Team:ETH Zurich/Modeling/Parameters

"What I cannot create I do not understand."
- Richard Feynmann

Parameters

AHL module

Single cell model

Name Description Value References/Estimation
\(K_{d,\text{LuxRAHL}}\)100 nMDissociation constant between luxR and AHLWeber, 2013
\(\text{LuxR}_\text{tot}\)0.0025 μMTotal concentration of LuxR estimated
\(a_\mathrm{LuxI}\)1 μM.min-1Maximal production rate of LuxIBasu, 2005
\(k_\mathrm{leaky}\)0.0005 μM-1Leakiness of PLuxR promoter estimated
\(K_\mathrm{a,LuxRAHL}\)0.01 μMActivation coefficient of LuxRAHL estimated
\(d_\mathrm{LuxI}\)0.0167 min-1Degradation rate of LuxI estimated
\(a_\mathrm{AHL}\)0.04 μM.min-1Production rate of AHL Weber, 2013
\(d_\mathrm{AHL}\)0.01 min-1Degradation rate of AHLBasu, 2005
\(v_\mathrm{AiiA}\)\(k_\mathrm{cat} \cdot C_\mathrm{AiiA} \)Maximal conversion rate of AiiA
\(k_\mathrm{cat}\)1.63 103>/SUP>min-1Maximal conversion rate of AiiA
\(C_\mathrm{AiiA}\)0.05 μMConcentration of AiiA

Lactate module

Single cell model

Assumptions

Name Description Value References/Estimation
\(\text{Lac}_{\text{ini}}\) Initial concentration of lactate in the medium 1 μM Low concentration of lactate in the medium
Kdllini Initial concentration of lactate in the medium