Difference between revisions of "Team:Dundee/labjournal manu"

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     <div class="border-week">
 
     <div class="border-week">
 
     <div class="box">
 
     <div class="box">
−
         <div class="labtitle">Week 1</div>
+
         <div class="labtitle">Week Beginning 22/06</div>
 
         <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1"></span>
 
         <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1"></span>
 
           <div class="box-content">
 
           <div class="box-content">
−
           <p class="journal-summary-heading">Week summary</p>
+
           <p class="journal-summary-heading">Summary</p>
 
              
 
              
  
−
         <p class="journal-content">faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.</p>
+
         <p class="journal-content">This week was all about getting started with the newly arrived biobrick  <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a></p>
 
         </div>
 
         </div>
 
         <div id="collapsiblew1" class="collapse week-content">
 
         <div id="collapsiblew1" class="collapse week-content">
 +
 +
        <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div-->
  
  
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       <div class="border-day">
 
       <div class="border-day">
 
         <div class="box">
 
         <div class="box">
−
             <span class="box-title">Day 1</span>
+
             <span class="box-title">Day 1</span> <!--Title of collapsible box-->
 
               <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-1"></span>
 
               <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-1"></span>
 
                 <div id="collapsiblew1-1" class="collapse box-content">
 
                 <div id="collapsiblew1-1" class="collapse box-content">
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−
<!-- POPUP PROTOCOL MODAL SECTION -->
+
<!-- POPUP PROTOCOL MODAL SECTION --
  
−
<!-- Restriction Digest Modal -->
+
<!-- Restriction Digest Modal --
 
<div class="modal fade" id="restriction-digest-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 
<div class="modal fade" id="restriction-digest-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 
   <div class="modal-dialog" role="document">
 
   <div class="modal-dialog" role="document">
Line 300: Line 302:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
-->
  
  
  
 +
<!-- POPUP PROTOCOL MODAL SECTION -->
 +
<!-- PCR Modal -->
 +
<div class="modal fade" id="PCR-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 +
  <div class="modal-dialog" role="document">
 +
    <div class="modal-content">
 +
      <div class="modal-header">
 +
        <button type="button" class="close" data-dismiss="modal" aria-label="close" aria-hidden="true" role="dialog" tabindex="-1"><span aria-hidden="true">&times;</span></button>
 +
        <div class="modal-title" id="myModalLabel">PCR Protocol</div>
 +
      </div>
 +
      <div class="modal-body">
 +
     
 +
<p>
 +
    PCR reaction mixtures were set up according to the table below. The length of all sequences used is stated in the <strong>Sequences</strong> section.
 +
</p>
 +
<table border="1" cellspacing="0" cellpadding="0">
 +
 +
    <tbody color="black">
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    <strong>Reactants</strong>
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    <strong>Volume (µl)</strong>
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    DNA
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    DMSO
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    2.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    Forward Primer
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    0.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    Reverse Primer
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    0.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    Herculase Buffer
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    10
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    dNTP
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    0.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    Herculase
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    0.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    H<sub>2</sub>O
 +
                </p>
 +
            </td>
 +
            <td width="312" valign="top">
 +
                <p align="center">
 +
                    34.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<p>
 +
    **PCR Programme**
 +
</p>
 +
<p>
 +
    Elongation time was altered according to the length of the sequences.
 +
</p>
 +
 +
      </div>
 +
      <div class="modal-footer">
 +
        <button type="button" class="btn btn-primary" data-dismiss="modal">Close</button>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
 +
 +
 +
<!-- Restriction Digest Modal -->
 +
<div class="modal fade" id="restriction-digest-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 +
  <div class="modal-dialog" role="document">
 +
    <div class="modal-content">
 +
      <div class="modal-header">
 +
        <button type="button" class="close" data-dismiss="modal" aria-label="close" aria-hidden="true" role="dialog" tabindex="-1"><span aria-hidden="true">&times;</span></button>
 +
        <div class="modal-title" id="myModalLabel">Restriction Digest Protocol</div>
 +
      </div>
 +
      <div class="modal-body">
 +
        <p>
 +
Restriction digests were set up according to the table below. The appropriate reaction and restriction enzymes that were used is stated in the    <strong>Journal</strong> section.
 +
</p>
 +
<table border="1" cellspacing="0" cellpadding="0">
 +
    <tbody>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    <strong>Post Plasmid Purification</strong>
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    <strong>Post PCR</strong>
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    <strong>gBlock</strong>
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong>DNA</strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    1mg
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    50µl
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    100ng
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong>Cutsmart Buffer (µl)</strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    6
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    6
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    6
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong>Water (µl)</strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    As required
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    As required
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong>Enzyme 1 (µl)</strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong>Enzyme 2 (µl)</strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    1.5
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="164" valign="top">
 +
                <p>
 +
                    <strong><u>Total Volume (µl)</u></strong>
 +
                </p>
 +
            </td>
 +
            <td width="170" valign="top">
 +
                <p>
 +
                    30
 +
                </p>
 +
            </td>
 +
            <td width="151" valign="top">
 +
                <p>
 +
                    60
 +
                </p>
 +
            </td>
 +
            <td width="139" valign="top">
 +
                <p>
 +
                    30
 +
                </p>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<p>
 +
    <img
 +
        width="628"
 +
        height="34"
 +
        src="file:///C:/Users/Lucy/AppData/Local/Temp/msohtmlclip1/01/clip_image001.png"
 +
        alt="Text Box: Table x: The components of the reaction digest reactions and their respective quantities."
 +
    />
 +
    <strong><u></u></strong>
 +
</p>
 +
<p>
 +
    <br/>
 +
    The digests were incubated at 37<sup>o</sup>C for 3 hours.
 +
    <br/>
 +
    Note that when performing plasmid digestions, 2.5µl of alkaline phosphatase and 2.5µl of its buffer was added at the 2 hour and 2.5 hour mark.
 +
</p>
 +
 +
</div>
 +
      </div>
 +
      <div class="modal-footer">
 +
        <button type="button" class="btn btn-primary" data-dismiss="modal">Close</button>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
<!-- Ligation Modal -->
 +
<div class="modal fade" id="ligation-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 +
  <div class="modal-dialog" role="document">
 +
    <div class="modal-content">
 +
      <div class="modal-header">
 +
        <button type="button" class="close" data-dismiss="modal" aria-label="close" aria-hidden="true" role="dialog" tabindex="-1"><span aria-hidden="true">&times;</span></button>
 +
        <div class="modal-title" id="myModalLabel">Ligation Protocol</div>
 +
      </div>
 +
      <div class="modal-body">
 +
<p>
 +
Ligation reactions were set up according to the table below. The vector to insert ratio is stated for each specific reaction in the    <strong>Journal</strong> section.
 +
</p>
 +
<table border="1" cellspacing="0" cellpadding="0">
 +
    <tbody>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    <strong>Vector (µl)</strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    <strong>2:1 (Vector: Insert) [µl]</strong>
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    <strong>3:1 (Vector: Insert) [µl]</strong>
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    <strong>4:1 (Vector: Insert) [µl]</strong>
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
                <p>
 +
                    <strong>T4 DNA Ligase </strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
                <p>
 +
                    <strong>Ligase Buffer </strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
                <p>
 +
                    <strong>Vector </strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    1
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
                <p>
 +
                    <strong>Insert</strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    -
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    2
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    3
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    4
 +
                </p>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td width="140" valign="top">
 +
                <p>
 +
                    <strong>H<sub>2</sub>0</strong>
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    7
 +
                </p>
 +
            </td>
 +
            <td width="111" valign="top">
 +
                <p align="center">
 +
                    5
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    4
 +
                </p>
 +
            </td>
 +
            <td width="129" valign="top">
 +
                <p align="center">
 +
                    3
 +
                </p>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<p>
 +
    <img
 +
        width="628"
 +
        height="34"
 +
        src="file:///C:/Users/Lucy/AppData/Local/Temp/msohtmlclip1/01/clip_image001.png"
 +
        alt="Text Box: Table x: The components of the ligation reactions and their respective quantities."
 +
    />
 +
</p>
 +
<p>
 +
    <br/>
 +
    The reactions were incubated at room temperature for at least 3 hours.
 +
</p>
 +
</div>
 +
      <div class="modal-footer">
 +
        <button type="button" class="btn btn-primary" data-dismiss="modal">Close</button>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
 +
<!-- Transformation Modal -->
 +
<div class="modal fade" id="transformation-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
 +
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 +
        <div class="modal-title" id="myModalLabel">Transformation Protocol</div>
 +
      </div>
 +
      <div class="modal-body"><p>
 +
    1. 100µl of competent cells were defrosted. Thaw on ice.
 +
</p>
 +
<p>
 +
    2. Agar plates were taken out of 4<sup>o</sup>C to warm to room temperature.
 +
</p>
 +
<p>
 +
    3. 1-5µl of DNA was added to cells.
 +
</p>
 +
<p>
 +
    4. Cell and DNA mixture was left on ice for 20 minutes.
 +
</p>
 +
<p>
 +
    5. Mixture was placed in 42<sup>o</sup>C water bath for 90 seconds.
 +
</p>
 +
<p>
 +
    6. Mixture was put back on ice for 2 minutes.
 +
</p>
 +
<p>
 +
    7. 1ml of LB (without antibiotic) was added to cells.
 +
</p>
 +
<p>
 +
    8. Cells were allowed to grow for 1 hour at 37<sup>o</sup>C in a shaking incubator.
 +
</p>
 +
<p>
 +
    9. Cells were then spun down and the supernatant discarded.
 +
</p>
 +
<p>
 +
    10. The pellet was resuspended in the 100µl.
 +
</p>
 +
<p>
 +
    Cells were plated on agar plates with appropriate antibiotic.
 +
</p>
 +
</div>
 +
      </div>
 +
      <div class="modal-footer">
 +
        <button type="button" class="btn btn-primary" data-dismiss="modal">Close</button>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
 +
<!-- SDS-PAGE + Western Blot Modal -->
 +
<div class="modal fade" id="sds-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
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 +
        <div class="modal-title" id="myModalLabel">Protein Expression Optimization Protocol</div>
 +
      </div>
 +
      <div class="modal-body">
 +
<p>
 +
    <strong><u>SDS-PAGE and Western Blotting </u></strong>
 +
</p>
 +
<p>
 +
    1. 50µl of an overnight culture was used to inoculate 5ml of fresh LB containing the appropriate antibiotics and left to grow at 37<sup>o</sup>C in a
 +
    shaking incubator.
 +
</p>
 +
<p>
 +
    2. Once the OD<sub>600</sub>=0.6-0.8, 1mM of IPTG was added to the culture and left to grow for a further 3 hours.
 +
</p>
 +
<p>
 +
    3. 1ml of each culture was taken and spun down in a microcentrifuge for 3 minutes.
 +
</p>
 +
<p>
 +
    4. Meanwhile, 950µl of 2x Laemmli sample buffer was added to 50µl of B-mercaptoethanol.
 +
</p>
 +
<p>
 +
    5. The supernatant was discarded from the samples and the pellet resuspended in 200µl of the above solution.
 +
</p>
 +
<p>
 +
    6. The samples were then boiled at 90<sup>o</sup>C for 10 minutes.
 +
</p>
 +
<p>
 +
    7. Afterwards, they were spun down for 1 minute in a microcentrifuge.
 +
</p>
 +
<p>
 +
    8. At this stage, the samples could be stored at -20<sup>o</sup>C or ran on a gel immediately.
 +
</p>
 +
<p>
 +
    9. Two gradient gels consisting of a 12% separating gel and a stacking gel were prepared and the samples ran at 100V until proteins reach end of the
 +
    stacking gel at which point the voltage was increased to 200V for 20 minutes.
 +
</p>
 +
<p>
 +
    10. One gel was stained using Instant Blue and the other was used to transfer the proteins onto a membrane.
 +
</p>
 +
<p>
 +
    11. After the proteins had been transferred onto the membrane, the membrane was blocked in 5% Marvel milk overnight at 4<sup>o</sup>C.
 +
</p>
 +
<p>
 +
    12. The next day, the membrane was washed 3 times in 1xTBST buffer and then suspended in 10ml of 1xTBS buffer to which the primary anti-His antibody was
 +
    added and left for one hour. The protein ladder was cut off before adding the primary antibody.
 +
</p>
 +
<p>
 +
    13. The membrane was then washed 3 times in 1xTBST buffer for 5 minutes and then suspended in 10ml of 1xTBS buffer, to which the secondary antibody was
 +
    added and left for one hour.
 +
</p>
 +
<p>
 +
    14. The membrane was then washed in 3x10ml 1xTBST buffer for 5 minutes.
 +
</p>
 +
<p>
 +
    15. The blot was then soaked in developing solution for 5 minutes before being wrapped in a plastic cover aligned with the protein ladder that was cut off
 +
    at step 12.
 +
</p>
 +
<p>
 +
    Once in the developing room, the film was exposed to the blot for 30 seconds, 2 minutes and 5 minutes and then placed in the film developer.
 +
</p>
 +
 +
      </div>
 +
      <div class="modal-footer">
 +
        <button type="button" class="btn btn-primary" data-dismiss="modal">Close</button>
 +
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 +
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 +
</div>
 +
 +
 +
<!-- lucy's image modals
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 +
        <div class="modal-title" id="myModalLabel">Figure 1: LbpA PCR. The image shows the gel ran of the LbpA PCR product. The expected size of the fragment was 2832bp which corresponds to the observed band on the gel.</div>
 +
      </div>
 +
      <div class="modal-body">
 +
      <img src="https://static.igem.org/mediawiki/2015/8/85/Dundee15-LbpAPCR-pSB1C3.jpg" style="width:400px;height:auto">'
 +
 +
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<div class="modal fade" id="lbpa-figure2-modal" tabindex="-1" role="dialog" aria-labelledby="myModalLabel">
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 +
        <div class="modal-title" id="myModalLabel">Figure 2: LbpA PCR. The image shows the gel ran of the LbpA PCR product. The expected size of the fragment was 2832bp which corresponds to the observed band on the gel.</div>
 +
      </div>
 +
      <div class="modal-body">
 +
      <img src="https://static.igem.org/mediawiki/2015/d/d4/LbpA_PCR_pqe80_.jpg" style="width:400px;height:auto">'
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<!-- JQUERY -->
 
<!-- JQUERY -->

Revision as of 17:30, 6 August 2015

LABJOURNAL

BioSpray

Our forensic toolkit aims to use synthetic biology approaches to improve on the current methods used by crime scene investigators.

Chromium Detector

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Fingerprint Aging

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Week Beginning 22/06

Summary

This week was all about getting started with the newly arrived biobrick BBa_K1058008

Day 1

Aim of experiment: ultricies nec tellus eu

Protocols Used: Click here to see our restriction digest protocol


faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 2

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 3

Aim of experiment:Storage of BBa_K1058008

Protocols Used:

The agar stab received from the registry was transferred to an agar plate supplemented with 2x Chloramphenicol. The plate was incubated at 37°C for 16h and then stored at 4°C. Furthermore, an overnight culture was set up by inoculating 5ml LB, supplemented with chloramphenicol, with the agar stab. The overnight culture was incubated for 16h.

Day 4

Aim of experiment:Purification and sequencing of BBa_K1058008

Protocols Used:

The overnight culture was retrieved and centrifuged at 4000rpm for 10min. The plasmid, pSB1C3, containing BBa_K1058008 was purified according to the miniprep protocol. The concentration was determined by nanodrop and found to be 190.9ng/µl. 2 Samples were submitted for sequencing, each containing 550ng DNA, 2µl of 3mM primer, and water filled up to a total volume of 30µl

Day 5

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Week 2

Week summary

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 1

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 2

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 3

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 4

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 5

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.