Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

m
Line 95: Line 95:
 
                 <td>UTR_noT7_rev</td>
 
                 <td>UTR_noT7_rev</td>
 
                 <td>ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA</td>
 
                 <td>ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA</td>
 +
            </tr>
 +
 +
<tr class="primers-subheading">
 +
                <td colspan="2">CFPS</td>
 +
            </tr>
 +
          <tr id="Mut_gap_UTR_fwd">
 +
                <td>Mut_gap_UTR_fwd</td>
 +
                <td>AGAATAATCTATGCGTAAAGGCGAAGAG</td>
 +
            </tr>
 +
<tr id="pBS1C3_RraA_pre">
 +
                <td>pBS1C3_RraA_pre</td>
 +
                <td>ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC</td>
 +
            </tr>
 +
<tr id="pBS1C3_RraA_suf">
 +
                <td>pBS1C3_RraA_suf</td>
 +
                <td>TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG</td>
 +
            </tr>
 +
<tr id="pBS1C3_RraB_pre">
 +
                <td>pBS1C3_RraB_pre</td>
 +
                <td>TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC</td>
 +
            </tr>
 +
<tr id="pBS1C3_RraB_suf">
 +
                <td>pBS1C3_RraB_suf</td>
 +
                <td>GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG</td>
 +
            </tr>
 +
<tr id="pZ9_pT7_rne_fwd">
 +
                <td>pZ9_pT7_rne_fwd</td>
 +
                <td>gtccgcaacctgttgagtaaCTGCAGGAAGGGGATCCG</td>
 +
            </tr>
 +
<tr id="pZ9_pT7_rne_rev ">
 +
                <td>pZ9_pT7_rne_rev</td>
 +
                <td>tagtatttctcctctttctctagtatctcCCTATAGTGAGTCGTATTAC</td>
 +
            </tr>
 +
 +
 +
<tr id="rne_qPCR_fwd1">
 +
                <td>rne_qPCR_fwd1</td>
 +
                <td>GTGCTCGGCATAAGCCGAAG</td>
 +
            </tr>
 +
<tr id="rne_qPCR_fwd2">
 +
                <td>rne_qPCR_fwd2</td>
 +
                <td>GCATAAGCCGAAGATATCG</td>
 +
            </tr>
 +
<tr id="rne_qPCR_fwd3">
 +
                <td>rne_qPCR_fwd3</td>
 +
                <td>CCGAAGATATCGGTAGAGTT</td>
 +
            </tr>
 +
<tr id="rne_qPCR_rev1">
 +
                <td>rne_qPCR_fwd4</td>
 +
                <td>CTGCGCTACGGTAGCGAAAC</td>
 +
            </tr>
 +
<tr id="rne_qPCR_rev2">
 +
                <td>rne_qPCR_rev2</td>
 +
                <td>TTTGCGCCTCGTTATCATCC</td>
 +
            </tr>
 +
<tr id="rne_to_pZ9_fwd">
 +
                <td>rne_to_pZ9_fwd</td>
 +
                <td>GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC </td>
 +
            </tr>
 +
<tr id="rne_to_pZ9_rev">
 +
                <td>rne_to_pZ9_rev</td>
 +
                <td>CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG</td>
 +
            </tr>
 +
<tr id="Rra_purif_fwd">
 +
                <td>Rra_purif_fwd</td>
 +
                <td>TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA </td>
 +
            </tr>
 +
<tr id="RraA_fwd">
 +
                <td>RraA_fwd</td>
 +
                <td>ATGAAATACGATACTTCCGAGCTTTG</td>
 +
            </tr>
 +
<tr id="RraA_purif_rev">
 +
                <td>RraA_purif_rev</td>
 +
                <td>ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG</td>
 +
            </tr>
 +
<tr id="RraA_rev">
 +
                <td>RraA_rev</td>
 +
                <td>TCATTCAATATCCAGCGGATCTTC</td>
 +
            </tr>
 +
<tr id="RraB_fwd">
 +
                <td>RraB_fwd</td>
 +
                <td>ATGGCAAACCCGGAACAACTG</td>
 +
            </tr>
 +
<tr id="RraB_purif_fwd">
 +
                <td>RraB_purif_fwd</td>
 +
                <td>GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA</td>
 +
            </tr>
 +
 +
<tr id="RraB_purif_rev">
 +
                <td>RraB_purif_rev</td>
 +
                <td>TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG </td>
 +
            </tr>
 +
<tr id="RraB_rev">
 +
                <td>RraB_rev</td>
 +
                <td>TTAGTGGCGAACTCCGTCATC</td>
 +
            </tr>
 +
<tr id="SYFP_only_fwd">
 +
                <td>SYFP_only_fwd</td>
 +
                <td>ATGGTTAGCAAGGGCGAAGA</td>
 +
            </tr>
 +
<tr id="SYFP_only_rev">
 +
                <td>SYFP_only_rev</td>
 +
                <td>TTATTATTTATACAGCTCATCCATACC</td>
 +
            </tr>
 +
<tr id="UTR_fwd">
 +
                <td>UTR_fwd</td>
 +
                <td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC </td>
 +
            </tr>
 +
<tr id="UTR_rev">
 +
            <td>UTR_rev</td>    <td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG </td>
 +
                <td></td>
 +
            </tr>
 +
<tr id="UTR_RFP_fwd">
 +
                <td>UTR_RFP_fwd</td>
 +
                <td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC</td>
 +
            </tr>
 +
<tr id="UTR_SYFP_fwd">
 +
                <td>UTR_SYFP_fwd</td>
 +
                <td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC</td>
 
             </tr>
 
             </tr>
  
  
 
<tr class="primers-subheading">
 
<tr class="primers-subheading">
 +
 
                 <td colspan="2">Date rape drugs</td>
 
                 <td colspan="2">Date rape drugs</td>
 
             </tr>
 
             </tr>

Revision as of 22:30, 13 August 2015

iGEM Bielefeld 2015


Primers

Name Sequence (5´ → 3´)
Standard primers
SB-prep-2Ea ATGAATTCCAGAAATCATCCTTAGCG
SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA
VF CCACCTGACGTCTAAGAA
VR GTATTACCGCCTTTGAGT
Arsenic biosensor
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_3_fwd cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC
Ars_3_rev tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC
arsRhis_arsR_fwd tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC
arsRhis_arsR2_r ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG
arsRhis_Trp_fwd CATCATCACCATCACCAC
arsRhis_Trp_rev CTCTAGTATTTCTCCTCTTTCC
UTR_arsO_rev ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG
UTR_noT7_rev ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA
CFPS
Mut_gap_UTR_fwd AGAATAATCTATGCGTAAAGGCGAAGAG
pBS1C3_RraA_pre ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraA_suf TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG
pBS1C3_RraB_pre TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraB_suf GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG
pZ9_pT7_rne_fwd gtccgcaacctgttgagtaaCTGCAGGAAGGGGATCCG
pZ9_pT7_rne_rev tagtatttctcctctttctctagtatctcCCTATAGTGAGTCGTATTAC
rne_qPCR_fwd1 GTGCTCGGCATAAGCCGAAG
rne_qPCR_fwd2 GCATAAGCCGAAGATATCG
rne_qPCR_fwd3 CCGAAGATATCGGTAGAGTT
rne_qPCR_fwd4 CTGCGCTACGGTAGCGAAAC
rne_qPCR_rev2 TTTGCGCCTCGTTATCATCC
rne_to_pZ9_fwd GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC
rne_to_pZ9_rev CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG
Rra_purif_fwd TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA
RraA_fwd ATGAAATACGATACTTCCGAGCTTTG
RraA_purif_rev ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG
RraA_rev TCATTCAATATCCAGCGGATCTTC
RraB_fwd ATGGCAAACCCGGAACAACTG
RraB_purif_fwd GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA
RraB_purif_rev TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG
RraB_rev TTAGTGGCGAACTCCGTCATC
SYFP_only_fwd ATGGTTAGCAAGGGCGAAGA
SYFP_only_rev TTATTATTTATACAGCTCATCCATACC
UTR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC
UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG
UTR_RFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC
UTR_SYFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG
psB_BlcRfwd_new" CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
BlcC_rev_new TTAGTCTTTCTGCGATCGGTACCC
bclR-rv" GTCTTTCTGCGATCGGTACCC
blcR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT
BlcB_mut_fwd CCTTTCGAGTTCCGCACCGTTCCCT
BlcB_mut_rev" AGGGAACGGTGCGGAACTCGAAAGG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
K60_BlcPshort_r GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC
K60_BlcPshort_f" GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC
bclR-rv GTCTTTCTGCGATCGGTACCC
pSB1C3blcOnewfw AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA
Split primers
Cm_fwd CGGCATCAGCACCTTGTC
Cm_rev TATACGCAAGGCGACAAG

The gene-specific part of the primer is written in uppercase letters, while lowercase letters denote an overhang.