Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"
| Line 42: | Line 42: | ||
<td>VR</td> | <td>VR</td> | ||
<td>GTATTACCGCCTTTGAGT</td> | <td>GTATTACCGCCTTTGAGT</td> | ||
| + | </tr> | ||
| + | <tr id="SB-prep-2Ea"> | ||
| + | <td>SB-prep-2Ea</td> | ||
| + | <td>ATGAATTCCAGAAATCATCCTTAGCG</td> | ||
| + | </tr> | ||
| + | <tr id="SB-prep-3P-1"> | ||
| + | <td>SB-prep-3P-1</td> | ||
| + | <td>GCCGCTGCAGTCCGGCAAAAAA</td> | ||
</tr> | </tr> | ||
| Line 47: | Line 55: | ||
<tr class="primers-subheading"> | <tr class="primers-subheading"> | ||
<td colspan="2">Arsenic biosensor</td> | <td colspan="2">Arsenic biosensor</td> | ||
| − | |||
| − | |||
| − | |||
| − | |||
</tr> | </tr> | ||
<tr id="Ars_1_rev"> | <tr id="Ars_1_rev"> | ||
| Line 59: | Line 63: | ||
<td>Ars_2_fwd</td> | <td>Ars_2_fwd</td> | ||
<td>cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC</td> | <td>cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC</td> | ||
| − | |||
| − | |||
| − | |||
| − | |||
</tr> | </tr> | ||
<tr id="Ars_3_fwd"> | <tr id="Ars_3_fwd"> | ||
| Line 71: | Line 71: | ||
<td>Ars_3_rev</td> | <td>Ars_3_rev</td> | ||
<td>tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC</td> | <td>tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC</td> | ||
| + | </tr> | ||
| + | <tr id="ArsO_sfGFP_rev"> | ||
| + | <td>ArsO_sfGFP_rev</td> | ||
| + | <td>ttctttttttttttaaagttaaacaaaattattgttaagtcatatatgtttttgacTCTCCCTATAGTGAGTCGTATTAC</td> | ||
</tr> | </tr> | ||
<tr id="arsRhis_arsR_fwd"> | <tr id="arsRhis_arsR_fwd"> | ||
| Line 87: | Line 91: | ||
<td>arsRhis_Trp_rev</td> | <td>arsRhis_Trp_rev</td> | ||
<td>CTCTAGTATTTCTCCTCTTTCC</td> | <td>CTCTAGTATTTCTCCTCTTTCC</td> | ||
| + | </tr> | ||
| + | <tr id="UTR_noT7_rev"> | ||
| + | <td>UTR_noT7_rev</td> | ||
| + | <td>ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA</td> | ||
</tr> | </tr> | ||
| Line 128: | Line 136: | ||
<td>CATCGACCTCTCCCGCATTAG</td> | <td>CATCGACCTCTCCCGCATTAG</td> | ||
</tr> | </tr> | ||
| − | |||
<tr id="K516030proB_rev"> | <tr id="K516030proB_rev"> | ||
| Line 139: | Line 146: | ||
<td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td> | <td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td> | ||
</tr> | </tr> | ||
| + | |||
| + | |||
<tr class="primers-subheading"> | <tr class="primers-subheading"> | ||
| Line 167: | Line 176: | ||
<td>tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA</td> | <td>tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA</td> | ||
</tr> | </tr> | ||
| + | |||
| + | |||
| + | <tr class="primers-subheading"> | ||
| + | <td colspan="2">Split primers</td> | ||
| + | </tr> | ||
| + | <tr id="Cm_fwd"> | ||
| + | <td>Cm_fwd</td> | ||
| + | <td>CGGCATCAGCACCTTGTC</td> | ||
| + | </tr> | ||
| + | <tr id="Cm_rev"> | ||
| + | <td>Cm_rev</td> | ||
| + | <td>TATACGCAAGGCGACAAG</td> | ||
| + | </tr> | ||
| + | |||
Revision as of 08:39, 17 July 2015
Primers
| Name | Sequence (5´ → 3´) |
|---|---|
| Standard primers | |
| VF | CCACCTGACGTCTAAGAA |
| VR | GTATTACCGCCTTTGAGT |
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA |
| Arsenic biosensor | |
| Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC |
| Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC |
| Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC |
| Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC |
| ArsO_sfGFP_rev | ttctttttttttttaaagttaaacaaaattattgttaagtcatatatgtttttgacTCTCCCTATAGTGAGTCGTATTAC |
| arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC |
| arsRhis_arsR_rev | ctagtattagtggtgatggtgatgatgTTAACTGCAAATGTTCTTACTG |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC |
| UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA |
| Date rape drugs | |
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC |
| pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG |
| pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG |
| K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC |
| K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT |
| gBlocks | |
| GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC |
| GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA |
| GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC |
| GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA |
| Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC |
| Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA |
| Split primers | |
| Cm_fwd | CGGCATCAGCACCTTGTC |
| Cm_rev | TATACGCAAGGCGACAAG |