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| | <p>Overall, the integration of two general markers allows for the detection of various types of cancers with high reliability and a low false positive rate, while control of signal expression by the bacteria should result in a considerable increase in the signal to noise ratio compared to assay kit-based tests. The direct binding of our MicroBeacons to cancer cells allow for the detection of lactate and AHL in a very controlled microenvironment with minimal crosstalk from other cells in the sample or unbound MicroBeacons. Finally, the use of a fluorescent signal as our output signal will allow for easy measurement from the microfluidic chip through microscopy.</p> | | <p>Overall, the integration of two general markers allows for the detection of various types of cancers with high reliability and a low false positive rate, while control of signal expression by the bacteria should result in a considerable increase in the signal to noise ratio compared to assay kit-based tests. The direct binding of our MicroBeacons to cancer cells allow for the detection of lactate and AHL in a very controlled microenvironment with minimal crosstalk from other cells in the sample or unbound MicroBeacons. Finally, the use of a fluorescent signal as our output signal will allow for easy measurement from the microfluidic chip through microscopy.</p> |
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| − | y="61.803959"
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| − | id="tspan4684">1. Apoptosis is induced only in cancer cells</tspan><tspan
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| − | id="tspan4710"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | y="80.553955"
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| − | x="599.44971">by sTRAIL and causes phosphotadylserine (PS)</tspan><tspan
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | y="99.303955"
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| − | x="599.44971">to flip to the outer membrane.</tspan></text>
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| − | style="font-style:normal;font-weight:normal;font-size:40px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none;stroke-width:1px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
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| − | x="543.49072"
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| − | id="text4714"><tspan
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| − | x="543.49072"
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| − | y="259.81256"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | id="tspan4720">2. Mammalian cells and MicroBeacons are sent</tspan><tspan
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| − | id="tspan4727"
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| − | x="543.49072"
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| − | y="278.56256"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">through the microfluidic chip.</tspan></text>
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| − | style="font-style:normal;font-weight:normal;font-size:40px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none;stroke-width:1px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
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| − | x="728.28436"
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| − | y="376.95023"
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| − | id="text4714-8"><tspan
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| − | id="tspan4752"
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| − | x="728.28436"
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| − | y="376.95023"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">3. MicroBeacons with membrane-</tspan><tspan
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| − | id="tspan4756"
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| − | x="728.28436"
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| − | y="395.70023"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">expressed annexin V bind to exposed</tspan><tspan
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| − | id="tspan4758"
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| − | x="728.28436"
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| − | y="414.45023"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">PS on apoptotic cancer cells and not to</tspan><tspan
| |
| − | id="tspan4760"
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| − | x="728.28436"
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| − | y="433.20023"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">normal cells.</tspan></text>
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| − | <text
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| − | id="text4824"
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| − | y="584.94092"
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| − | x="-0.19042969"
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| − | style="font-style:normal;font-weight:normal;font-size:40px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none;stroke-width:1px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
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| − | xml:space="preserve"><tspan
| |
| − | id="tspan4843"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | y="584.94092"
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| − | x="-0.19042969">4. Higher lactate production rates in the</tspan><tspan
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| − | id="tspan4849"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | y="603.69092"
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| − | x="-0.19042969">apoptotic cancer cell initiates quorum </tspan><tspan
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| − | id="tspan4861"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
| |
| − | y="622.44092"
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| − | x="-0.19042969">sensing between the MicroBeacons to </tspan><tspan
| |
| − | id="tspan9742"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | y="641.19092"
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| − | x="-0.19042969">amplify the signal.</tspan></text>
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| − | <text
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| − | xml:space="preserve"
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| − | style="font-style:normal;font-weight:normal;font-size:40px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none;stroke-width:1px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
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| − | x="723.08173"
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| − | y="594.36316"
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| − | id="text4863"><tspan
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| − | x="723.08173"
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| − | y="594.36316"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial"
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| − | id="tspan4869">5. A green fluorescent signal is</tspan><tspan
| |
| − | id="tspan4878"
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| − | x="723.08173"
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| − | y="613.11316"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">produced by MicroBeacons that have</tspan><tspan
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| − | id="tspan4882"
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| − | x="723.08173"
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| − | y="631.86316"
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| − | style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:15px;font-family:Arial;-inkscape-font-specification:Arial">detected the quorum sensing signals.</tspan></text>
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| | <h3> References </h3> | | <h3> References </h3> |
