Difference between revisions of "Team:XJTLU-CHINA"

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Synthetic biology have been used in the aspect of art and created some fantastic works in pervious iGEM competition. This year, the team of iGEM-XJTLU purposed to apply the technology of synthetic biology to draw a color-changed world map to stimulate the process of global warming. The different colors in the map were formed by chromoproteis expressed by genetically modified E coli. Furthermore, we used certain ribothermometers that switch at 37 degree Celsius to control the expression of such chromoproteins. Additionally, Global warming is known as causing glacial ablation, the rise of sea level and the withering of plants. The appearance of the Earth will be altered: the white glaciers melt; the green threes on the land turns into yellow and the coastal areas were flooded with sea water. To simulate this process, a genetic circuit was designed to make the expression of different chromoproteins ordered and variable. Refer to detail information, four different strains sand for different areas on earth were made: “inland”, “coastal”, “marine” and “polar”. For example, the strain that represented for “inland” expressed green chromoprotein when the temperature was below 37 degree Celcius. But when temperature rise to 37 degree Celsius or higher, the “inland” strain expressed yellow chromoprotein which simulated the withering of pants. Also, the rise of sea level was simulated as the color of “coastal” strain changing from green to blue. Therefore, our bio-synthetic world map could directly simulate and display the process of global warming, thus more people can realize the danger of global warming. We hope we could pay attention to claim change and start to protect our own Earth.
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Synthetic biology has been used in the aspect of art and created many fantastic works in previous iGEM competition. This year, the team of iGEM-XJTLU purposed to apply the technology of synthetic biology to draw a bacteria-consisted world map to stimulate the process of global warming.
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Global warming is known as causing glacial ablation, the rise of sea level and the withering of plants. The appearance of the Earth will therefore be altered: the white glaciers melt; the green trees on the land turns into yellow and the coastal areas were flooded with sea water.
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To simulate this process, four strains of genetic modified Escherichia coli (BL21) were designed to make the expression of different chromoproteins ordered and variable.
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37o C RNA thermometer was introduced to our construct to sense the temperature change and control the catalysing of initiation signal of quorum sensing or certain repression proteins.
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For instance, the “inland” strain expressed blue and yellow chromoprotein when the temperature was below 37 degree Celsius and thus exhibit green colour. Nevertheless, when temperature rise to 37 degree Celsius or higher, the pathway of synthetizing of blue chromoprotein was switched off by the direct inhibition of Tet R to pTet. After certain time, yellow chromoprotein will be dominant and thus simulate the withering of pants.
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Therefore, our bio-synthetic world map has the potential to simulate and display the process of global warming, which is an innovative way to promote the effects of global warming. We hope this attempt would draw people attention to climate change and start to protect our own planet Earth.
 
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Project Description

Synthetic biology has been used in the aspect of art and created many fantastic works in previous iGEM competition. This year, the team of iGEM-XJTLU purposed to apply the technology of synthetic biology to draw a bacteria-consisted world map to stimulate the process of global warming. Global warming is known as causing glacial ablation, the rise of sea level and the withering of plants. The appearance of the Earth will therefore be altered: the white glaciers melt; the green trees on the land turns into yellow and the coastal areas were flooded with sea water. To simulate this process, four strains of genetic modified Escherichia coli (BL21) were designed to make the expression of different chromoproteins ordered and variable. 37o C RNA thermometer was introduced to our construct to sense the temperature change and control the catalysing of initiation signal of quorum sensing or certain repression proteins. For instance, the “inland” strain expressed blue and yellow chromoprotein when the temperature was below 37 degree Celsius and thus exhibit green colour. Nevertheless, when temperature rise to 37 degree Celsius or higher, the pathway of synthetizing of blue chromoprotein was switched off by the direct inhibition of Tet R to pTet. After certain time, yellow chromoprotein will be dominant and thus simulate the withering of pants. Therefore, our bio-synthetic world map has the potential to simulate and display the process of global warming, which is an innovative way to promote the effects of global warming. We hope this attempt would draw people attention to climate change and start to protect our own planet Earth.