Difference between revisions of "Team:UCL/Protocols"
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<div id="ligation"><h2>Ligation protocol</h2> | <div id="ligation"><h2>Ligation protocol</h2> | ||
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| − | + | <div id="transformation"><h2>Transformation protocol </h2> | |
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<li>(If using part from the distribution: resuspend the DNA in 10 ul of MiliQ water, making sure that it turns red. Wait 10 minutes before adding the DNA to cells)</li> | <li>(If using part from the distribution: resuspend the DNA in 10 ul of MiliQ water, making sure that it turns red. Wait 10 minutes before adding the DNA to cells)</li> | ||
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<div id="gel"><h2>Agarose gel electrophoresis</h2> | <div id="gel"><h2>Agarose gel electrophoresis</h2> | ||
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<div id="gelextraction"><h2>Assembly of 2 parts using gel extraction</h2> | <div id="gelextraction"><h2>Assembly of 2 parts using gel extraction</h2> | ||
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<div id="gel"><h2>Polymerase Chain Reaction</h2> | <div id="gel"><h2>Polymerase Chain Reaction</h2> | ||
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<li>- Confirm the PCR by running 2 ul of the product on the gel according to the <a href="#gel">gel electrophoresis protocol</a></li> | <li>- Confirm the PCR by running 2 ul of the product on the gel according to the <a href="#gel">gel electrophoresis protocol</a></li> | ||
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Revision as of 20:20, 5 August 2015