Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Miscellaneous"
| Line 192: | Line 192: | ||
<ul> | <ul> | ||
<li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | <li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | ||
| − | <ul><li> 5 ml LB-Medium ( | + | <ul><li> 5 ml LB-Medium + Cm (20 µg/ml), 37°C</li></ul> |
<li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)</li> | <li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)</li> | ||
<ul><li> Plasmids stored at -20°C</li></ul> | <ul><li> Plasmids stored at -20°C</li></ul> | ||
<li>Second heat shock transformation of chem. competent KRX with BBa_I13504</li> | <li>Second heat shock transformation of chem. competent KRX with BBa_I13504</li> | ||
| − | <ul><li> Colonies were used to inoculate over night cultures (5 ml LB, 37°C)</li></ul> | + | <ul><li> Colonies were used to inoculate over night cultures (5 ml LB + Cm20, 37°C)</li></ul> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 210: | Line 210: | ||
<div id="collapseTwelve" class="panel-collapse collapse"> | <div id="collapseTwelve" class="panel-collapse collapse"> | ||
<div class="panel-body"> | <div class="panel-body"> | ||
| − | <p> | + | <p><U>iGEM 2015 Measurement Interlab Study</U>:</p> |
| + | <ul> | ||
| + | <li>Mini-prep of KRX(BBa_I13504)</li> | ||
| + | <ul><li> Plasmid stored at -20°C</li></ul> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 17:32, 21 July 2015
Miscellaneous
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- 5 ml LB-Medium + Cm (20 µg/ml), 37°C
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB + Cm20, 37°C)
iGEM 2015 Measurement Interlab Study:
- Mini-prep of KRX(BBa_I13504)
- Plasmid stored at -20°C