Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminA"

(Created page with "{{Template:Paris_Bettencourt}} <html> <b>Thursday, Jul. 23</b> <ul> <li>Understood the project, just have to do it.</li> </ul> <b>Friday, Jul. 24</b> <ul> <li>Made the project, ...")
 
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{{Template:Paris_Bettencourt}}
 
<html>
 
<html>
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<b>Thursday, Jul. 23</b>
+
<b>Jul. 14th</b>
 +
 
 +
<h3>Goal</h3>
 +
Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).
 +
 
 +
<h3>Procedure</h3>
 
<ul>
 
<ul>
−
<li>Understood the project, just have to do it.</li>
+
<li>Liquid culture overnight in LB.</li>
 +
<li>Centrifuge the tubes for 1 minute with 11000 rpm.</li>
 +
<li>Follow the standard protocol of Thermo Scientific miniprep kit <font color="red">(is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)</font></li>
 +
<li>Measure concentration with Nanodrop</li>
 +
<li>Label the centrifuge tube and store it in the -20 freezer. (g15.35)</li>
 
</ul>
 
</ul>
  
−
<b>Friday, Jul. 24</b>
+
<h3>Results</h3>
 +
Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:
 +
<ul>
 +
<li>tube HO pl. 1 = 431.1 ng/uL</li>
 +
<li>tube HO pl. 2 = 313.6 ng/uL</li>
 +
<li>tube HO pl. 3 = 366.4 ng/uL</li>
 +
<li>tube HO pl. 4 = 261.7 ng/uL</li>
 +
 
 +
<br><br>
 +
<b>Jul. 24th</b>
 
<ul>
 
<ul>
 
<li>Made the project, it is awesome!</li>
 
<li>Made the project, it is awesome!</li>
 
</ul>
 
</ul>

Revision as of 08:59, 23 July 2015

Jul. 14th

Goal

Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).

Procedure

  • Liquid culture overnight in LB.
  • Centrifuge the tubes for 1 minute with 11000 rpm.
  • Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
  • Measure concentration with Nanodrop
  • Label the centrifuge tube and store it in the -20 freezer. (g15.35)

Results

Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:
  • tube HO pl. 1 = 431.1 ng/uL
  • tube HO pl. 2 = 313.6 ng/uL
  • tube HO pl. 3 = 366.4 ng/uL
  • tube HO pl. 4 = 261.7 ng/uL


    • Jul. 24th
    • Made the project, it is awesome!