Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"
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<tr class="primers-subheading"> | <tr class="primers-subheading"> | ||
<td colspan="2">Standard primers</td> | <td colspan="2">Standard primers</td> | ||
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</tr> | </tr> | ||
<tr id="SB-prep-2Ea"> | <tr id="SB-prep-2Ea"> | ||
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<td>SB-prep-3P-1</td> | <td>SB-prep-3P-1</td> | ||
<td>GCCGCTGCAGTCCGGCAAAAAA</td> | <td>GCCGCTGCAGTCCGGCAAAAAA</td> | ||
| + | </tr> | ||
| + | <tr id="VF"> | ||
| + | <td id="VF">VF</td> | ||
| + | <td>CCACCTGACGTCTAAGAA</td> | ||
| + | </tr> | ||
| + | <tr id="VR"> | ||
| + | <td>VR</td> | ||
| + | <td>GTATTACCGCCTTTGAGT</td> | ||
</tr> | </tr> | ||
Revision as of 08:39, 17 July 2015
Primers
| Name | Sequence (5´ → 3´) |
|---|---|
| Standard primers | |
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA |
| VF | CCACCTGACGTCTAAGAA |
| VR | GTATTACCGCCTTTGAGT |
| Arsenic biosensor | |
| Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC |
| Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC |
| Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC |
| Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC |
| ArsO_sfGFP_rev | ttctttttttttttaaagttaaacaaaattattgttaagtcatatatgtttttgacTCTCCCTATAGTGAGTCGTATTAC |
| arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC |
| arsRhis_arsR_rev | ctagtattagtggtgatggtgatgatgTTAACTGCAAATGTTCTTACTG |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC |
| UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA |
| Date rape drugs | |
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC |
| pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG |
| pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG |
| K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC |
| K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT |
| gBlocks | |
| GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC |
| GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA |
| GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC |
| GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA |
| Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC |
| Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA |
| Split primers | |
| Cm_fwd | CGGCATCAGCACCTTGTC |
| Cm_rev | TATACGCAAGGCGACAAG |