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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>  
 
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                 <p><b>Parts for paired dCas9 reporter system</b></p>
 
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                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
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                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
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Revision as of 16:17, 17 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator Liu Wenchao, Xu Luze
 √ BBa_K1689009 Composite C-luc-dCas9 Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
 √ BBa_K1689010 Compostie N-luc-dCas9 Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc Li Xiang, Li Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV Li Xiang, Li Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689007 Composite dCas9-C-luc Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689008 Composite dCas9-N-luc Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, Wei Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, Wei Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, Wei Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, Wei Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, Wei Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, Wei Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP-Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc-dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9-Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc-STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.