Team:EPF Lausanne/Notebook/Yeast
saccharomyces cerevisiae
pTPGI_dCas9_VP64 integration
As we need dCas9 expressing yeasts, we decided to integrate a plasmid that would inducively express dCas9.
First and second trial
The negative and positive control worked fine, but the transformation control didn’t work. After investigation we found out that we didn’t have an origin of replication in our plasmid thus not enabling our yeasts to replicate the plasmid and to grow. See more details here