Difference between revisions of "Team:UNITN-Trento/Safety"

(Prototype team page)
 
(v 0.1)
Line 1: Line 1:
−
{{UNITN-Trento}}
 
 
<html>
 
<html>
−
<h2>Safety in iGEM</h2>
+
</p></div>
 +
<meta name="viewport" content="width=device-width, initial-scale=1" />
 +
 +
</html>{{UNITN-Trento/globalIncluder}}<html>
 +
<script src="https://2015.igem.org/Template:UNITN-Trento/JSModernizer?action=raw&ctype=text/javascript"></script>
 +
<script src="https://2015.igem.org/Template:UNITN-Trento/JSscrollTo?action=raw&ctype=text/javascript"></script>
 +
 +
 +
 +
<div id="super_wrap">
 +
<div class="no-sidebar">
 +
<div id="page-wrapper">
  
−
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
<!-- Header -->
 +
<header id="header"></html>
 +
{{UNITN-Trento/mainMenu}}
 +
<html></header>
 +
<!-- Main -->
 +
 +
<section id="cta" data-stellar-background-ratio="0.4" style="background-image:url('https://static.igem.org/mediawiki/2015/8/89/Unitn_pics_cta_notebook.jpg');">
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
<header>
 +
<h2><strong>Work hard, Work safe</strong></h2>
 +
<p> ? </p>
 +
</header>
 +
</section>
 +
 +
 +
 +
<article id="main">
 +
<section class="wrapper style1 container special" style="margin-top:3em;">
 +
<div class="row">
 +
<div class="4u 12u(narrower)">
  
 +
<section>
 +
<header>
 +
<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>
 +
</header>
 +
<p>We have reviewed our organisms and parts against the <a class="i_linker" href="https://2015.igem.org/Safety/WhiteList" target="_blank">White List</a>. All the parts and bacterial strains that we used are in the <span class="i_enph">Risk Group 1</span></p>
 +
</section>
  
−
<h4>Safe Project Design</h4>
+
</div>
 +
<div class="4u 12u(narrower)">
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<section>
 +
<header>
 +
<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>
 +
</header>
 +
<p>We have submitted to iGEM headquarters <span class="i_enph">“About Our Lab”</span> and <span class="i_enph">“About Our Project”</span> questionnaires by the June 26<sup>th</sup> deadline</p>
 +
</section>
  
−
<ul>
+
</div>
−
<li>Choosing a non-pathogenic chassis</li>
+
<div class="4u 12u(narrower)">
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
  
−
<h4>Safe Lab Work</h4>
+
<section>
 +
<header>
 +
<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>
 +
</header>
 +
<p>We have submitted to iGEM headquarters the <span class="i_enph">“Final Safety Form”</span> by the August 28<sup>th</sup> deadline</p>
 +
</section>
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
</div>
 +
</div>
 +
</section>
 +
 +
 +
<!-- One -->
 +
<section class="wrapper style4 container" style="margin-top:1em;">
  
−
<h4>Safe Shipment</h4>
+
<!-- Content -->
 +
<div class="content">
 +
<section>
 +
<header>
 +
<h3>About Safety</h3>
 +
</header>
 +
 
 +
<p>We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:</p>
 +
<ul class="checklist">
 +
<li>General considerations on risks and prevention</li>
 +
<li>Electrical Hazard</li>
 +
<li>Chemical Hazard</li>
 +
<li>Biological Hazard</li>
 +
<li>Compressed gasses and cryogenic liquids</li>
 +
</ul>
 +
<p>We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.</p>
 +
</section>
 +
</div>
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
</section>
 +
 +
<section class="wrapper style4 container" style="margin-top:1em;">
  
 +
<!-- Content -->
 +
<div class="content">
 +
<section>
 +
<!--<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>-->
 +
<header>
 +
<h3>Safe Project Design</h3>
 +
</header>
 +
<!-- TODO -->
 +
 +
<div class="row">
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">Who will use your product? What opinions do these people have about your project?</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">If your product is successful, who will receive benefits and who will be harmed?</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">What happens when it's all used up? Will it be sterilized, discarded, or recycled?</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">Is it safer, cheaper, or better than other technologies that do the same thing?</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
<div class="6u 12u(narrower)">
 +
<section>
 +
<header>
 +
<p class="question">__</p>
 +
</header>
 +
<p>Answer</p>
 +
</section>
 +
</div>
 +
</div> 
 +
</section>
 +
</div>
 +
</section>
 +
 +
 +
<section class="wrapper style4 container" style="margin-top:1em;">
  
−
</div>
+
<!-- Content -->
 +
<div class="content">
 +
<section>
 +
<header>
 +
<h3>Working Safely... with Parts</h3>
 +
</header>
 +
<!-- TODO -->
 +
 +
<p>All the parts we used throughout the project are NOT toxic or dangerous for  humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.</p>
 +
<p>We did <span class="i_enph">NOT</span> use any virulence factors in our organisms.</p>
 +
<p>The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.</p>
 +
 +
<header>
 +
<h3>Working Safely... with Organisms</h3>
 +
</header>
 +
<p>All the bacterial strains used are from Risk Group 1. We used the following strains:</p>
 +
<ul class="checklist">
 +
<li> One Shot® TOP10 Chemically Competent E. coli</li>
 +
<li> K12 - NEB 10-beta E. coli</li>
 +
<li> K12 - JM109 E. coli</li>
 +
<li> BL21 - NEB Express E. coli</li>
 +
</ul>
 +
</section>
 +
</div>
 +
</section>
 +
 +
 +
<section class="wrapper style4 container" style="margin-top:1em;">
 +
 
 +
<!-- Content -->
 +
<div class="content">
 +
<section>
 +
<header>
 +
<h3>Personal Protective Equipment (PPE)</h3>
 +
</header>
 +
 +
<p>Last but non least we adopted the appropriate PPE during the lab work.  We always used lab coats, nitrile gloves and goggles.  We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1.8.</p>
 +
<p>We used chemicals that are associated with hazard (ethidium bromide, azide,  organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.</p>
 +
<p>We carefully revised all the Material Safety Datasheet of each compound that was  used prior to start an experiment.</p> 
 +
</section>
 +
</div>
 +
</section>
 +
 
 +
<!-- Two -->
 +
 +
 
 +
</article>
 +
 
 +
 
 +
<!-- Footer -->
 +
</html>{{UNITN-Trento/mainFooter}}<html>
 +
 
 +
</div>
 +
 
 +
<!-- Scripts -->
 +
</div>
 +
</div>
 
</html>
 
</html>

Revision as of 13:11, 10 August 2015

Work hard, Work safe

?

We have reviewed our organisms and parts against the White List. All the parts and bacterial strains that we used are in the Risk Group 1

We have submitted to iGEM headquarters “About Our Lab” and “About Our Project” questionnaires by the June 26th deadline

We have submitted to iGEM headquarters the “Final Safety Form” by the August 28th deadline

About Safety

We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:

  • General considerations on risks and prevention
  • Electrical Hazard
  • Chemical Hazard
  • Biological Hazard
  • Compressed gasses and cryogenic liquids

We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.

Safe Project Design

Who will use your product? What opinions do these people have about your project?

Answer

Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?

Answer

If your product is successful, who will receive benefits and who will be harmed?

Answer

What happens when it's all used up? Will it be sterilized, discarded, or recycled?

Answer

Is it safer, cheaper, or better than other technologies that do the same thing?

Answer

__

Answer

Working Safely... with Parts

All the parts we used throughout the project are NOT toxic or dangerous for humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.

We did NOT use any virulence factors in our organisms.

The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.

Working Safely... with Organisms

All the bacterial strains used are from Risk Group 1. We used the following strains:

  • One Shot® TOP10 Chemically Competent E. coli
  • K12 - NEB 10-beta E. coli
  • K12 - JM109 E. coli
  • BL21 - NEB Express E. coli

Personal Protective Equipment (PPE)

Last but non least we adopted the appropriate PPE during the lab work. We always used lab coats, nitrile gloves and goggles. We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1.8.

We used chemicals that are associated with hazard (ethidium bromide, azide, organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.

We carefully revised all the Material Safety Datasheet of each compound that was used prior to start an experiment.