"What I cannot create I do not understand."
- Richard Feynmann

Parameters

AHL module

Name	Description	Value	References/Estimation
\(K_{d,\text{LuxRAHL}}\)	100 nM	Dissociation constant between luxR and AHL	Weber, 2013
\(\text{LuxR}_\text{tot}\)	0.0025 μM	Total concentration of LuxR	estimated
\(a_\mathrm{LuxI}\)	1 μM.min^-1	Maximal production rate of LuxI	Basu, 2005
\(k_\mathrm{leaky}\)	0.0005 μM^-1	Leakiness of P_LuxR promoter	estimated
\(K_\mathrm{a,LuxRAHL}\)	0.01 μM	Activation coefficient of LuxRAHL	estimated
\(d_\mathrm{LuxI}\)	0.0167 min^-1	Degradation rate of LuxI	estimated
\(a_\mathrm{AHL}\)	0.04 μM.min^-1	Production rate of AHL	Weber, 2013
\(d_\mathrm{AHL}\)	0.01 min^-1	Degradation rate of AHL	Basu, 2005
\(v_\mathrm{AiiA}\)	\(k_\mathrm{cat} \cdot C_\mathrm{AiiA} \)	Maximal conversion rate of AiiA
\(k_\mathrm{cat}\)	1.63 10³min^-1	Turnover number of AiiA	Wang, 2004
\(C_\mathrm{AiiA}\)	0.05 μM	Concentration of AiiA
\(K_\mathrm{M,AiiA}\)	2.95 10³ μM	of AiiA	Wang, 2004
\(a_\mathrm{GFP}\)	2 μM.min^-1	Maximal production rate of GFP	Basu, 2005
\(d_\mathrm{GFP}\)	0.01 min^-1	Degradation rate of GFP	estimated

Name	Description	Minimum Value	Maximum Value	References/Estimation
\(\text{B}}\)	Initial concentration of lactate in the medium	0.1 μM	2 μM	Low concentration of lactate in the medium
\(\text{Lac}_{\text{ini}}\)	Initial concentration of lactate in the medium	0.1 μM	2 μM	Low concentration of lactate in the medium
\(K_\mathrm{d,DLL}\)	Dissociation constant between the dimer of Lldr and Lactate	10 (μM)²	10000 (μM)²