be found <a href="https://static.igem.org/mediawiki/2015/4/48/DTU-Denmark_Miniprep_protocol.pdf" target="_blank">here</a>.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 435: Line 390:
 
<div aria-labelledby="headingEigth" class="panel-collapse collapse" id="collapseEigth" role="tabpanel">
 
<div aria-labelledby="headingEigth" class="panel-collapse collapse" id="collapseEigth" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">Bacillus can be made Competent which makes it easy to alter the genome, so wanted mutaions can be inserted, this is a unique way of investigateing new pathways.</div>
+
<div class="panel-body">Bacillus can be made competent allowing an&nbsp;easy alteration of&nbsp;the genome and&nbsp;so wanted mutaions can be inserted, this is a unique way of investigateing new pathways.</div>
  
−
<div class="panel-body">Protocol can be found&nbsp;<a href="/wiki/images/2/29/DTU-Denmark_natural_competent_subtilis_protocol.pdf" target="_blank">here</a></div>
+
<div class="panel-body">Protocol can be found&nbsp;<a href="https://static.igem.org/mediawiki/2015/2/29/DTU-Denmark_natural_competent_subtilis_protocol.pdf" target="_blank">here</a>.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 451: Line 406:
 
<div class="panel-body">Electrotoration of&nbsp;bacteria is used in order to introduce mutations in the lagging strand of the strain, this technique&nbsp;are used in order to incorporate wanted mutations in a strain of&nbsp;Bacillus subtilis 168.</div>
 
<div class="panel-body">Electrotoration of&nbsp;bacteria is used in order to introduce mutations in the lagging strand of the strain, this technique&nbsp;are used in order to incorporate wanted mutations in a strain of&nbsp;Bacillus subtilis 168.</div>
  
−
<div class="panel-body">Protocol can be found&nbsp;<a href="/wiki/images/0/0e/DTU-Denmark_MAGE_in_baccilus.pdf" target="_blank">here</a></div>
+
<div class="panel-body">Protocol can be found&nbsp;<a href="https://static.igem.org/mediawiki/2015/0/0e/DTU-Denmark_MAGE_in_baccilus.pdf" target="_blank">here</a>.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 458: Line 413:
 
<div class="panel panel-default">
 
<div class="panel panel-default">
 
<div class="panel-heading" id="headingTen" role="tab">
 
<div class="panel-heading" id="headingTen" role="tab">
−
<h4 class="panel-title"><a aria-controls="collapseTen" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTen" role="button">Preperation of Electro Competent &nbsp;Bacillus subtilis 168</a></h4>
+
<h4 class="panel-title"><a aria-controls="collapseTen" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTen" role="button">Preperation of Electro Competent&nbsp;Bacillus subtilis 168</a></h4>
 
</div>
 
</div>
  
Line 465: Line 420:
 
<div class="panel-body">Electro competent cells are used in our MAGE part of the project, so a batch of cells was prepared using the following protocol.</div>
 
<div class="panel-body">Electro competent cells are used in our MAGE part of the project, so a batch of cells was prepared using the following protocol.</div>
  
−
<div class="panel-body">Protocol can be found&nbsp;<a href="/wiki/images/2/2e/DTU-Denmark_Electro_competent_protocol.pdf" target="_blank">here</a></div>
+
<div class="panel-body">Protocol can be found&nbsp;<a href="https://static.igem.org/mediawiki/2015/2/2e/DTU-Denmark_Electro_competent_protocol.pdf" target="_blank">here</a></div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 471: Line 426:
 
</div>
 
</div>
  
 +
       
 
     </div>
 
     </div>
 +
   
 
   </div>
 
   </div>
 
</div>
 
</div>
−
+
   
−
+
       
−
<div id="Lab-Notebook-">
+
<div id="LabNotebook">
 
   <div class="container">
 
   <div class="container">
 +
 
 
     <div class="row col-md-12">
 
     <div class="row col-md-12">
 
       <h1>
 
       <h1>
 
         Lab Notebook  
 
         Lab Notebook  
 
       </h1>
 
       </h1>
−
      <div class="panel panel-default">
+
       
 +
        <div class="panel panel-default">
 
<div class="panel-heading" id="headingTwentyone" role="tab">
 
<div class="panel-heading" id="headingTwentyone" role="tab">
 
<h4 class="panel-title"><a aria-controls="collapseTwentyone" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTwentyone" role="button">Expression of Tyrocidine</a></h4>
 
<h4 class="panel-title"><a aria-controls="collapseTwentyone" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTwentyone" role="button">Expression of Tyrocidine</a></h4>
Line 489: Line 448:
 
<div aria-labelledby="headingTwentytwo" class="panel-collapse collapse" id="collapseTwentyone" role="tabpanel">
 
<div aria-labelledby="headingTwentytwo" class="panel-collapse collapse" id="collapseTwentyone" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">The lab book for expression of Tyrocidine&nbsp;can be found <a href="/wiki/images/c/cf/DTU-Denmark_Tyrocidine_labnotebook.pdf" onclick="window.open(this.href, '', 'resizable=no,status=no,location=no,toolbar=no,menubar=no,fullscreen=no,scrollbars=no,dependent=no'); return false;">here</a>.&nbsp;</div>
+
<div class="panel-body">The lab book for expression of tyrocidine&nbsp;can be found <a href="https://static.igem.org/mediawiki/2015/c/cf/DTU-Denmark_Tyrocidine_labnotebook.pdf" onclick="window.open(this.href, '', 'resizable=no,status=no,location=no,toolbar=no,menubar=no,fullscreen=no,scrollbars=no,dependent=no'); return false;">here</a>.&nbsp;</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 501: Line 460:
 
<div aria-labelledby="headingTwentytwo" class="panel-collapse collapse" id="collapseTwentytwo" role="tabpanel">
 
<div aria-labelledby="headingTwentytwo" class="panel-collapse collapse" id="collapseTwentytwo" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">The lab book for the generation of oligo-competent strains can be found <a href="/wiki/images/7/75/DTU-Denmark_straingeneration.pdf">here</a>.</div>
+
<div class="panel-body">The lab book for the generation of oligo-competent strains can be found <a href="https://static.igem.org/mediawiki/2015/7/75/DTU-Denmark_straingeneration.pdf">here</a>.</div>
 
</div>
 
</div>
 
</div>
 
</div>
Line 513: Line 472:
 
<div aria-labelledby="headingTwentythree" class="panel-collapse collapse" id="collapseTwentythree" role="tabpanel">
 
<div aria-labelledby="headingTwentythree" class="panel-collapse collapse" id="collapseTwentythree" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">To approve a lab-on-a-disc concept as a screening device, the UV-vis abasobancee were measured&nbsp;<a href="/wiki/images/6/62/DTU-Denmark_bloodcell_lysis.pdf">here</a>.&nbsp;</div>
+
<div class="panel-body">To approve a lab-on-a-disc concept as a screening device, the UV-vis abasobancee were measured&nbsp;<a href="https://static.igem.org/mediawiki/2015/6/62/DTU-Denmark_bloodcell_lysis.pdf">here</a>.&nbsp;</div>
−
 
+
−
<div class="panel-body">&nbsp;</div>
+
−
 
+
−
<div class="panel-body">&nbsp;</div>
+
 
</div>
 
</div>
 
</div>
 
</div>
Line 523: Line 478:
  
 
<div class="panel panel-default">
 
<div class="panel panel-default">
−
<div class="panel-heading" id="headingTwentythree" role="tab">
+
<div class="panel-heading" id="headingTwentyfour" role="tab">
−
<h4 class="panel-title"><a aria-controls="collapseTwentythree" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTwentythree" role="button">MAGE&nbsp;proof of concept</a></h4>
+
<h4 class="panel-title"><a aria-controls="collapseTwentyfour" aria-expanded="false" class="collapsed" data-parent="#accordion" data-toggle="collapse" href="#collapseTwentyfour" role="button">MAGE&nbsp;proof of concept</a></h4>
 
</div>
 
</div>
  
−
<div aria-labelledby="headingTwentythree" class="panel-collapse collapse" id="collapseTwentythree" role="tabpanel">
+
<div aria-labelledby="headingTwentyfour" class="panel-collapse collapse" id="collapseTwentyfour" role="tabpanel">
 
<div class="panel-body">
 
<div class="panel-body">
−
<div class="panel-body">See our labnotebook <a href="/wiki/images/1/10/DTU-Denmark_labnote_MAGE_proof.pdf">here.</a></div>
+
<div class="panel-body">See our labnotebook <a href="https://static.igem.org/mediawiki/2015/1/10/DTU-Denmark_labnote_MAGE_proof.pdf">here.</a></div>
−
 
+
−
<div class="panel-body">&nbsp;</div>
+
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
  
 +
       
 
     </div>
 
     </div>
 +
   
 
   </div>
 
   </div>
 
</div>
 
</div>
−
+
   
−
+
       
 
<div id="Timeline">
 
<div id="Timeline">
 
   <div class="container">
 
   <div class="container">
−
     <div class="row">
+
 
−
      <div class="col-lg-12">
+
 
−
        <h2>Timeline</h2>
+
     <div class="row col-md-12">
 +
      <h1>
 +
        Timeline
 +
      </h1>
 +
       
 +
       
 
          
 
          
−
      </div>
 
 
     </div>
 
     </div>
 +
   
 
     <ul class="timeline">
 
     <ul class="timeline">
 
      
 
      
Line 559: Line 519:
 
           </div>
 
           </div>
 
           <div class="timeline-body">
 
           <div class="timeline-body">
−
             <p style="text-align: justify;"><img alt="" src="/wiki/images/c/c5/DTU-Denmark_timeline_novoamb.png" style="height: 158px; width: 250px; float: left;" />Our team member Pernille spent an afternoon giving a presentation on cell factories and synthetic biology to 30 highschool students. She says: &quot;Synthetic biology is all about being creative and use your imagination&nbsp;to design bio-solutions that can impact the world. For that exact reason, it is neccesary to discuss which ethical values tat should drive research&quot;. The highschool students had lots of questions and opinion for the discussion about ethics.</p>
+
             <p style="text-align: justify;"><img alt="" src="https://static.igem.org/mediawiki/2015/c/c5/DTU-Denmark_timeline_novoamb.png" style="height: 158px; width: 250px; float: left;" />Our team member Pernille spent an afternoon giving a presentation on cell factories and synthetic biology to 30 highschool students. She says: &quot;Synthetic biology is all about being creative and use your imagination&nbsp;to design bio-solutions that can impact the world. For that exact reason, it is neccesary to discuss which ethical values tat should drive research&quot;. The highschool students had lots of questions and opinion for the discussion about ethics.</p>
  
 
<p style="text-align: justify;">&nbsp;</p>
 
<p style="text-align: justify;">&nbsp;</p>
Line 577: Line 537:
 
             <p>We are happy to announce that the VWR&nbsp;is contributing our team with&nbsp;their awesome products!</p>
 
             <p>We are happy to announce that the VWR&nbsp;is contributing our team with&nbsp;their awesome products!</p>
  
−
<p><img alt="" src="/wiki/images/3/37/DTU-Denmark_VWR_lab.jpg" style="width: 400px; height: 533px;" /></p>
+
<p><img alt="" src="https://static.igem.org/mediawiki/2015/3/37/DTU-Denmark_VWR_lab.jpg" style="width: 400px; height: 533px;" /></p>
  
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
Line 595: Line 555:
 
             <p>Today we had a visit by&nbsp;Susanne Basse from&nbsp;Fisher Scientific. She&nbsp;came by our office with a wagon full of items, Thanks!&nbsp;</p>
 
             <p>Today we had a visit by&nbsp;Susanne Basse from&nbsp;Fisher Scientific. She&nbsp;came by our office with a wagon full of items, Thanks!&nbsp;</p>
  
−
<p><img alt="" src="/wiki/images/c/c7/DTU-Denmark_Fisher_Scientific_visit.png" style="width: 300px; height: 280px;" /></p>
+
<p><img alt="" src="/files/Fisher%20Scientific%20visit.png" style="width: 300px; height: 280px;" /></p>
  
 
           </div>
 
           </div>
Line 611: Line 571:
 
             <p>We are pleased&nbsp;to announce that Macrogen is sponsoring our team with sequencing!</p>
 
             <p>We are pleased&nbsp;to announce that Macrogen is sponsoring our team with sequencing!</p>
  
−
<p><a href="http://www.macrogen.com/" target="_blank"><img alt="" src="/wiki/images/1/11/DTU-Denmark_Marcogen.jpg" style="width: 300px; height: 199px;" /></a></p>
+
<p><a href="http://www.macrogen.com/" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/1/11/DTU-Denmark_Marcogen.jpg" style="width: 300px; height: 199px;" /></a></p>
  
 
           </div>
 
           </div>
Line 627: Line 587:
 
             <p>We are happy to announce that AKG Acoustics is sponsoring our team with headphones for presentations in Boston!&nbsp;</p>
 
             <p>We are happy to announce that AKG Acoustics is sponsoring our team with headphones for presentations in Boston!&nbsp;</p>
  
−
<p><a href="http://us.akg.com/akg-homepage-us.html" target="_blank"><img alt="" src="/wiki/images/9/9d/DTU-Denmark_AKG.png" style="width: 400px; height: 121px;" /></a></p>
+
<p><a href="http://us.akg.com/akg-homepage-us.html" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/9/9d/DTU-Denmark_AKG.png" style="width: 400px; height: 121px;" /></a></p>
  
 
           </div>
 
           </div>
Line 637: Line 597:
 
         <div class="timeline-panel">
 
         <div class="timeline-panel">
 
           <div class="timeline-heading">
 
           <div class="timeline-heading">
−
             <h4 class="timeline-title">Contributions from VWR - Bie & Berntsen A/S</h4>
+
             <h4 class="timeline-title">Contributions from VWR - Bie &amp; Berntsen A/S</h4>
 
             <p><small class="text-muted"><i class="glyphicon glyphicon-time"></i> 2015-08-11</small></p>
 
             <p><small class="text-muted"><i class="glyphicon glyphicon-time"></i> 2015-08-11</small></p>
 
           </div>
 
           </div>
Line 643: Line 603:
 
             <p>We are happy to announce that VWR - Bie &amp; Berntsen A/S is sponsoring our team with lab consumeables!</p>
 
             <p>We are happy to announce that VWR - Bie &amp; Berntsen A/S is sponsoring our team with lab consumeables!</p>
  
−
<p><a href="dk.vwr.com" target="_blank"><img alt="" src="/wiki/images/e/ea/DTU-Denmark_VWR.jpg" style="width: 400px; height: 76px;" /></a></p>
+
<p><a href="dk.vwr.com" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/e/ea/DTU-Denmark_VWR.jpg" style="width: 400px; height: 76px;" /></a></p>
  
 
           </div>
 
           </div>
Line 659: Line 619:
 
             <p>We are happy to announce that Fisher Scientific&nbsp;is sponsoring our team with lab materials!&nbsp;</p>
 
             <p>We are happy to announce that Fisher Scientific&nbsp;is sponsoring our team with lab materials!&nbsp;</p>
  
−
<p><a href="https://dk.fishersci.com/dk/" target="_blank"><img alt="" src="/wiki/images/b/b4/DTU-Denmark_Fisher_Scientific.jpg" style="width: 400px; height: 123px;" /></a></p>
+
<p><a href="https://dk.fishersci.com/dk/" target="_blank"><img alt="" src="/files/Fisher%20Scientific.jpg" style="width: 400px; height: 123px;" /></a></p>
  
 
           </div>
 
           </div>
Line 675: Line 635:
 
             <p>Today we took a day off from lab to go canoeing, followed by a barbeque at Chris&#39; place.</p>
 
             <p>Today we took a day off from lab to go canoeing, followed by a barbeque at Chris&#39; place.</p>
  
−
<p><img alt="" src="/wiki/images/6/6a/DTU-Denmark_Teambuilding.jpg" /></p>
+
<p><img alt="" src="https://static.igem.org/mediawiki/2015/6/6a/DTU-Denmark_Teambuilding.jpg" /></p>
  
 
           </div>
 
           </div>
Line 691: Line 651:
 
             <p>We are happy to announce that the SnapGene&nbsp;is contributing&nbsp;our team with lisences to their awesome product!</p>
 
             <p>We are happy to announce that the SnapGene&nbsp;is contributing&nbsp;our team with lisences to their awesome product!</p>
  
−
<p><a href="http://www.snapgene.com/" target="_blank"><img alt="" src="/wiki/images/5/58/DTU-Denmark_Snapgene.png" style="width: 400px; height: 113px;" /></a></p>
+
<p><a href="http://www.snapgene.com/" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/5/58/DTU-Denmark_Snapgene.png" style="width: 400px; height: 113px;" /></a></p>
  
 
           </div>
 
           </div>
Line 735: Line 695:
 
             <p>3 high school students;&nbsp;Simran, Noor, and Charlotte, joined us and Biotech Academy for 5 days to make biosensors that changes color, when water is poluted. Read more at&nbsp;the <a href="/Team:DTU-Denmark/Practices" target="_blank">Human Practice</a>&nbsp;and <a href="http://biotechacademy.dk">http://biotechacademy.dk</a>.</p>
 
             <p>3 high school students;&nbsp;Simran, Noor, and Charlotte, joined us and Biotech Academy for 5 days to make biosensors that changes color, when water is poluted. Read more at&nbsp;the <a href="/Team:DTU-Denmark/Practices" target="_blank">Human Practice</a>&nbsp;and <a href="http://biotechacademy.dk">http://biotechacademy.dk</a>.</p>
  
−
<p style="text-align: center;"><img alt="" src="/wiki/images/b/b8/DTU-Denmark_highschool_biotechacademy.jpg" style="width: 350px; height: 197px;" /></p>
+
<p style="text-align: center;"><img alt="" src="https://static.igem.org/mediawiki/2015/b/b8/DTU-Denmark_highschool_biotechacademy.jpg" style="width: 350px; height: 197px;" /></p>
  
 
           </div>
 
           </div>
Line 779: Line 739:
 
             <p>We are happy to announce that the Lundbeck Foundation is contributing&nbsp;financially to our team!</p>
 
             <p>We are happy to announce that the Lundbeck Foundation is contributing&nbsp;financially to our team!</p>
  
−
<p><a href="http://www.lundbeckfoundation.com/" target="_blank"><img alt="" src="/wiki/images/e/e2/DTU-Denmark_Lundbeckfonden.jpg" style="width: 348px; height: 96px;" /></a></p>
+
<p><a href="http://www.lundbeckfoundation.com/" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/e/e2/DTU-Denmark_Lundbeckfonden.jpg" style="width: 348px; height: 96px;" /></a></p>
  
 
           </div>
 
           </div>
Line 795: Line 755:
 
             <p>We are happy to announce that In Vitro A/S is sponsoring our team with lab consumeables!&nbsp;</p>
 
             <p>We are happy to announce that In Vitro A/S is sponsoring our team with lab consumeables!&nbsp;</p>
  
−
<p><a href="in-vitro.dk" target="_top"><img alt="" src="/wiki/images/7/7c/DTU-Denmark_In_Vitro.jpg" style="width: 400px; height: 156px;" /></a></p>
+
<p><a href="in-vitro.dk" target="_top"><img alt="" src="/files/In%20Vitro.jpg" style="width: 400px; height: 156px;" /></a></p>
  
 
           </div>
 
           </div>
Line 811: Line 771:
 
             <p>We are happy to announce that Frisnette is sponsoring our team with lab consumeables!&nbsp;</p>
 
             <p>We are happy to announce that Frisnette is sponsoring our team with lab consumeables!&nbsp;</p>
  
−
<p><a href="http://frisenette.dk/" target="_blank"><img alt="" src="/wiki/images/c/c8/DTU-Denmark_Frisenette.jpg" style="width: 330px; height: 85px;" /></a></p>
+
<p><a href="http://frisenette.dk/" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/c/c8/DTU-Denmark_Frisenette.jpg" style="width: 330px; height: 85px;" /></a></p>
  
 
           </div>
 
           </div>
Line 825: Line 785:
 
           </div>
 
           </div>
 
           <div class="timeline-body">
 
           <div class="timeline-body">
−
             <div class="pull-left"><img alt="" src="/wiki/images/e/e1/DTU-Denmark_timeline_W168.jpg" style="width: 120px; height: 160px;" /></div>
+
             <div class="pull-left"><img alt="" src="https://static.igem.org/mediawiki/2015/e/e1/DTU-Denmark_timeline_W168.jpg" style="width: 120px; height: 160px;" /></div>
  
 
<div class="pull-left">
 
<div class="pull-left">
Line 844: Line 804:
 
           </div>
 
           </div>
 
           <div class="timeline-body">
 
           <div class="timeline-body">
−
             <p style="text-align: center;"><img alt="" src="/wiki/images/f/fe/DTU-Denmark_timeline_biobrickworkshop.jpg" style="width: 350px; height: 198px;" /></p>
+
             <p style="text-align: center;"><img alt="" src="https://static.igem.org/mediawiki/2015/f/fe/DTU-Denmark_timeline_biobrickworkshop.jpg" style="width: 350px; height: 198px;" /></p>
  
 
<p>Southern University of&nbsp;Denmark and University of Copenhagen joined us for a three-day BioBrick tutorial, where we spent three days in the laboratory cloning, learning about iGEM, wiki design, and getting to know each other through social activities.</p>
 
<p>Southern University of&nbsp;Denmark and University of Copenhagen joined us for a three-day BioBrick tutorial, where we spent three days in the laboratory cloning, learning about iGEM, wiki design, and getting to know each other through social activities.</p>
Line 862: Line 822:
 
             <p>We are happy to announce that the Otto M&oslash;nsted Fonden&nbsp;is contributing&nbsp;financially to our team!</p>
 
             <p>We are happy to announce that the Otto M&oslash;nsted Fonden&nbsp;is contributing&nbsp;financially to our team!</p>
  
−
<p><a href="http://www.ottomoensted.dk/" target="_blank"><img alt="" src="/wiki/images/3/35/DTU-Denmark_Ottomoensted.png" style="width: 400px; height: 85px;" /></a></p>
+
<p><a href="http://www.ottomoensted.dk/" target="_blank"><img alt="" src="https://static.igem.org/mediawiki/2015/3/35/DTU-Denmark_Ottomoensted.png" style="width: 400px; height: 85px;" /></a></p>
  
 
           </div>
 
           </div>
Line 897: Line 857:
 
      
 
      
 
     </ul>
 
     </ul>
 +
 
   </div>
 
   </div>
 
</div>
 
</div>
−
+
      
−
+
      
−
<div id="References">
+
<footer class="container">
−
  <div class="container">
+
−
     <div class="row col-md-12">
+
−
      <h1>
+
−
        References
+
−
      </h1>
+
−
      <ol>
+
−
       
+
−
      </ol>
+
−
     </div>
+
−
  </div>
+
−
</div>
+
−
+
−
    <div class="container">
+
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-md-2 col-md-offset-2">
 
         <div class="col-md-2 col-md-offset-2">
 
           <a href="http://www.dtu.dk" target="_blank">
 
           <a href="http://www.dtu.dk" target="_blank">
−
             <img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png" height="130px">
+
             <img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png" height="130px">
 
           </a>
 
           </a>
 
         </div>
 
         </div>
Line 934: Line 882:
 
         <div class="col-md-2">
 
         <div class="col-md-2">
 
           <a href="https://igem.org" style="float:left" target="_blank">
 
           <a href="https://igem.org" style="float:left" target="_blank">
−
               <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png" height="90px">
+
               <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png" height="90px">
 
           </a>
 
           </a>
 
         </div>
 
         </div>
 
       </div>
 
       </div>
−
     </div>
+
     </footer>
 
     <div class="sponsors">
 
     <div class="sponsors">
−
       <div class="container-fluid">
+
       <div class="container">
 
         <div class="row">
 
         <div class="row">
 
            
 
            
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.lundbeckfonden.com/" target="_blank">
 
             <a href="http://www.lundbeckfonden.com/" target="_blank">
−
               <img src="/wiki/images/e/e2/DTU-Denmark_Lundbeckfonden.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/e/e2/DTU-Denmark_Lundbeckfonden.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 951: Line 899:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.ottomoensted.dk" target="_blank">
 
             <a href="http://www.ottomoensted.dk" target="_blank">
−
               <img src="/wiki/images/3/35/DTU-Denmark_Ottomoensted.png">
+
               <img src="https://static.igem.org/mediawiki/2015/3/35/DTU-Denmark_Ottomoensted.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 957: Line 905:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.novonordiskfonden.dk/en" target="_blank">
 
             <a href="http://www.novonordiskfonden.dk/en" target="_blank">
−
               <img src="/wiki/images/5/5c/DTU-Denmark_NovoNordiskFonden_logo.png">
+
               <img src="https://static.igem.org/mediawiki/2015/5/5c/DTU-Denmark_NovoNordiskFonden_logo.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 963: Line 911:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://dk.vwr.com/" target="_blank">
 
             <a href="https://dk.vwr.com/" target="_blank">
−
               <img src="/wiki/images/e/ea/DTU-Denmark_VWR.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/e/ea/DTU-Denmark_VWR.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 969: Line 917:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://frisenette.dk" target="_blank">
 
             <a href="http://frisenette.dk" target="_blank">
−
               <img src="/wiki/images/c/c8/DTU-Denmark_Frisenette.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/c/c8/DTU-Denmark_Frisenette.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 975: Line 923:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.in-vitro.dk/" target="_blank">
 
             <a href="http://www.in-vitro.dk/" target="_blank">
−
               <img src="/wiki/images/7/7c/DTU-Denmark_In_Vitro.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/7/7c/DTU-Denmark_In_Vitro.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 981: Line 929:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://dk.fishersci.com/dk/" target="_blank">
 
             <a href="https://dk.fishersci.com/dk/" target="_blank">
−
               <img src="/wiki/images/b/b4/DTU-Denmark_Fisher_Scientific.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/b/b4/DTU-Denmark_Fisher_Scientific.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 987: Line 935:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://us.akg.com/akg-homepage-us.html" target="_blank">
 
             <a href="http://us.akg.com/akg-homepage-us.html" target="_blank">
−
               <img src="/wiki/images/9/9d/DTU-Denmark_AKG.png">
+
               <img src="https://static.igem.org/mediawiki/2015/9/9d/DTU-Denmark_AKG.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 993: Line 941:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.macrogen.com/" target="_blank">
 
             <a href="http://www.macrogen.com/" target="_blank">
−
               <img src="/wiki/images/1/11/DTU-Denmark_Marcogen.jpg">
+
               <img src="https://static.igem.org/mediawiki/2015/1/11/DTU-Denmark_Marcogen.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 999: Line 947:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.snapgene.com/" target="_blank">
 
             <a href="http://www.snapgene.com/" target="_blank">
−
               <img src="/wiki/images/5/58/DTU-Denmark_Snapgene.png">
+
               <img src="https://static.igem.org/mediawiki/2015/5/58/DTU-Denmark_Snapgene.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 1,005: Line 953:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://www.neb.com/" target="_blank">
 
             <a href="https://www.neb.com/" target="_blank">
−
               <img src="/wiki/images/e/e0/DTU-Denmark_NEB_logo.png">
+
               <img src="https://static.igem.org/mediawiki/2015/e/e0/DTU-Denmark_NEB_logo.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>

Latest revision as of 15:12, 9 November 2015


Journal

Scroll down for more

Protocols

Needed materials: 100 ml TAE buffer, 1gr. agarose, ethidium bromide.
Mix 100 ml. TAE buffer with 1 gr. agarose powder, heat in microwave till the agarose is disolved, let it cool till it can be handled with bare hands, add 10 µl ethidium bromide. Then pour the mixture into a mold containing slots for wells, and let cool.
Set gel into electrophoresis chamber filled with 1X TAE buffer so the wells are covered. 
Mix 5 µl of your PCR reactions/sample with 1 µl 6X loading buffer.
Pipette 5 µl into each well, pour 2-3 µl ladder in some so the size can be seen.
Run at 140 V, for 30-40 min.
Chemo competens is a way of getting E. coli to be able to take up new DNA, since this cant be done naturally like B. subtilis, then chemocompetent cells have to be made.
Protocol can be found here
Needed materials: a 40% glycerol solution, bacteria of your choice, cryo tubes.
Mix glycerol and bacteria 50/50 in a cryo tube and place in a -80 frezzer till needed.

A lot of lab work requires plates in order to produce results, and often you need a special concentration, a unique antibiotic mixed into the plates or a different composition than what is available at the lab you are in. If that is the case, the plate needs to be made from scratch, or somewhat along the way.

Protocol can be found here.

For the experiments a minimal media designed for B. subtilis has been used. The plates have been made by following this protocol (for 1 liter).
Protocol can be found here.
In order to work with plasmids, then they needs to be purified and condensed, this is done bu minipreping the bacteria, and afterwards the concentration can be investigating using NanoDrop™.
Protocol can be found here.
Bacillus can be made competent allowing an easy alteration of the genome and so wanted mutaions can be inserted, this is a unique way of investigateing new pathways.
Protocol can be found here.
Electrotoration of bacteria is used in order to introduce mutations in the lagging strand of the strain, this technique are used in order to incorporate wanted mutations in a strain of Bacillus subtilis 168.
Protocol can be found here.
Electro competent cells are used in our MAGE part of the project, so a batch of cells was prepared using the following protocol.
Protocol can be found here

Lab Notebook

The lab book for expression of tyrocidine can be found here. 
The lab book for the generation of oligo-competent strains can be found here.
To approve a lab-on-a-disc concept as a screening device, the UV-vis abasobancee were measured here. 
See our labnotebook here.

Timeline

  • Biology of the Future

    2015-09-14

    Our team member Pernille spent an afternoon giving a presentation on cell factories and synthetic biology to 30 highschool students. She says: "Synthetic biology is all about being creative and use your imagination to design bio-solutions that can impact the world. For that exact reason, it is neccesary to discuss which ethical values tat should drive research". The highschool students had lots of questions and opinion for the discussion about ethics.

     

  • VWR to the rescue!

    2015-09-01

    We are happy to announce that the VWR is contributing our team with their awesome products!

     

  • Visit from Fisher Scientific!

    2015-08-26

    Today we had a visit by Susanne Basse from Fisher Scientific. She came by our office with a wagon full of items, Thanks! 

  • Contribution from Macrogen!

    2015-08-20

    We are pleased to announce that Macrogen is sponsoring our team with sequencing!

  • Contributions from AKG!

    2015-08-19

    We are happy to announce that AKG Acoustics is sponsoring our team with headphones for presentations in Boston! 

  • Contributions from VWR - Bie & Berntsen A/S

    2015-08-11

    We are happy to announce that VWR - Bie & Berntsen A/S is sponsoring our team with lab consumeables!

  • Contributions from Fisher Scientific

    2015-08-11

    We are happy to announce that Fisher Scientific is sponsoring our team with lab materials! 

  • Canoeing

    2015-08-07

    Today we took a day off from lab to go canoeing, followed by a barbeque at Chris' place.

  • Contribution from SnapGene

    2015-08-03

    We are happy to announce that the SnapGene is contributing our team with lisences to their awesome product!

  • Wiki Wizard

    2015-07-30

    We have uploaded the first beta version of the Wiki Wizard to GitHub

  • First BioBrick cloned into B. subtilis

    2015-07-30

    An expression cassette with lambda beta recombinase was sucesfully transformed into Bacillus subtilis. First step towards establishing MAGE in Bacillus subtilis completed!

  • BioBrick High School Project

    2015-07-23

    3 high school students; Simran, Noor, and Charlotte, joined us and Biotech Academy for 5 days to make biosensors that changes color, when water is poluted. Read more at the Human Practice and http://biotechacademy.dk.

  • First sequencing results

    2015-07-22

    We received our first sequencing results. One step closer to our first BioBrick!

  • Brevibacillus parabrevis arrives

    2015-07-16

    Brevibacillus parabrevis produces a lot of different antibiotics including tyrocidine. We will transfer the tyrocidine operon to our chassis Bacillus subtilis.

  • Contribution from the Lundbeck Foundation!

    2015-07-15

    We are happy to announce that the Lundbeck Foundation is contributing financially to our team!

  • Contributions from In Vitro!

    2015-05-06

    We are happy to announce that In Vitro A/S is sponsoring our team with lab consumeables! 

  • Contributions from Frisnette

    2015-04-30

    We are happy to announce that Frisnette is sponsoring our team with lab consumeables! 

  • We get our strain

    2015-04-28

    We get a plate of our chassis strain Bacillus subtilis W168.
    We can now begin the work in the laboratory.

  • BioBrick Workshop

    2015-04-24

    Southern University of Denmark and University of Copenhagen joined us for a three-day BioBrick tutorial, where we spent three days in the laboratory cloning, learning about iGEM, wiki design, and getting to know each other through social activities.

  • Contribution from the Otto Mønsted Fonden!

    2015-03-08

    We are happy to announce that the Otto Mønsted Fonden is contributing financially to our team!

  • First official team meeting

    2015-01-14

    Today we had our first official team meeting. We talked about the project and got to know each other.

  • Introductory meeting

    2014-11-25

    This day, last years team held an introductory session about iGEM for interested people.

Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com