Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Miscellaneous"
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| − | <p> This Week we started with the <a href="https://2015.igem.org/Tracks/Measurement/Interlab_study" target="_blank">iGEM 2015 Measurement Interlab Study</a>:</p> | + | <p> </U>This Week we started with the <a href="https://2015.igem.org/Tracks/Measurement/Interlab_study" target="_blank">iGEM 2015 Measurement Interlab Study</a></U>:</p> |
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<li>Heat shock transformation of BioBricks from the distribution:</li> | <li>Heat shock transformation of BioBricks from the distribution:</li> | ||
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| − | <p><U>iGEM 2015 Measurement Interlab Study | + | <p><U>iGEM 2015 Measurement Interlab Study</U>:</p> |
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<li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | <li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | ||
| − | <ul><li> | + | <ul><li> 5 ml LB-Medium (37°C)</li></ul> |
<li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)</li> | <li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)</li> | ||
<ul><li> Plasmids stored at -20°C</li></ul> | <ul><li> Plasmids stored at -20°C</li></ul> | ||
Revision as of 17:19, 21 July 2015
Miscellaneous
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- 5 ml LB-Medium (37°C)
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB, 37°C)