Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"
| Line 71: | Line 71: | ||
<td>Ars_3_rev</td> | <td>Ars_3_rev</td> | ||
<td>tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC</td> | <td>tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC</td> | ||
| − | |||
| − | |||
| − | |||
| − | |||
</tr> | </tr> | ||
<tr id="arsRhis_arsR_fwd"> | <tr id="arsRhis_arsR_fwd"> | ||
| Line 91: | Line 87: | ||
<td>arsRhis_Trp_rev</td> | <td>arsRhis_Trp_rev</td> | ||
<td>CTCTAGTATTTCTCCTCTTTCC</td> | <td>CTCTAGTATTTCTCCTCTTTCC</td> | ||
| + | </tr> | ||
| + | <tr id="UTR_arsO_rev"> | ||
| + | <td>UTR_arsO_rev</td> | ||
| + | <td>ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG</td> | ||
</tr> | </tr> | ||
<tr id="UTR_noT7_rev"> | <tr id="UTR_noT7_rev"> | ||
Revision as of 08:58, 17 July 2015
Primers
| Name | Sequence (5´ → 3´) |
|---|---|
| Standard primers | |
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA |
| VF | CCACCTGACGTCTAAGAA |
| VR | GTATTACCGCCTTTGAGT |
| Arsenic biosensor | |
| Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC |
| Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC |
| Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC |
| Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC |
| arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC |
| arsRhis_arsR_rev | ctagtattagtggtgatggtgatgatgTTAACTGCAAATGTTCTTACTG |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC |
| UTR_arsO_rev | ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG |
| UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA |
| Date rape drugs | |
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC |
| pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG |
| pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG |
| K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC |
| K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT |
| gBlocks | |
| GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC |
| GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA |
| GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC |
| GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA |
| Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC |
| Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA |
| Split primers | |
| Cm_fwd | CGGCATCAGCACCTTGTC |
| Cm_rev | TATACGCAAGGCGACAAG |