|
|
| Line 14: |
Line 14: |
| | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); | | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); |
| | -webkit-transform-style: preserve-3d; | | -webkit-transform-style: preserve-3d; |
| − | background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
| + | background-color: transparent; |
| − | background-color: rgb(198, 169, 117); | + | |
| | } | | } |
| | </style> | | </style> |
| Line 21: |
Line 20: |
| | | | |
| | <body class="gwd-body-kwce"> | | <body class="gwd-body-kwce"> |
| − | <title>wiki</title>
| |
| − | <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
| |
| − | <meta name="generator" content="Google Web Designer 1.3.2.0521">
| |
| − | <style type="text/css">
| |
| − | html, body {
| |
| − | width: 100%;
| |
| − | height: 100%;
| |
| − | margin: 0px;
| |
| − | }
| |
| − | body {
| |
| − | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
| |
| − | -webkit-transform-style: preserve-3d;
| |
| − | background-color: transparent;
| |
| − | }
| |
| − | </style>
| |
| | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> | | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
| | <title></title> | | <title></title> |
| Line 237: |
Line 221: |
| | .gwd-a-1thu { | | .gwd-a-1thu { |
| | color: rgb(0, 0, 0); | | color: rgb(0, 0, 0); |
| − | -webkit-transform: translate3d(0px, 4.34px, 0px);
| |
| − | -webkit-transform-style: preserve-3d;
| |
| | background-image: none; | | background-image: none; |
| | background-color: rgb(255, 255, 255); | | background-color: rgb(255, 255, 255); |
| Line 569: |
Line 551: |
| | <div class="gwd-div-2ed9"></div> | | <div class="gwd-div-2ed9"></div> |
| | <div class="gwd-div-7xzo gwd-a-1thu"> | | <div class="gwd-div-7xzo gwd-a-1thu"> |
| − | <h1>Project </h1> | + | <h1>Parts </h1> |
| − | <big><span class="gwd-span-3t71"><span class="gwd-span-fn2e">Our project is about </span><span class="gwd-span-a1dh"><span class="gwd-span-6ea3" lang="EN-US"><span style="">celiac</span></span> | + | <big>Throughout the project we started to learn the fundamental |
| − | <span class="gwd-span-xrze"><span class="gwd-span-bh3g" lang="EN-US"> </span><span class="gwd-span-1fs0"><span class="gwd-span-5vwr" lang="EN-US"><span style="">disease, this is a
| + | principles of synthetic biology to get to work on our plasmid with |
| − | enteropathy, autoimmune affecting individuals who possess genes HLA-DQ8
| + | which we want to see gluten degradation via the enzyme Kumamax. For |
| − | or
| + | this we going to insert in an e. coli the parts (Biobricks) needed to |
| − | HLA-DQ2, where T cells attack the body when the gliadins and glutenins
| + | make our future bacteria can degrade gluten. The parts are:<br> |
| − | are
| + | |
| − | absorbed by the small intestine, in others words, celiac patients have
| + | |
| − | a
| + | |
| − | permanent intolerance to gluten-containing foods.</span></span></span><span class="gwd-span-1haj" lang="EN-US"></span></span></span></span></big><big><span style="" lang="EN-US"> <br>
| + | |
| − | <br>
| + | |
| − | A strict
| + | |
| − | diet throughout life gluten-free allows to the small intestine get
| + | |
| − | health.
| + | |
| − | Obvious sources of gluten are bread, many cereals for breakfast, pasta,
| + | |
| − | pizza,
| + | |
| − | cakes and biscuits. The gluten can also be used to give structure to
| + | |
| − | the food
| + | |
| − | products, such as sausages, stock cubes, soups and sauces. Naturally
| + | |
| − | the
| + | |
| − | gluten-free foods include meat, fish, eggs, fruits, vegetables, dairy | + | |
| − | products,
| + | |
| − | beans, potatoes, rice, corn, quinoa and buckwheat. Food substitutes as
| + | |
| − | the | + | |
| − | especially gluten-free<span style=""> </span>bread,
| + | |
| − | flour,
| + | |
| − | pasta and biscuits, have increased in recent years in variety and are
| + | |
| − | available
| + | |
| − | by prescription in some countries.<br>
| + | |
| − | <br>
| + | |
| − | </span></big>
| + | |
| − | <p style="text-align: left;" class="MsoNormal"><big><span style="" lang="EN-US"><o:p></o:p></span></big><span style="" lang="EN-US"><big>But what
| + | |
| − | makes this a problem?, well the problem is the cost of this diet, which
| + | |
| − | often
| + | |
| − | are priced high in gluten-free foods, besides the clear deprivation the
| + | |
| − | patient
| + | |
| − | celiac without meals as common as pizza, and even drinks such as beer.
| + | |
| − | So we
| + | |
| − | think of a way to solve the gap between celiac patients and those
| + | |
| − | persons
| + | |
| − | without eating disorders, but without forgetting the price, therefore,
| + | |
| − | we also
| + | |
| − | consider the low cost of production and, like that, was born the idea
| + | |
| − | of
| + | |
| − | creating a pill that is able to degrade gluten before its contact with
| + | |
| − | the
| + | |
| − | enterocytes.</big></span>
| + | |
| − | <br>
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <div style="text-align: center;">
| + | |
| − | <img alt="File:Cientific explain.jpg" src="https://static.igem.org/mediawiki/2015/thumb/f/f6/Cientific_explain.jpg/716px-Cientific_explain.jpg" height="600" width="716">
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <p style="text-align: left;" class="MsoNormal"><big><span style="" lang="EN-US">When the
| + | |
| − | gluten enter in contact with the villi of the small intestine
| + | |
| − | (enterocytes) the | + | |
| − | helper T cells (type of reporter) detects the gluten as a toxic
| + | |
| − | substance and
| + | |
| − | leaves a chemical trail, which is followed by antibodies, among the
| + | |
| − | more
| + | |
| − | important<span style=""> </span>the
| + | |
| − | Transglutaminase and the
| + | |
| − | Antigliadin (in this case); In addition to the lymphocytes T and B. The
| + | |
| − | first (
| + | |
| − | lymphocytes T) would be the natural killers and an army of these cells
| + | |
| − | will
| + | |
| − | attack to the infected cells, which could cause a cancerous effect, in
| + | |
| − | this
| + | |
| − | case the dangerous cells are the enterocytes, and after of the atack
| + | |
| − | they could
| + | |
| − | causing inflammation, poor absorption, and other symptoms. But does not
| + | |
| − | stop
| + | |
| − | there the auto-immune action, also the B cells serves as a kind of
| + | |
| − | memory which
| + | |
| − | is used to the next time that the threat enter in the body, the reply
| + | |
| − | will be
| + | |
| − | more quickly and efficiently.<o:p></o:p></span></big>
| + | |
| − | </p>
| + | |
| − | <div style="text-align: left;"><big>
| + | |
| | </big> | | </big> |
| − | <br>
| + | <ul> |
| − | </div> | + | <li><big><span style="font-family: 'Arial','sans-serif';"><strong style="font-weight: bold;">Promoter |
| − | </div> | + | <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a href="http://parts.igem.org/Part:BBa_J23119"><span style="font-weight: bold;">)</span></a>: |
| − | <span class="gwd-span-3t71"><span class="gwd-span-a1dh"><span class="gwd-span-xrze"><span class="gwd-span-1haj" lang="EN-US"> | + | </span>Constitutive |
| − | </span></span> | + | promoter (which works permanently) that is give in the relative |
| − | </span> | + | fluorescence of these plasmids in the TG1 strain grown in LB medium.</big> |
| − | </span> | + | </li> |
| | + | <li><big><strong style="font-weight: bold;">RBS</strong><strong style="font-weight: bold;"> |
| | + | <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>: |
| | + | Synthetic RBS with uplifting sequence</big>.</li> |
| | + | <li><big><strong>Vector:<span style="font-weight: normal;"> <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big> |
| | + | </li> |
| | + | <li><big><strong><span style="font-family: 'Arial','sans-serif';">Coding Region: |
| | + | KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong> |
| | + | It |
| | + | degrades gluten, |
| | + | celiac disease leading cause. Enzyme generated by rational mutation for |
| | + | the active site of it. It was created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington |
| | + | 2011.</a></big> |
| | + | </li> |
| | + | <li><big><strong><span style="font-family: 'Arial','sans-serif';">Reporter: RFP |
| | + | <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span style="font-family: Arial,sans-serif;"> Red f</span>luorescence |
| | + | protein.</big> |
| | + | </li> |
| | + | <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator |
| | + | <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong> |
| | + | </span>Dual terminator consisting of |
| | + | the B0010 and B0012 parties. It serves to give greater efficiency in |
| | + | transcription</big>.</li> |
| | + | </ul> |
| | <div style="text-align: center;"> | | <div style="text-align: center;"> |
| − | <img src="https://static.igem.org/mediawiki/2015/4/44/Symptoms_of_celiac_disease.jpg" class="gwd-img-c7yq"> | + | <img alt="File:Parts.jpg" src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" height="59" width="288"> |
| | <br> | | <br> |
| | + | <img alt="File:Circuito.jpg" src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg" height="309" width="800"> |
| | <br> | | <br> |
| − | <div style="text-align: left;"><span class="gwd-span-3t71"><span class="gwd-span-u82g"><big>The | + | <div style="text-align: left;"><big>This is a |
| − | damage caused by the autoimmune reaction can cause
| + | graphic model of as it has be our plasmid where we can visualize the |
| − | histological damage in the villi, and a biopsy of these is the
| + | promoter, the RBS, the enzyme KumaMax, the RFP and the terminator, |
| − | means of diagnosis of this pathology. The only current treatment is a | + | joined by means of prefixes and suffixes that indicate the locations of |
| − | diet without gluten, which is expensive and difficult to follow.</big><br>
| + | court.</big> |
| − | <br>
| + | <br> |
| − | </span></span>
| + | |
| − | <div style="text-align: center;"><span class="gwd-span-3t71"><span class="gwd-span-u82g"> </span></span> | + | |
| − | <img src="https://static.igem.org/mediawiki/2015/0/0a/Histological_study.jpg" class="gwd-img-5j7g">
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| | <br> | | <br> |
| − | <span class="gwd-span-3t71">
| |
| − | <div class="gwd-div-rxi8"><big><span class="gwd-span-oxe1">This is the tip of the iceberg of
| |
| − | celiac disease, the symptoms as are easy to diagnose, but there are
| |
| − | other four ways where the symptoms are not so clear. These forms are:<br class="gwd-br-q8fj">
| |
| − | </span>
| |
| − | </big>
| |
| − | <ol class="gwd-ol-ewzq">
| |
| − | <li class="gwd-li-cx4k"><big><span class="gwd-span-bp36">Silent Celiac Disease: Patients have
| |
| − | no symptoms but if damage histological.</span> </big>
| |
| − | </li>
| |
| − | <li class="gwd-li-83gw"><big><span class="gwd-span-y410">Latent Celiac Disease: There are not
| |
| − | symptoms and stunted villi. Individuals tend to develop the disease
| |
| − | suddenly.</span> </big>
| |
| − | </li>
| |
| − | <li class="gwd-li-hrrk"><big><span class="gwd-span-9f33">Potential Celiac Disease: Are subjects
| |
| − | with high genetic risk that could have the disease.</span> </big>
| |
| − | </li>
| |
| − | <li class="gwd-li-7d5y"><big><span class="gwd-span-juom">Refractory Celiac Disease: Those
| |
| − | patients diagnosted celiac and despite follow a diet without gluten
| |
| − | doesn't improve and can get diseases even more serious.</span></big>
| |
| − | </li>
| |
| − | </ol>
| |
| | <div style="text-align: center;"> | | <div style="text-align: center;"> |
| − | <img src="https://static.igem.org/mediawiki/2015/3/37/Iceberg_CD.jpg" class="gwd-img-74jg"> | + | <img alt="File:Plasmido.jpg" src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg" height="600" width="664"> |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <div style="text-align: left;">
| + | |
| − | <div style="text-align: left;"><big><span style="" lang="EN-US">The celiac disease are associated with many
| + | |
| − | diseases, these can also be complications of refractory patients. But is<span style=""> </span>important know the variety
| + | |
| − | of diseases that
| + | |
| − | can produces by de celiac disease: <o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
| + | |
| − | <br>
| + | |
| − | </span>- Food
| + | |
| − | intolerance problems.<br>
| + | |
| − | <o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""></span>-
| + | |
| − | Variety of cancers.<o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
| + | |
| − | </span>-
| + | |
| − | Diseases related to the nervous system.<o:p></o:p></span></big>
| + | |
| − | <big><span style="" lang="EN-US"><span style=""><br>
| + | |
| − | </span>- Diseases related to the circulatory system.<o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
| + | |
| − | </span></span>-
| + | |
| − | Skin-related illnesses.</big>
| + | |
| − | <o:p></o:p>
| + | |
| − | <big><span class="gwd-span-3t71"><span class="gwd-span-h4j2"></span></span></big>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| − | <div style="text-align: center;">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/c/c3/Complications.jpg" class="gwd-img-4j8q">
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <div style="text-align: left;">
| + | |
| − | <h1>Statistics</h1>
| + | |
| − | <div style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; text-align: center; background-color: rgb(255, 255, 255);">
| + | |
| − | <br class="Apple-interchange-newline">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/7/75/Celiaco.jpg" alt="celiaco.jpg" title="celiaco.jpg" style="border: medium none ; vertical-align: middle; float: left; width: 503px; height: 466px;">
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| − | <div style="text-align: left;"><big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255);"> In
| + | |
| − | our country Chile, the statistics are the following:</big><span style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; display: inline ! important; float: none; background-color: rgb(255, 255, 255);"></span>
| + | |
| − | </div>
| + | |
| − | <ul style="margin: 0.3em 0px 0px 1.5em; padding: 0px; line-height: 19.05px; list-style-type: square; list-style-image: url(https://2015.igem.org/wiki/skins/igem/bullet.gif); color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255); text-align: left;">
| + | |
| − | <li style="margin-bottom: 0.1em;"><big>The
| + | |
| − | probability of having celiac disease is 1 each 265.</big>
| + | |
| − | </li>
| + | |
| − | <li style="margin-bottom: 0.1em;"><big>The celiac
| + | |
| − | patients represent 15% of the Chilean population.</big>
| + | |
| − | </li>
| + | |
| − | <li style="margin-bottom: 0.1em;"><big>Most die by
| + | |
| − | cancer without really knowing their status.</big>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| | </div> | | </div> |
| − | </span>
| |
| | </div> | | </div> |
| − | </div>
| + | <big><br> |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <span style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 15.24px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; display: inline ! important; float: none; background-color: rgb(255, 255, 255);"><br>
| + | |
| − | <br>
| + | |
| − | But Europe have the contries with more celiacs internationally, also
| + | |
| − | they are the places with more deaths by this pathology and the regions
| + | |
| − | than more consuming wheat in the world.</span><span style="" lang="EN-US"> <big>In
| + | |
| − | Europe it is estimated that the 1% of adults and children have the
| + | |
| − | disease. The
| + | |
| − | prevalence varies widely; for ages 30 to 64 years, that is eight times
| + | |
| − | higher
| + | |
| − | in Finland (2.4%) than in Germany (0.3%), perhaps relating both to
| + | |
| − | genetic and
| + | |
| − | environmental factors. In Finland, the prevalence has doubled in 20
| + | |
| − | years.<o:p></o:p></big></span><big><br>
| + | |
| | </big> | | </big> |
| − | <br>
| |
| − | <div style="text-align: center;">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/thumb/d/de/Statistics.jpg/800px-Statistics.jpg" alt="File:Statistics.jpg">
| |
| − | <br>
| |
| − | <br>
| |
| − | <div style="text-align: left;"><big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255);">Since
| |
| − | the main gene pool of people with this mutation originated in Northern
| |
| − | Europe, all of their descendents throughout North America, Australia,
| |
| − | South Africa, etc. are at a higher risk for inheriting this genetic
| |
| − | predisposition to the disease. </big><span id="result_box" class="" style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255);"
| |
| − | lang="en"><big><span class="hps">For
| |
| − | example</span><span class="Apple-converted-space"> </span><span class="hps">in the United States</span><span>,</span><span class="Apple-converted-space"> </span><span class="hps">the prevalence</span><span class="Apple-converted-space"> </span>
| |
| − | <span class="hps">of celiac disease</span><span class="Apple-converted-space"> </span><span class="hps">by</span><span class="Apple-converted-space"> </span><span class="hps">ethnic</span><span class="Apple-converted-space"> </span>
| |
| − | <span class="hps">race</span>
| |
| − | <span class="Apple-converted-space"> </span>
| |
| − | </big><span class="hps"><big>is:</big></span></span>
| |
| − | <br>
| |
| − | </div>
| |
| − | <br>
| |
| − | <img src="https://static.igem.org/mediawiki/2015/thumb/7/7f/Prevalence.jpg/800px-Prevalence.jpg" alt="File:Prevalence.jpg">
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| | </div> | | </div> |
| − | <strong></strong>
| |
| − | </div>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <div class="gwd-div-a9c8"></div>
| |
| − | <br>
| |
| | <br> | | <br> |
| | <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww"> | | <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww"> |
| Line 829: |
Line 617: |
| | <br> | | <br> |
| | <br> | | <br> |
| − | <div class="gwd-div-t5nw gwd-div-bcgj"> | + | <div class="gwd-div-t5nw gwd-div-fola"> |
| | <style> | | <style> |
| | body { | | body { |
| Line 890: |
Line 678: |
| | -webkit-transform-style: preserve-3d; | | -webkit-transform-style: preserve-3d; |
| | } | | } |
| − | .gwd-div-bcgj { | + | .gwd-div-fola { |
| − | -webkit-transform: translate3d(-8.03px, -5682.85px, 0px); | + | -webkit-transform: translate3d(-7.67px, -1851.29px, 0px); |
| | -webkit-transform-style: preserve-3d; | | -webkit-transform-style: preserve-3d; |
| | } | | } |
